Works matching DE "SITE-specific mutagenesis"

Results: 1393

Cobalt Chloride–Mimicked Hepatocyte Cell Hypoxia Induces TREX1, Leading to Hepatitis B Virus Restriction.

Published in:

Journal of Infectious Diseases, 2025, v. 231, n. 6, p. 1559, doi. 10.1093/infdis/jiaf002

By:

Suspène, Rodolphe;
Caval, Vincent;
Khalfi, Pierre;
Pitré, Emmanuelle;
Marchio, Agnès;
Pineau, Pascal;
Vartanian, Jean-Pierre

Publication type:

Article

The Structural Deciphering of the α3 Helix Within ZmHsfA2'S DNA-Binding Domain for the Recognition of Heat Shock Elements in Maize.

Published in:

Plants (2223-7747), 2025, v. 14, n. 13, p. 1950, doi. 10.3390/plants14131950

By:

Wang, Yantao;
Ma, Zhenyu;
Li, Guoliang;
Meng, Xiangzhao;
Duan, Shuonan;
Liu, Zihui;
Zhao, Min;
Guo, Xiulin;
Zhang, Huaning

Publication type:

Article

Reduction in Cold-Induced Sweetening by Cas9 Endonuclease-Mediated Knockout of the POTATO VACUOLAR INVERTASE 1 Gene in the Cultivar 'Symfonia'.

Published in:

Potato Research, 2025, v. 68, n. 2, p. 1571, doi. 10.1007/s11540-024-09800-6

By:

Egorova, Anastasiya A.;
Zykova, Tatyana E.;
Kostina, Nina E.;
Saboiev, Izatsho A.;
Koloshina, Kristina A.;
Filipenko, Elena A.;
Hoffie, Iris;
Hertig, Christian;
Hiekel, Stefan;
Kumlehn, Jochen;
Kochetov, Alex V.;
Gerasimova, Sophia V.

Publication type:

Article

Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.

Published in:

Frontiers in Zoology, 2025, v. 22, n. 1, p. 1, doi. 10.1186/s12983-025-00566-2

By:

Ahmed, Hassan M. M.;
Zheng, Lisha;
Hunnekuhl, Vera S.

Publication type:

Article

Site-Directed Mutagenesis Mediated by Molecular Modeling and Docking and Its Effect on the Protein–Protein Interactions of the bHLH Transcription Factors SPATULA, HECATE1, and INDEHISCENT.

Published in:

Plants (2223-7747), 2025, v. 14, n. 12, p. 1756, doi. 10.3390/plants14121756

By:

López-Gómez, Pablo;
De La Mora-Franco, Daniela;
Herrera-Ubaldo, Humberto;
Díaz-Quezada, Corina;
Brieba, Luis G.;
de Folter, Stefan

Publication type:

Article

Multiple Mechanisms of HIV-1 Resistance to PGT135 in a Chinese Subtype B' Slow Progressor.

Published in:

Pathogens, 2025, v. 14, n. 6, p. 556, doi. 10.3390/pathogens14060556

By:

Hu, Yuanyuan;
Sun, Shasha;
Liu, Ying;
Ren, Li;
Hu, Xintao;
Ruan, Yuhua;
Ma, Liying;
Liang, Hao;
Shao, Yiming;
Hong, Kunxue;
Zou, Sen;
Hao, Yanling

Publication type:

Article

FpFumB Is Required for Basic Biological Processes and Virulence in Fusarium proliferatum by Modulating DNA Repair Through Interaction with FpSae2.

Published in:

Microorganisms, 2025, v. 13, n. 6, p. 1433, doi. 10.3390/microorganisms13061433

By:

Gao, Yizhou;
Li, Haibo;
Liu, Yong;
Wang, Yuqing;
Xue, Jingwen;
Wang, Yitong;
Wu, Zhihong

Publication type:

Article

Synergistic Engineering of the Twin-Arginine Translocation (Tat) Pathway and Membrane Capacity Enhances Extracellular Production of Amylosucrase in Bacillus licheniformis.

Published in:

Microorganisms, 2025, v. 13, n. 6, p. 1179, doi. 10.3390/microorganisms13061179

By:

Wang, Caizhe;
Niu, Dandan;
Zhou, Yongqing;
Liu, Hui;
Mchunu, Nokuthula Peace;
Zhang, Meng;
Singh, Suren;
Wang, Zhengxiang

Publication type:

Article

Engineering Sanghuangporus sanghuang for enhanced (-)-aristolone production via metabolic pathway optimization and terpene synthase engineering.

Published in:

Applied Microbiology & Biotechnology, 2025, v. 109, n. 1, p. 1, doi. 10.1007/s00253-025-13540-z

By:

Li, Yihan;
Kang, Chuanzhi;
Xu, Jiahui;
Zhou, Wenqing;
Pan, Weishan;
Xia, Daofang;
Liang, Jian;
Guo, Lanping;
Ma, Xiao-kui

Publication type:

Article

Cytochrome P450 CYP76F14 Mediates the Conversion of Its Substrate Linalool in Table Grape Berries.

Published in:

Horticulturae, 2025, v. 11, n. 6, p. 651, doi. 10.3390/horticulturae11060651

By:

Song, Zhizhong;
Zhang, Jinjin;
Shi, Matthew;
Li, Dong;
Liu, Xiaohua

Publication type:

Article

Functional Identification of Catalytic Metal Ion Binding Sites within RNA.

Published in:

PLoS Biology, 2005, v. 3, n. 8, p. 1, doi. 10.1371/journal.pbio.0030277

By:

Hougland, James L;
Kravchuk, Alexander V;
Herschlag, Daniel;
Piccirilli, Joseph A

Publication type:

Article

Mechanism of Association and Reciprocal Activation of Two GTPases.

Published in:

PLoS Biology, 2004, v. 2, n. 9, p. 1, doi. 10.1371/journal.pbio.0020320

By:

Shan, Shu-ou;
Stroud, Robert M;
Walter, Peter

Publication type:

Article

HBV clinical isolates expressing adefovir resistance mutations show similar tenofovir susceptibilities across genotypes B, C and D.

Published in:

Liver International, 2014, v. 34, n. 7, p. 1025, doi. 10.1111/liv.12343

By:

Liu, Yang;
Miller, Michael D.;
Kitrinos, Kathryn M.

Publication type:

Article

Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus.

Published in:

Photosynthesis Research, 2022, v. 152, n. 2, p. 193, doi. 10.1007/s11120-022-00920-z

By:

Zhu, Qingjun;
Yang, Yanyan;
Xiao, Yanan;
Han, Wenhui;
Li, Xingyue;
Wang, Wenda;
Kuang, Tingyun;
Shen, Jian-Ren;
Han, Guangye

Publication type:

Article

The structural basis of far-red light absorbance by allophycocyanins.

Published in:

Photosynthesis Research, 2021, v. 147, n. 1, p. 11, doi. 10.1007/s11120-020-00787-y

By:

Soulier, Nathan;
Bryant, Donald A.

Publication type:

Article

Thermodynamics of ferredoxin binding to cyanobacterial nitrate reductase.

Published in:

Photosynthesis Research, 2020, v. 144, n. 1, p. 73, doi. 10.1007/s11120-020-00738-7

By:

Srivastava, Anurag P.;
Mishra, Neelam;
Prasad, Ramachandra L. A.;
Rajesh, Preethi;
Knaff, David B.

Publication type:

Article

Dissecting the individual contribution of conserved cysteines to the redox regulation of RubisCO.

Published in:

Photosynthesis Research, 2018, v. 137, n. 2, p. 251, doi. 10.1007/s11120-018-0497-9

By:

García-Murria, María Jesús;
Sudhani, Hemanth P. K.;
Marín-Navarro, Julia;
Sánchez del Pino, Manuel M.;
Moreno, Joaquín

Publication type:

Article

Microcalorimetric study on the transcription start site mutagenesis.

Published in:

Journal of Thermal Analysis & Calorimetry, 2004, v. 75, n. 1, p. 293, doi. 10.1023/B:JTAN.0000017350.19290.35

By:

Yang, Y.;
Liu, Y.;
Zhu, J.;
Shen, P.

Publication type:

Article

Essential amino acid residues and catalytic mechanism of trans-epoxysuccinate hydrolase for production of meso-tartaric acid.

Published in:

Biotechnology Letters, 2024, v. 46, n. 5, p. 739, doi. 10.1007/s10529-024-03490-3

By:

Liao, Hongxiu;
Pan, Haifeng;
Yao, Jinfeng;
Zhu, Ronglin;
Bao, Wenna

Publication type:

Article

Enhanced enzyme thermostability of a family I.3 lipase LipSR1 by T118A mutation at the calcium-binding site.

Published in:

Biotechnology Letters, 2023, v. 45, n. 9, p. 1199, doi. 10.1007/s10529-023-03413-8

By:

Jiang, Shijie;
Zhou, Zhengfu;
Han, Jiahui;
Fan, Qingfeng;
Long, Zhijian;
Wang, Jin

Publication type:

Article

Thermostability improvement of sucrose isomerase PalI NX-5: a comprehensive strategy.

Published in:

Biotechnology Letters, 2023, v. 45, n. 7, p. 885, doi. 10.1007/s10529-023-03388-6

By:

Sardiña-Peña, A. J.;
Ballinas-Casarrubias, L.;
Siqueiros-Cendón, T. S.;
Espinoza-Sánchez, E. A.;
Flores-Holguín, N. R.;
Iglesias-Figueroa, B. F.;
Rascón-Cruz, Q.

Publication type:

Article

CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes.

Published in:

Biotechnology Letters, 2023, v. 45, n. 5/6, p. 629, doi. 10.1007/s10529-023-03363-1

By:

Zhang, Guoyi;
Wang, Junyu;
Li, Yiwen;
Shang, Guangdong

Publication type:

Article

Improvement of the catalytic performance of glycerol kinase from Bacillus subtilis by chromosomal site-directed mutagenesis.

Published in:

Biotechnology Letters, 2022, v. 44, n. 9, p. 1051, doi. 10.1007/s10529-022-03281-8

By:

Wang, Guanglu;
Wang, Mengyuan;
Liu, Lanxi;
Hui, Xiaohan;
Wang, Bingyang;
Ma, Ke;
Yang, Xuepeng

Publication type:

Article

Mutant firefly luciferase enzymes resistant to the inhibition by sodium chloride.

Published in:

Biotechnology Letters, 2021, v. 43, n. 8, p. 1585, doi. 10.1007/s10529-021-03109-x

By:

Yawata, Satoshi;
Noda, Kenichi;
Shimomura, Ai;
Kuroda, Akio

Publication type:

Article

A single point mutation converts a glutaryl-7-aminocephalosporanic acid acylase into an N-acyl-homoserine lactone acylase.

Published in:

Biotechnology Letters, 2021, v. 43, n. 7, p. 1467, doi. 10.1007/s10529-021-03135-9

By:

Murugayah, Shereen A.;
Evans, Gary B.;
Tyndall, Joel D. A.;
Gerth, Monica L.

Publication type:

Article

Surface charge engineering of Thermomyces lanuginosus lipase improves enzymatic activity and biodiesel synthesis.

Published in:

Biotechnology Letters, 2021, v. 43, n. 7, p. 1403, doi. 10.1007/s10529-021-03126-w

By:

Han, Nanyu;
Tang, Minyuan;
Wan, Sidi;
Jiang, Zhanbao;
Yue, Yong;
Zhao, Xiangui;
Yang, Jinrun;
Huang, Zunxi

Publication type:

Article

Computer-aided engineering of adipyl-CoA synthetase for enhancing adipic acid synthesis.

Published in:

Biotechnology Letters, 2020, v. 42, n. 12, p. 2693, doi. 10.1007/s10529-020-02978-y

By:

Yang, Ju;
Wei, Yafeng;
Li, Guohui;
Zhou, Shenghu;
Deng, Yu

Publication type:

Article

Inulin catabolism in Saccharomyces cerevisiae is affected by some key glycosylation sequons of invertase Suc2.

Published in:

Biotechnology Letters, 2020, v. 42, n. 3, p. 471, doi. 10.1007/s10529-020-02791-7

By:

Yang, Fan;
Zhang, Xinyu;
Lu, Yuting;
Wang, Biying;
Chen, Xiaoyi;
Sun, Zhen;
Li, Xianzhen

Publication type:

Article

Phosphorylation of BCL2 at the Ser70 site mediates RANKL-induced osteoclast precursor autophagy and osteoclastogenesis.

Published in:

Molecular Medicine, 2022, v. 28, n. 1, p. 1, doi. 10.1186/s10020-022-00449-w

By:

Ke, Dianshan;
Yu, Yunlong;
Li, Chenglong;
Han, Junyong;
Xu, Jie

Publication type:

Article

CcCHIL, a type IV chaicone isomerase that can improve (2S)-naringenin production in Saccharomyces cerevisiae.

Published in:

Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2024, v. 148, p. 229, doi. 10.1016/j.fbp.2024.09.016

By:

Sisheng Zhang;
Jianmu Su;
Tsan-Yu Chiu;
Jintao Fang;
Xiangxiu Liang;
Zhuoyuan He;
Shancen Zhao;
Hong Wu

Publication type:

Article

Activity enhancement of 1¥ametes versicolor aflatoxin Bl-degrading enzyme (TV-AFBlD) by molecular docking and site-directed mutagenesis techniques.

Published in:

Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2021, v. 129, p. 168, doi. 10.1016/j.fbp.2021.08.007

By:

Peizhou Yanga;
Shuhua Lu;
Wei Xiao;
Zhi Zheng;
Suwei Jiang;
Shaotong Jiang;
Shuying Jiang;
Jieshun Cheng;
Danfeng Zhang

Publication type:

Article

Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.

Published in:

Current Genetics, 2023, v. 69, n. 2/3, p. 127, doi. 10.1007/s00294-023-01262-6

By:

Lettow, Julia;
Kliewe, Felix;
Aref, Rasha;
Schüller, Hans-Joachim

Publication type:

Article

Transcriptional repressor Gal80 recruits corepressor complex Cyc8–Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.

Published in:

Current Genetics, 2022, v. 68, n. 1, p. 115, doi. 10.1007/s00294-021-01215-x

By:

Lettow, Julia;
Aref, Rasha;
Schüller, Hans-Joachim

Publication type:

Article

Phosphorylation by Prp4 kinase releases the self-inhibition of FgPrp31 in Fusarium graminearum.

Published in:

Current Genetics, 2018, v. 64, n. 6, p. 1261, doi. 10.1007/s00294-018-0838-4

By:

Gao, Xuli;
Zhang, Ju;
Song, Chaoni;
Yuan, Kangyi;
Wang, Jianhua;
Jin, Qiaojun;
Xu, Jin-Rong

Publication type:

Article

Cloning, expression, and characterization of a four-component O-demethylase from human intestinal bacterium Eubacterium limosum ZL-II.

Published in:

Applied Microbiology & Biotechnology, 2016, v. 100, n. 21, p. 9111, doi. 10.1007/s00253-016-7626-1

By:

Chen, Jia-Xing;
Deng, Chao-Yin;
Zhang, Ying-Tao;
Liu, Zhen-Ming;
Wang, Ping-Zhang;
Liu, Shu-Lin;
Qian, Wei;
Yang, Dong-Hui

Publication type:

Article

Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli.

Published in:

Applied Microbiology & Biotechnology, 2016, v. 100, n. 11, p. 4901, doi. 10.1007/s00253-015-7272-z

By:

Tong, Wenhua;
Xu, Ying;
Xian, Mo;
Niu, Wei;
Guo, Jiantao;
Liu, Huizhou;
Zhao, Guang

Publication type:

Article

Structural insights into methanol-stable variants of lipase T6 from Geobacillus stearothermophilus.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 22, p. 9449, doi. 10.1007/s00253-015-6700-4

By:

Dror, Adi;
Kanteev, Margarita;
Kagan, Irit;
Gihaz, Shalev;
Shahar, Anat;
Fishman, Ayelet

Publication type:

Article

Improving the catalytic performance of a GH11 xylanase by rational protein engineering.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 22, p. 9503, doi. 10.1007/s00253-015-6712-0

By:

Cheng, Ya-Shan;
Chen, Chun-Chi;
Huang, Jian-Wen;
Ko, Tzu-Ping;
Huang, Zhiyong;
Guo, Rey-Ting

Publication type:

Article

Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 22, p. 9473, doi. 10.1007/s00253-015-6717-8

By:

Lee, Hyunji;
Park, Jiyoung;
Jung, Chaewon;
Han, Dongfei;
Seo, Jiyoung;
Ahn, Joong-Hoon;
Chong, Youhoon;
Hur, Hor-Gil

Publication type:

Article

Mutational analysis to identify the residues essential for the inhibition of N-acetyl glutamate kinase of Corynebacterium glutamicum.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 18, p. 7527, doi. 10.1007/s00253-015-6469-5

By:

Huang, Yuanyuan;
Zhang, Hao;
Tian, Hongming;
Li, Cheng;
Han, Shuangyan;
Lin, Ying;
Zheng, Suiping

Publication type:

Article

Archaeal DNA polymerases in biotechnology.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 16, p. 6585, doi. 10.1007/s00253-015-6781-0

By:

Zhang, Likui;
Kang, Manyu;
Xu, Jiajun;
Huang, Yanchao

Publication type:

Article

Thermostability enhancement of an endo-1,4-β-galactanase from Talaromyces stipitatus by site-directed mutagenesis.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 10, p. 4245, doi. 10.1007/s00253-014-6244-z

By:

Larsen, Dorte;
Nyffenegger, Christian;
Swiniarska, Maria;
Thygesen, Anders;
Strube, Mikael;
Meyer, Anne;
Mikkelsen, Jørn

Publication type:

Article

Improvement of the amides forming capacity of the arylacetonitrilase from Pseudomonas fluorescens EBC191 by site-directed mutagenesis.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 6, p. 2623, doi. 10.1007/s00253-014-6061-4

By:

Sosedov, Olga;
Stolz, Andreas

Publication type:

Article

Isolation of a Bluetongue virus group-specific monoclonal antibody and application to a diagnostic competitive ELISA.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 2, p. 729, doi. 10.1007/s00253-014-5937-7

By:

Xu, Q.;
Sun, E.;
Yang, T.;
Feng, Y.;
Geng, H.;
Wei, T.;
Li, J.;
Sun, L.;
Sun, J.;
Xi, N.;
Zhang, Q.;
Wang, H.;
Liu, E.;
Bu, Z.;
Wu, D.

Publication type:

Article

Effects of mutation at position 285 of Ralstonia pickettii T1 poly[( R)-3-hydroxybutyrate] depolymerase on its activities.

Published in:

Applied Microbiology & Biotechnology, 2014, v. 98, n. 16, p. 7061, doi. 10.1007/s00253-014-5660-4

By:

Tan, Liu-Tzea;
Hiraishi, Tomohiro;
Sudesh, Kumar;
Maeda, Mizuo

Publication type:

Article

Role of surface residue 184 in the catalytic activity of NADH oxidase from Streptococcus pyogenes.

Published in:

Applied Microbiology & Biotechnology, 2014, v. 98, n. 16, p. 7081, doi. 10.1007/s00253-014-5666-y

By:

Gao, Hui;
Tiwari, Manish;
Singh, Raushan;
Sung, Bong;
Kim, Sun;
Lee, Jung-Kul

Publication type:

Article

Characterization of propionate CoA-transferase from Ralstonia eutropha H16.

Published in:

Applied Microbiology & Biotechnology, 2014, v. 98, n. 8, p. 3579, doi. 10.1007/s00253-013-5222-1

By:

Volodina, Elena;
Schürmann, Marc;
Lindenkamp, Nicole;
Steinbüchel, Alexander

Publication type:

Article

Molecular engineering of industrial enzymes: recent advances and future prospects.

Published in:

Applied Microbiology & Biotechnology, 2014, v. 98, n. 1, p. 23, doi. 10.1007/s00253-013-5370-3

By:

Yang, Haiquan;
Li, Jianghua;
Shin, Hyun-dong;
Du, Guocheng;
Liu, Long;
Chen, Jian

Publication type:

Article

The key role of a non-active-site residue Met148 on the catalytic efficiency of meta-cleavage product hydrolase BphD.

Published in:

Applied Microbiology & Biotechnology, 2013, v. 97, n. 24, p. 10399, doi. 10.1007/s00253-013-4814-0

By:

Zhou, Hao;
Qu, Yuanyuan;
Kong, Chunlei;
Shen, E.;
Wang, Jingwei;
Zhang, Xuwang;
Ma, Qiao;
Zhou, Jiti

Publication type:

Article

Identification of critical residues for the activity and thermostability of Streptomyces sp. SK glucose isomerase.

Published in:

Applied Microbiology & Biotechnology, 2013, v. 97, n. 22, p. 9715, doi. 10.1007/s00253-013-4784-2

By:

Ben Hlima, Hajer;
Bejar, Samir;
Riguet, Jonas;
Haser, Richard;
Aghajari, Nushin

Publication type:

Article