Works matching Gene expression

Results: 5000
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    Identification of Hub Genes Associated With Development and Microenvironment of Hepatocellular Carcinoma by Weighted Gene Co-expression Network Analysis and Differential Gene Expression Analysis.

    Published in:
    Frontiers in Genetics, 2020, v. 11, p. N.PAG, doi. 10.3389/fgene.2020.615308
    By:
    • Bai, Qingquan;
    • Liu, Haoling;
    • Guo, Hongyu;
    • Lin, Han;
    • Song, Xuan;
    • Jin, Ye;
    • Liu, Yao;
    • Guo, Hongrui;
    • Liang, Shuhang;
    • Song, Ruipeng;
    • Wang, Jiabei;
    • Qu, Zhibo;
    • Guo, Huaxin;
    • Jiang, Hongchi;
    • Liu, Lianxin;
    • Yang, Haiyan
    Publication type:
    Article
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    Investigating the Role of Hub Calcification Proteins in Atherosclerosis via Integrated Transcriptomics and Network-Based Approach Atherosclerosis (AS) is a chronic inflammatory condition of the arteries, characterized by plaque formation that can restrict blood flow and lead to potentially fatal cardiovascular events. Given that AS is responsible for a quarter of global deaths, this study aimed to develop a systematic bioinformatics approach to identify biomarkers and regulatory targets involved in plaque development, with the goal of reducing cardiovascular disease risk. AS-specific mRNA expression profiles were retrieved from a publicly accessible database, followed by differentially expressed genes (DEGs) identification and AS-specific weighted gene co-expression network (WGCN) construction. Thereafter, calcification and atherosclerosis-specific (CASS) DEGs were utilized for protein–protein interaction network (PPIN) formation, followed by gene ontology (GO) term and pathway enrichment analyses. Lastly, AS-specific 3-node miRNA feed-forward loop (FFL) construction and analysis was performed. Microarray datasets GSE43292 and GSE28829 were obtained from gene expression omnibus (GEO). A total of 3785 and 6176 DEGs were obtained in case of GSE28829 and GSE43292; 3256 and 5962 module DEGs corresponding to GSE28829 and GSE43292 were obtained from WGCN. From a total of 54 vascular calcification (VC) genes, 20 and 29 CASS-DEGs corresponding to GSE28829 and GSE43292 were overlapped. As observed from FFL centrality measures, the highest-order subnetwork motif comprised one TF (SOX7), one miRNA (miR-484), and one mRNA (SPARC) in the case of GSE28829. Also, in the case of GSE43292, the highest-order subnetwork motif comprised one TF (ESR2), one miRNA (miR-214-3p), and one mRNA (MEF2C). These findings have important implications for developing new therapeutic strategies for AS. The identified TFs and miRNAs may serve as potential therapeutic targets for treating atheros

    Published in:
    Biology (2079-7737), 2024, v. 13, n. 11, p. 867, doi. 10.3390/biology13110867
    By:
    • Alharbi, Hajed Obaid A.;
    • Khan, Asifa;
    • Rahmani, Arshad Husain
    Publication type:
    Article
    34

    Gene Expression Analysis for Drought Tolerance in Early Stage of Potato Plant Development Drought has increasingly affected the yield of Solanum tuberosum L. (potato) every year over the last decade, posing serious economic problems for the global agricultural industry. Therefore, it is important to research drought tolerance in plants and obtain more robust varieties of crops. The aim of the present work was to study the expression of drought-upregulated genes in drought-tolerant and drought-sensitive varieties of potato. Bioreactors were used to identify whether each variety was drought-tolerant or drought-sensitive; then, expression analysis was performed according to the morphological characteristics of the plantlets in two different media: Murashige and Skoog (MS) medium and MS medium with 20% PEG-6000 to simulate osmotic stress. Based on the quantitative parameters of six initial varieties, two varieties were selected (Gala and Aksor) for further gene expression analysis. The expression of genes commonly upregulated in drought (ER24, TAS14, DREB147315, PP2C, 102605413 and NF-YC4) was higher in the drought-tolerant variety than in the sensitive one. Therefore, the expression of these genes can be used to determine the drought tolerance of a potato variety in vitro in the early plant development stage. Moreover, comparative analysis showed that some of the targeted genes used to identify drought tolerance in this study are conserved across different plant species.

    Published in:
    Biology (2079-7737), 2024, v. 13, n. 11, p. 857, doi. 10.3390/biology13110857
    By:
    • Kanat, Rakhim;
    • Shamekova, Malika;
    • Sapakhova, Zagipa;
    • Toishimanov, Maxat;
    • Daurov, Dias;
    • Raissova, Nurgul;
    • Abilda, Zhanar;
    • Daurova, Ainash;
    • Zhambakin, Kabyl
    Publication type:
    Article
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