Works matching DE "SITE-specific mutagenesis"

Results: 1374

Comparative analysis of DNA binding and abiotic stress tolerance of Dps All4145 and its homologs in Anabaena PCC 7120.

Published in:

Molecular Biology Reports, 2025, v. 52, n. 1, p. 1, doi. 10.1007/s11033-025-10590-4

By:

Singh, Shilpi;
Rai, Krishna Kumar;
Shankar, Alka;
Dubey, Santosh Kumar;
Raj, Alka;
Rai, Ruchi

Publication type:

Article

Modification of carbonyl reductase based on substrate pocket loop regions alteration: an application for synthesis of duloxetine chiral intermediate in high efficiency.

Published in:

Bioprocess & Biosystems Engineering, 2025, v. 48, n. 6, p. 927, doi. 10.1007/s00449-025-03152-2

By:

Zhang, Xiao-Jian;
Chen, Kai-Li;
Li, Xiang-Yang;
Yuan, Qi-Qi;
Lin, Chao-Ping;
Liu, Zhi-Qiang;
Zheng, Yu-Guo

Publication type:

Article

CONSTRUCT: an algorithmic tool for identifying functional or structurally important regions in protein tertiary structure Open Access.

Published in:

Bioinformatics, 2025, v. 41, n. 4, p. 1, doi. 10.1093/bioinformatics/btaf166

By:

Chivot, Lucas;
Mathieux, Noé;
Cosson, Anna;
Bridier-Nahmias, Antoine;
Favennec, Loïc;
Gelly, Jean-Christophe;
Clain, Jérôme;
Coppée, Romain

Publication type:

Article

Alteration of the Catalytic Properties of the Epoxyalcohol Synthase CYP443D1 (NvEAS) of the Starlet Sea Anemone Nematostella vectensis as a Result of a Single Amino Acid Substitution.

Published in:

Doklady Biochemistry & Biophysics, 2025, v. 520, n. 1, p. 42, doi. 10.1134/S160767292460057X

By:

Gorina, S. S.;
Lantsova, N. V.;
Toporkova, Y. Y.;
Grechkin, A. N.

Publication type:

Article

Echinacoside Ameliorates UVB-Induced Skin Damage Through Selective Inhibition of the Cutaneous TRPV3 Channel.

Published in:

Molecules, 2025, v. 30, n. 9, p. 2026, doi. 10.3390/molecules30092026

By:

Mo, Shilun;
Yue, Xinying;
Qu, Yaxuan;
Zhang, Guoji;
Wang, Liqin;
Sun, Xiaoying

Publication type:

Article

Methanogenesis marker 16 metalloprotein is the primary coenzyme M synthase in Methanosarcina acetivorans.

Published in:

PLoS Genetics, 2025, v. 21, n. 5, p. 1, doi. 10.1371/journal.pgen.1011695

By:

Chadwick, Grayson L.;
Williams, Madison C.;
Shalvarjian, Katie E.;
Nayak, Dipti D.

Publication type:

Article

Functional Identification of Catalytic Metal Ion Binding Sites within RNA.

Published in:

PLoS Biology, 2005, v. 3, n. 8, p. 1, doi. 10.1371/journal.pbio.0030277

By:

Hougland, James L;
Kravchuk, Alexander V;
Herschlag, Daniel;
Piccirilli, Joseph A

Publication type:

Article

Functional Characterisation of Highly Conserved and Structurally Prominent Residues of 2‐Methylisoborneol Synthase.

Published in:

Chemistry - A European Journal, 2023, v. 29, n. 32, p. 1, doi. 10.1002/chem.202300775

By:

Gu, Binbin;
Dickschat, Jeroen S.

Publication type:

Article

Infrared Spectroscopy Elucidates the Inhibitor Binding Sites in a Metal‐Dependent Formate Dehydrogenase.

Published in:

Chemistry - A European Journal, 2022, v. 28, n. 54, p. 1, doi. 10.1002/chem.202201091

By:

Laun, Konstantin;
Duffus, Benjamin R.;
Wahlefeld, Stefan;
Katz, Sagie;
Belger, Dennis;
Hildebrandt, Peter;
Mroginski, Maria Andrea;
Leimkühler, Silke;
Zebger, Ingo

Publication type:

Article

Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus.

Published in:

Photosynthesis Research, 2022, v. 152, n. 2, p. 193, doi. 10.1007/s11120-022-00920-z

By:

Zhu, Qingjun;
Yang, Yanyan;
Xiao, Yanan;
Han, Wenhui;
Li, Xingyue;
Wang, Wenda;
Kuang, Tingyun;
Shen, Jian-Ren;
Han, Guangye

Publication type:

Article

The structural basis of far-red light absorbance by allophycocyanins.

Published in:

Photosynthesis Research, 2021, v. 147, n. 1, p. 11, doi. 10.1007/s11120-020-00787-y

By:

Soulier, Nathan;
Bryant, Donald A.

Publication type:

Article

Thermodynamics of ferredoxin binding to cyanobacterial nitrate reductase.

Published in:

Photosynthesis Research, 2020, v. 144, n. 1, p. 73, doi. 10.1007/s11120-020-00738-7

By:

Srivastava, Anurag P.;
Mishra, Neelam;
Prasad, Ramachandra L. A.;
Rajesh, Preethi;
Knaff, David B.

Publication type:

Article

Dissecting the individual contribution of conserved cysteines to the redox regulation of RubisCO.

Published in:

Photosynthesis Research, 2018, v. 137, n. 2, p. 251, doi. 10.1007/s11120-018-0497-9

By:

García-Murria, María Jesús;
Sudhani, Hemanth P. K.;
Marín-Navarro, Julia;
Sánchez del Pino, Manuel M.;
Moreno, Joaquín

Publication type:

Article

Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride.

Published in:

Biotechnology Letters, 2021, v. 43, n. 8, p. 1585, doi. 10.1007/s10529-021-03109-x

By:

Yawata, Satoshi;
Noda, Kenichi;
Shimomura, Ai;
Kuroda, Akio

Publication type:

Article

A single point mutation converts a glutaryl-7-aminocephalosporanic acid acylase into an N-acyl-homoserine lactone acylase.

Published in:

Biotechnology Letters, 2021, v. 43, n. 7, p. 1467, doi. 10.1007/s10529-021-03135-9

By:

Murugayah, Shereen A.;
Evans, Gary B.;
Tyndall, Joel D. A.;
Gerth, Monica L.

Publication type:

Article

Surface charge engineering of Thermomyces lanuginosus lipase improves enzymatic activity and biodiesel synthesis.

Published in:

Biotechnology Letters, 2021, v. 43, n. 7, p. 1403, doi. 10.1007/s10529-021-03126-w

By:

Han, Nanyu;
Tang, Minyuan;
Wan, Sidi;
Jiang, Zhanbao;
Yue, Yong;
Zhao, Xiangui;
Yang, Jinrun;
Huang, Zunxi

Publication type:

Article

Computer-aided engineering of adipyl-CoA synthetase for enhancing adipic acid synthesis.

Published in:

Biotechnology Letters, 2020, v. 42, n. 12, p. 2693, doi. 10.1007/s10529-020-02978-y

By:

Yang, Ju;
Wei, Yafeng;
Li, Guohui;
Zhou, Shenghu;
Deng, Yu

Publication type:

Article

Inulin catabolism in Saccharomyces cerevisiae is affected by some key glycosylation sequons of invertase Suc2.

Published in:

Biotechnology Letters, 2020, v. 42, n. 3, p. 471, doi. 10.1007/s10529-020-02791-7

By:

Yang, Fan;
Zhang, Xinyu;
Lu, Yuting;
Wang, Biying;
Chen, Xiaoyi;
Sun, Zhen;
Li, Xianzhen

Publication type:

Article

Stabilization of fungi-derived recombinant FAD-dependent glucose dehydrogenase by introducing a disulfide bond.

Published in:

Biotechnology Letters, 2015, v. 37, n. 5, p. 1091, doi. 10.1007/s10529-015-1774-8

By:

Sakai, Genki;
Kojima, Katsuhiro;
Mori, Kazushige;
Oonishi, Yosuke;
Sode, Koji

Publication type:

Article

Stabilization of human immunodeficiency virus type 1 reverse transcriptase by site-directed mutagenesis.

Published in:

Biotechnology Letters, 2013, v. 35, n. 12, p. 2165, doi. 10.1007/s10529-013-1321-4

By:

Nishimura, Kosaku;
Shinomura, Mayu;
Konishi, Atsushi;
Yasukawa, Kiyoshi

Publication type:

Article

Site-directed mutagenesis in bacteriorhodopsin mutants and their characterization for bioelectrical and biotechnological equipment.

Published in:

Biotechnology Letters, 2012, v. 34, n. 3, p. 455, doi. 10.1007/s10529-011-0731-4

By:

Saeedi, Pardis;
Moosaabadi, Jafar;
Sebtahmadi, Seyed;
Behmanesh, Mehrdad;
Mehrabadi, Jalil

Publication type:

Article

Analysis on the factors affecting start-up intensity in the upstream sequence of phycocyanin β subunit gene from Arthrospira platensis by site-directed mutagenesis.

Published in:

Biotechnology Letters, 2007, v. 29, n. 3, p. 459, doi. 10.1007/s10529-006-9266-5

By:

Nan Guo;
Xuecheng Zhang;
Yongzhong Lu;
Xiaojin Song

Publication type:

Article

Insights into the catalytic and regulatory mechanisms of dihydroflavonol 4-reductase, a key enzyme of anthocyanin synthesis in Zanthoxylum bungeanum.

Published in:

Tree Physiology, 2023, v. 43, n. 1, p. 169, doi. 10.1093/treephys/tpac101

By:

Aiguo, Zhao;
Ruiwen, Ding;
Cheng, Wang;
Cheng, Chen;
Dongmei, Wang

Publication type:

Article

Role of conserved active site tryptophan-101 in functional activity and stability of phosphoserine aminotransferase from an enteric human parasite.

Published in:

Amino Acids, 2012, v. 43, n. 1, p. 483, doi. 10.1007/s00726-011-1105-x

By:

Mishra, Vibhor;
Kumar, Ashutosh;
Ali, Vahab;
Nozaki, Tomoyoshi;
Zhang, Kam;
Bhakuni, Vinod

Publication type:

Article

Site-directed mutagenesis and feedback-resistant N-acetyl- L-glutamate kinase (NAGK) increase Corynebacterium crenatum L-arginine production.

Published in:

Amino Acids, 2012, v. 43, n. 1, p. 255, doi. 10.1007/s00726-011-1069-x

By:

Xu, Meijuan;
Rao, Zhiming;
Dou, Wenfang;
Yang, Juan;
Jin, Jian;
Xu, Zhenghong

Publication type:

Article

Probing mammalian spermine oxidase enzyme-substrate complex through molecular modeling, site-directed mutagenesis and biochemical characterization.

Published in:

Amino Acids, 2011, v. 40, n. 4, p. 1115, doi. 10.1007/s00726-010-0735-8

By:

Tavladoraki, Paraskevi;
Cervelli, Manuela;
Antonangeli, Fabrizio;
Minervini, Giovanni;
Stano, Pasquale;
Federico, Rodolfo;
Mariottini, Paolo;
Polticelli, Fabio

Publication type:

Article

Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase.

Published in:

Amino Acids, 2009, v. 37, n. 3, p. 523, doi. 10.1007/s00726-009-0256-5

By:

Lu Sun;
Xin Wen;
Ying Tan;
Heyang Li;
Xing Yang;
Yuefang Zhao;
Baifan Wang;
Qiongyao Cao;
Congwei Niu;
Zhen Xi

Publication type:

Article

Engineering a feedback inhibition-insensitive plant dihydrodipicolinate synthase to increase lysine content in Camelina sativa seeds.

Published in:

Transgenic Research, 2022, v. 31, n. 1, p. 131, doi. 10.1007/s11248-021-00291-6

By:

Huang, Alex;
Coutu, Cathy;
Harrington, Myrtle;
Rozwadowski, Kevin;
Hegedus, Dwayne D.

Publication type:

Article

Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the T1 generation.

Published in:

Transgenic Research, 2021, v. 30, n. 1, p. 77, doi. 10.1007/s11248-020-00229-4

By:

Adachi, Kohei;
Hirose, Aya;
Kanazashi, Yuhei;
Hibara, Miki;
Hirata, Toshiyuki;
Mikami, Masafumi;
Endo, Masaki;
Hirose, Sakiko;
Maruyama, Nobuyuki;
Ishimoto, Masao;
Abe, Jun;
Yamada, Tetsuya

Publication type:

Article

Target-specific mutations efficiency at multiple loci of CRISPR/Cas9 system using one sgRNA in soybean.

Published in:

Transgenic Research, 2021, v. 30, n. 1, p. 51, doi. 10.1007/s11248-020-00228-5

By:

Zhang, Ling;
Wang, Yingzhe;
Li, Tong;
Qiu, Hongmei;
Xia, Zhengjun;
Dong, Yingshan

Publication type:

Article

Dynamics of loops surrounding the active site architecture in GH5_2 subfamily TfCel5A for cellulose degradation.

Published in:

Biotechnology for Biofuels & Bioproducts, 2023, v. 16, n. 1, p. 1, doi. 10.1186/s13068-023-02411-2

By:

Wu, Xiuyun;
Zhao, Sha;
Tian, Zhennan;
Han, Chao;
Jiang, Xukai;
Wang, Lushan

Publication type:

Article

Identification of critical residues of O-antigen-modifying O-acetyltransferase B (OacB) of Shigella flexneri.

Published in:

BMC Molecular & Cell Biology, 2022, v. 23, n. 1, p. 1, doi. 10.1186/s12860-022-00415-8

By:

Rajput, Munazza I.;
Verma, Naresh K.

Publication type:

Article

Revealing the key point of the temperature stress response of Arthrospira platensis C1 at the interconnection of C- and N- metabolism by proteome analyses and PPI networking.

Published in:

BMC Molecular & Cell Biology, 2020, v. 21, n. 1, p. 1, doi. 10.1186/s12860-020-00285-y

By:

Kurdrid, Pavinee;
Phuengcharoen, Phutnichar;
Senachak, Jittisak;
Saree, Sirilak;
Hongsthong, Apiradee

Publication type:

Article

Risk assessment and molecular mechanism of Fusarium incarnatum resistance to phenamacril.

Published in:

Pest Management Science, 2022, v. 78, n. 8, p. 3394, doi. 10.1002/ps.6967

By:

Mao, Yushuai;
Zhao, Baoquan;
Cao, Zhiguo;
Shen, Jinghan;
Xu, Shaohua;
Wu, Jian;
Li, Tao;
Wang, Jianxin;
Statsyuk, Natalia;
Shcherbakova, Larisa;
Zhou, Mingguo;
Duan, Yabing

Publication type:

Article

CRISPR/Cas9 mediates efficient site‐specific mutagenesis of the odorant receptor co‐receptor (Orco) in the malaria vector Anopheles sinensis.

Published in:

Pest Management Science, 2022, v. 78, n. 8, p. 3294, doi. 10.1002/ps.6954

By:

Wang, You;
He, Xingfei;
Qiao, Liang;
Yu, Zhengrong;
Chen, Bin;
He, Zhengbo

Publication type:

Article

Fenoxaprop‐P‐ethyl resistance conferred by cytochrome P450s and target site mutation in Alopecurus japonicus.

Published in:

Pest Management Science, 2018, v. 74, n. 7, p. 1694, doi. 10.1002/ps.4863

By:

Chen, Guoqi;
Zhang, Teng;
Bai, Congqiang;
Dong, Liyao;
Xu, Hongle

Publication type:

Article

The Y137H mutation in the cytochrome P450 FgCYP51B protein confers reduced sensitivity to tebuconazole in Fusarium graminearum.

Published in:

Pest Management Science, 2018, v. 74, n. 6, p. 1472, doi. 10.1002/ps.4837

By:

Qian, Hengwei;
Chi, Mengyu;
Liang, Wenxing;
Huang, Jinguang;
Li, Baodu;
Du, Juan;
Sun, Xiaomei

Publication type:

Article

Influence of Phosphorylation of THR-3, SER-11, and SER-15 on Deoxycytidine Kinase Activity and Stability.

Published in:

Nucleosides, Nucleotides & Nucleic Acids, 2010, v. 29, n. 4-6, p. 404, doi. 10.1080/15257771003741216

By:

Smal, C.;
Ntamashimikiro, S.;
Arts, A.;
Van Den Neste, E.;
Bontemps, F.

Publication type:

Article

Improved efficacy of soluble human receptor activator of nuclear factor kappa B (RANK) fusion protein by site-directed mutagenesis.

Published in:

Immunopharmacology & Immunotoxicology, 2015, v. 37, n. 3, p. 221, doi. 10.3109/08923973.2015.1035392

By:

Son, Young Jun;
Han, Jihye;
Lee, Jae Yeon;
Kim, HaHyung;
Chun, Taehoon

Publication type:

Article

嗜热毛壳菌多糖单加氧酶的氧化特性及协同作用.

Published in:

Mycosystema, 2022, v. 41, n. 7, p. 1068, doi. 10.13346/j.mycosystema.210363

By:

夏东慧;
刘宁;
郭秀娜;
李多川

Publication type:

Article

苹果黑腐皮壳菌CAP 超家族蛋白VmPR1c 的降解功能域和降解途径.

Published in:

Mycosystema, 2019, v. 38, n. 9, p. 1470, doi. 10.13346/j.mycosystema.190059

By:

孟香;
尹志远;
聂嘉俊;
黄丽丽

Publication type:

Article

Problem-solving test: The role of ubiquitination in epidermal growth factor receptor trafficking.

Published in:

Biochemistry & Molecular Biology Education, 2012, v. 40, n. 4, p. 273, doi. 10.1002/bmb.20616

By:

Szeberényi, József

Publication type:

Article

The cysteine 34 residue of A1M/α<sub>1</sub>-microglobulin is essential for protection of irradiated cell cultures and reduction of carbonyl groups.

Published in:

Free Radical Research, 2013, v. 47, n. 6/7, p. 541, doi. 10.3109/10715762.2013.801555

By:

Rutardottir, S.;
Nilsson, E. J. C.;
Pallon, J.;
Gram, M.;
Åkerström, B.

Publication type:

Article

Site-Directed Mutations at Phosphorylation Sites in Zea mays PHO1 Reveal Modulation of Enzymatic Activity by Phosphorylation at S566 in the L80 Region.

Published in:

Plants (2223-7747), 2023, v. 12, n. 18, p. 3205, doi. 10.3390/plants12183205

By:

Shoaib, Noman;
Mughal, Nishbah;
Liu, Lun;
Raza, Ali;
Shen, Leiyang;
Yu, Guowu

Publication type:

Article

N-Linked Glycosylation Modulates Golgi-Independent Vacuolar Sorting Mediated by the Plant Specific Insert.

Published in:

Plants (2223-7747), 2019, v. 8, n. 9, p. 312, doi. 10.3390/plants8090312

By:

Vieira, Vanessa;
Peixoto, Bruno;
Costa, Mónica;
Pereira, Susana;
Pissarra, José;
Pereira, Cláudia

Publication type:

Article

Salt-tolerant and thermostable mechanisms of an endoglucanase from marine Aspergillus niger.

Published in:

Bioresources & Bioprocessing, 2022, v. 9, n. 1, p. 1, doi. 10.1186/s40643-022-00533-3

By:

Cai, Li-Nian;
Xu, Sheng-Nan;
Lu, Tao;
Lin, Dong-Qiang;
Yao, Shan-Jing

Publication type:

Article

Tailoring pullulanase PulAR from Anoxybacillus sp. AR-29 for enhanced catalytic performance by a structure-guided consensus approach.

Published in:

Bioresources & Bioprocessing, 2022, v. 9, n. 1, p. 1, doi. 10.1186/s40643-022-00516-4

By:

Li, Shu-Fang;
Xu, Shen-Yuan;
Wang, Ya-Jun;
Zheng, Yu-Guo

Publication type:

Article

Enhancing the thermostability and activity of uronate dehydrogenase from Agrobacterium tumefaciens LBA4404 by semi-rational engineering.

Published in:

Bioresources & Bioprocessing, 2019, v. 6, n. 1, p. N.PAG, doi. 10.1186/s40643-019-0267-3

By:

Su, Hui-Hui;
Peng, Fei;
Xu, Pei;
Wu, Xiao-Ling;
Zong, Min-Hua;
Yang, Ji-Guo;
Lou, Wen-Yong

Publication type:

Article

A novel synonymous KMT2B variant in a patient with dystonia causes aberrant splicing.

Published in:

Molecular Genetics & Genomic Medicine, 2022, v. 10, n. 5, p. 1, doi. 10.1002/mgg3.1923

By:

Grosz, Bianca R.;
Tisch, Stephen;
Tchan, Michel C.;
Fung, Victor S. C.;
Darveniza, Paul;
Fellner, Avi;
Kurian, Manju A.;
McLean, Alison;
Tomlinson, Susan E.;
Smyth, Renee;
Devery, Sophie;
Wu, Kathy H. C.;
Kennerson, Marina L.;
Kumar, Kishore R.

Publication type:

Article

Functional evaluation of a novel GLA causative mutation in Fabry disease.

Published in:

Molecular Genetics & Genomic Medicine, 2019, v. 7, n. 9, p. N.PAG, doi. 10.1002/mgg3.864

By:

Li, Ping;
Zhang, Lijuan;
Xiong, Qiuhong;
Wang, Zhe;
Cui, Xiaodong;
Zhou, Yong‐An;
Wang, Yuxian;
Xiao, Han;
Wu, Changxin

Publication type:

Article