Works matching IS 17542189 AND DT 2021 AND VI 16 AND IP 2

Results: 29

Detection of aberrant gene expression events in RNA sequencing data.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1276, doi. 10.1038/s41596-020-00462-5

By:

Yépez, Vicente A.;
Mertes, Christian;
Müller, Michaela F.;
Klaproth-Andrade, Daniela;
Wachutka, Leonhard;
Frésard, Laure;
Gusic, Mirjana;
Scheller, Ines F.;
Goldberg, Patricia F.;
Prokisch, Holger;
Gagneur, Julien

Publication type:

Article

Precision genome editing using cytosine and adenine base editors in mammalian cells.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1089, doi. 10.1038/s41596-020-00450-9

By:

Huang, Tony P.;
Newby, Gregory A.;
Liu, David R.

Publication type:

Article

Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1251, doi. 10.1038/s41596-020-00457-2

By:

Zhang, Jitao;
Scarcelli, Giuliano

Publication type:

Article

Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1193, doi. 10.1038/s41596-020-00454-5

By:

Kit Leng Lui, Sarah;
Keegan, Sarah;
Tonzi, Peter;
Kahli, Malik;
Chen, Yu-Hung;
Chalhoub, Noor;
Coleman, Kate E.;
Fenyo, David;
Smith, Duncan J.;
Huang, Tony T.

Publication type:

Article

Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1170, doi. 10.1038/s41596-020-00453-6

By:

Kim, Daesik;
Kang, Beum-Chang;
Kim, Jin-Soo

Publication type:

Article

Characterization of connective tissues using near-infrared spectroscopy and imaging.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1297, doi. 10.1038/s41596-020-00468-z

By:

Afara, Isaac O.;
Shaikh, Rubina;
Nippolainen, Ervin;
Querido, William;
Torniainen, Jari;
Sarin, Jaakko K.;
Kandel, Shital;
Pleshko, Nancy;
Töyräs, Juha

Publication type:

Article

Reaction screening in multiwell plates: high-throughput optimization of a Buchwald–Hartwig amination.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1152, doi. 10.1038/s41596-020-00452-7

By:

Cook, Adam;
Clément, Roxanne;
Newman, Stephen G.

Publication type:

Article

Decoding personal biotic and abiotic airborne exposome.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1129, doi. 10.1038/s41596-020-00451-8

By:

Jiang, Chao;
Zhang, Xinyue;
Gao, Peng;
Chen, Qiong;
Snyder, Michael

Publication type:

Article

τ-SGA: synthetic genetic array analysis for systematically screening and quantifying trigenic interactions in yeast.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1219, doi. 10.1038/s41596-020-00456-3

By:

Kuzmin, Elena;
Rahman, Mahfuzur;
VanderSluis, Benjamin;
Costanzo, Michael;
Myers, Chad L.;
Andrews, Brenda J.;
Boone, Charles

Publication type:

Article

Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryos.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1062, doi. 10.1038/s41596-020-00449-2

By:

Rosàs-Canyelles, Elisabet;
Modzelewski, Andrew J.;
Geldert, Alisha;
He, Lin;
Herr, Amy E.

Publication type:

Article

High-resolution, ultrasensitive and quantitative DNA double-strand break labeling in eukaryotic cells using i-BLESS.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1034, doi. 10.1038/s41596-020-00448-3

By:

Biernacka, Anna;
Skrzypczak, Magdalena;
Zhu, Yingjie;
Pasero, Philippe;
Rowicka, Maga;
Ginalski, Krzysztof

Publication type:

Article

Stem-cell-derived human microglia transplanted into mouse brain to study human disease.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 1013, doi. 10.1038/s41596-020-00447-4

By:

Fattorelli, Nicola;
Martinez-Muriana, Anna;
Wolfs, Leen;
Geric, Ivana;
De Strooper, Bart;
Mancuso, Renzo

Publication type:

Article

Recombinant extracellular vesicles as biological reference material for method development, data normalization and assessment of (pre-)analytical variables.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 603, doi. 10.1038/s41596-020-00446-5

By:

Geeurickx, Edward;
Lippens, Lien;
Rappu, Pekka;
De Geest, Bruno G.;
De Wever, Olivier;
Hendrix, An

Publication type:

Article

On-resin multicomponent protocols for biopolymer assembly and derivatization.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 561, doi. 10.1038/s41596-020-00445-6

By:

Rivera, Daniel G.;
Ricardo, Manuel G.;
Vasco, Aldrin V.;
Wessjohann, Ludger A.;
Van der Eycken, Erik V.

Publication type:

Article

High-throughput cultivation and identification of bacteria from the plant root microbiota.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 988, doi. 10.1038/s41596-020-00444-7

By:

Zhang, Jingying;
Liu, Yong-Xin;
Guo, Xiaoxuan;
Qin, Yuan;
Garrido-Oter, Ruben;
Schulze-Lefert, Paul;
Bai, Yang

Publication type:

Article

Facile synthesis of per(6-O-tert-butyldimethylsilyl)-α-, β-, and γ-cyclodextrin as protected intermediates for the functionalization of the secondary face of the macrocycles.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 965, doi. 10.1038/s41596-020-00443-8

By:

Benkovics, Gábor;
Malanga, Milo;
Cutrone, Giovanna;
Béni, Szabolcs;
Vargas-Berenguel, Antonio;
Casas-Solvas, Juan Manuel

Publication type:

Article

Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 937, doi. 10.1038/s41596-020-00442-9

By:

Xie, Ruoxiao;
Liang, Zhe;
Ai, Yongjian;
Zheng, Wenchen;
Xiong, Jialiang;
Xu, Peidi;
Liu, Yupeng;
Ding, Mingyu;
Gao, Jianyi;
Wang, Jiaping;
Liang, Qionglin

Publication type:

Article

Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 919, doi. 10.1038/s41596-020-00441-w

By:

Koike, Hiroyuki;
Iwasawa, Kentaro;
Ouchi, Rie;
Maezawa, Mari;
Kimura, Masaki;
Kodaka, Asuka;
Nishii, Shozo;
Thompson, Wendy L.;
Takebe, Takanori

Publication type:

Article

Generation of neighbor-labeling cells to study intercellular interactions in vivo.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 872, doi. 10.1038/s41596-020-00438-5

By:

Ombrato, Luigi;
Nolan, Emma;
Passaro, Diana;
Kurelac, Ivana;
Bridgeman, Victoria L.;
Waclawiczek, Alexander;
Duarte, Delfim;
Lo Celso, Cristina;
Bonnet, Dominique;
Malanchi, Ilaria

Publication type:

Article

Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 841, doi. 10.1038/s41596-020-00437-6

By:

Ellis, Peter;
Moore, Luiza;
Sanders, Mathijs A.;
Butler, Timothy M.;
Brunner, Simon F.;
Lee-Six, Henry;
Osborne, Robert;
Farr, Ben;
Coorens, Tim H. H.;
Lawson, Andrew R. J.;
Cagan, Alex;
Stratton, Mike R.;
Martincorena, Inigo;
Campbell, Peter J.

Publication type:

Article

Analysis framework and experimental design for evaluating synergy-driving gene expression.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 812, doi. 10.1038/s41596-020-00436-7

By:

Schrode, Nadine;
Seah, Carina;
Deans, P. J. Michael;
Hoffman, Gabriel;
Brennand, Kristen J.

Publication type:

Article

Labeling and tracking of immune cells in ex vivo human skin.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 791, doi. 10.1038/s41596-020-00435-8

By:

Dijkgraaf, Feline E.;
Toebes, Mireille;
Hoogenboezem, Mark;
Mertz, Marjolijn;
Vredevoogd, David W.;
Matos, Tiago R.;
Teunissen, Marcel B. M.;
Luiten, Rosalie M.;
Schumacher, Ton N.

Publication type:

Article

Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 893, doi. 10.1038/s41596-020-00439-4

By:

Narasimhan, Siddarth;
Pinto, Cecilia;
Lucini Paioni, Alessandra;
van der Zwan, Johan;
Folkers, Gert E.;
Baldus, Marc

Publication type:

Article

A surgical mouse model of neonatal pressure overload by transverse aortic constriction.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 775, doi. 10.1038/s41596-020-00434-9

By:

Malek Mohammadi, Mona;
Abouissa, Aya;
Heineke, Joerg

Publication type:

Article

A robust unsupervised machine-learning method to quantify the morphological heterogeneity of cells and nuclei.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 754, doi. 10.1038/s41596-020-00432-x

By:

Phillip, Jude M.;
Han, Kyu-Sang;
Chen, Wei-Chiang;
Wirtz, Denis;
Wu, Pei-Hsun

Publication type:

Article

OCTAD: an open workspace for virtually screening therapeutics targeting precise cancer patient groups using gene expression features.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 728, doi. 10.1038/s41596-020-00430-z

By:

Zeng, Billy;
Glicksberg, Benjamin S.;
Newbury, Patrick;
Chekalin, Evgeny;
Xing, Jing;
Liu, Ke;
Wen, Anita;
Chow, Caven;
Chen, Bin

Publication type:

Article

Implementation of a 4Pi-SMS super-resolution microscope.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 677, doi. 10.1038/s41596-020-00428-7

By:

Wang, Jingyu;
Allgeyer, Edward S.;
Sirinakis, George;
Zhang, Yongdeng;
Hu, Kevin;
Lessard, Mark D.;
Li, Yiming;
Diekmann, Robin;
Phillips, Michael A.;
Dobbie, Ian M.;
Ries, Jonas;
Booth, Martin J.;
Bewersdorf, Joerg

Publication type:

Article

Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 634, doi. 10.1038/s41596-020-00427-8

By:

Lee, Kang Soo;
Pereira, Fátima C.;
Palatinszky, Márton;
Behrendt, Lars;
Alcolombri, Uria;
Berry, David;
Wagner, Michael;
Stocker, Roman

Publication type:

Article

Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development.

Published in:

Nature Protocols, 2021, v. 16, n. 2, p. 579, doi. 10.1038/s41596-020-00433-w

By:

Giandomenico, Stefano L.;
Sutcliffe, Magdalena;
Lancaster, Madeline A.

Publication type:

Article