Works matching Cloning

Results: 5000

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris.

Published in:

Microbial Cell Factories, 2015, v. 14, n. 1, p. 1, doi. 10.1186/s12934-015-0293-6

By:

Vogl, Thomas;
Ahmad, Mudassar;
Krainer, Florian W.;
Schwab, Helmut;
Glieder, Anton

Publication type:

Article

الإستنساخ أنواعه وأحكامه.

Published in:

Rihan Journal for Science Publishing, 2022, n. 23, p. 308

By:

شمس الاسلام شمس صافی

Publication type:

Article

In- & Out-Cloning: plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly.

Published in:

Synthetic Biology (23977000), 2024, v. 9, n. 1, p. 1, doi. 10.1093/synbio/ysae016

By:

Vries, Stijn T de;
Köbel, Tania S;
Sanal, Ahmet;
Schindler, Daniel

Publication type:

Article

Analysis of an epigenetic argument against human reproductive cloning.

Published in:

Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2006, v. 13, n. 2, p. 278, doi. 10.1016/S1472-6483(10)60627-X

By:

Nordgren, Anders

Publication type:

Article

IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation.

Published in:

PLoS ONE, 2014, v. 9, n. 9, p. 1, doi. 10.1371/journal.pone.0107907

By:

Wang, Jiancai;
Xu, Ronghua;
Liu, Aizhong

Publication type:

Article

TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.

Published in:

PLoS ONE, 2017, v. 12, n. 11, p. 1, doi. 10.1371/journal.pone.0186568

By:

Niarchos, Athanasios;
Siora, Anastasia;
Konstantinou, Evangelia;
Kalampoki, Vasiliki;
Lagoumintzis, George;
Poulas, Konstantinos

Publication type:

Article

Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method.

Published in:

PeerJ, 2018, p. 1, doi. 10.7717/peerj.5146

By:

Tan, Lendl;
Strong, Emily J.;
Woods, Kyra;
West, Nicholas P.

Publication type:

Article

Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis.

Published in:

Scientific Reports, 2024, v. 14, n. 1, p. 1, doi. 10.1038/s41598-024-72169-6

By:

Paclikova, Petra;
Harnos, Jakub

Publication type:

Article

Cloning and sequencing analysis of whole Spiroplasma genome in yeast.

Published in:

Frontiers in Microbiology, 2024, p. 1, doi. 10.3389/fmicb.2024.1411609

By:

Masaki Mizutani;
Sawako Omori;
Noriko Yamane;
Yo Suzuki;
Glass, John I.;
Ray-Yuan Chuang;
Takema Fukatsu;
Shigeyuki Kakizawa

Publication type:

Article

A strategy for in-house production of a positive selection cloning vector from the commercial pJET1.2/blunt cloning vector at minimal cost.

Published in:

3 Biotech, 2022, v. 12, n. 9, p. 1, doi. 10.1007/s13205-022-03289-x

By:

Nawawi, Omar;
Abdullah, Mohd Puad;
Yusuf, Chong Yu Lok

Publication type:

Article

Towards Practical Conservation Cloning: Understanding the Dichotomy Between the Histories of Commercial and Conservation Cloning.

Published in:

Animals (2076-2615), 2025, v. 15, n. 7, p. 989, doi. 10.3390/ani15070989

By:

Novak, Ben J.;
Brand, Stewart;
Phelan, Ryan;
Plichta, Sasha;
Ryder, Oliver A.;
Wiese, Robert J.

Publication type:

Article

Identifying the Public's Knowledge and Intention to Use Human Cloning in Greek Urban Areas.

Published in:

Journal of Assisted Reproduction & Genetics, 2005, v. 22, n. 2, p. 47, doi. 10.1007/s10815-005-1493-5

By:

Tzamalouka, Georgia;
Soultatou, Pelagia;
Papadakaki, Maria;
Chatzifotiou, Sevasti;
Tarlatzis, Basil;
Chliaoutakis, Joannes El.

Publication type:

Article

An Improved Homologous Recombination Method for Rapid Cloning of the Antibody Heavy Chain Gene and Its Comparison with the Homologous Recombination and Traditional Cloning Methods.

Published in:

Novelty in Biomedicine, 2015, n. 4, p. 171

By:

Hajirezaei, Masoumeh;
Darbouy, Mojtaba;
Rasouli, Manoochehr;
Kazemi, Bahram

Publication type:

Article

Escherichia coli BW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies.

Published in:

Frontiers in Microbiology, 2022, v. 13, p. 1, doi. 10.3389/fmicb.2022.838698

By:

Yang, Yuqing;
Yu, Qiaoli;
Wang, Min;
Zhao, Rui;
Liu, Huaiwei;
Xun, Luying;
Xia, Yongzhen

Publication type:

Article

pELMO, an optimised in-house cloning vector.

Published in:

AMB Express, 2017, v. 7, n. 1, p. 1, doi. 10.1186/s13568-017-0324-2

By:

Ramos, Andrea;
Muñoz, Marina;
Moreno-Pérez, Darwin;
Patarroyo, Manuel

Publication type:

Article

An efficient method for integration of PCR fragments into adjacent or overlapping restriction sites during gene cloning.

Published in:

3 Biotech, 2018, v. 8, n. 4, p. 1, doi. 10.1007/s13205-018-1214-2

By:

Makam, Shivakiran;
Srirama, Krupanidhi;
Dirisala, Vijaya Ramu;
Reddy, Prakash Narayana

Publication type:

Article

Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris.

Published in:

PLoS ONE, 2019, v. 14, n. 1, p. 1, doi. 10.1371/journal.pone.0211156

By:

Gräfe, Katharina;
Shanmugarajah, Kalpana;
Zobel, Thomas;
Weidtkamp-Peters, Stefanie;
Kleinschrodt, Diana;
Smits, Sander H. J.;
Schmitt, Lutz

Publication type:

Article

Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system.

Published in:

PLoS ONE, 2018, v. 13, n. 5, p. 1, doi. 10.1371/journal.pone.0197185

By:

Gantner, Johannes;
Ordon, Jana;
Ilse, Theresa;
Kretschmer, Carola;
Gruetzner, Ramona;
Löfke, Christian;
Dagdas, Yasin;
Bürstenbinder, Katharina;
Marillonnet, Sylvestre;
Stuttmann, Johannes

Publication type:

Article

A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement.

Published in:

Microbial Cell Factories, 2014, v. 13, n. 1, p. 1, doi. 10.1186/1475-2859-13-38

By:

Lund, Bjarte Aarmo;
Schrøder Leiros, Hanna-Kirsti;
Kjæreng Bjerga, Gro Elin

Publication type:

Article

A streamlined strategy for self-production of a commercial positive selection vector, the pJET1.2/blunt cloning vector, using common laboratory E. coli strains.

Published in:

3 Biotech, 2023, v. 13, n. 7, p. 1, doi. 10.1007/s13205-023-03647-3

By:

Nawawi, Omar;
Abdullah, Mohd Puad;
Yusuf, Chong Yu Lok

Publication type:

Article

Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning.

Published in:

PLoS ONE, 2014, v. 9, n. 3, p. 1, doi. 10.1371/journal.pone.0090896

By:

Tóth, Eszter;
Huszár, Krisztina;
Bencsura, Petra;
Kulcsár, Péter István;
Vodicska, Barbara;
Nyeste, Antal;
Welker, Zsombor;
Tóth, Szilvia;
Welker, Ervin

Publication type:

Article

An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from <i>Cupriavidus necator</i> in <i>Escherichia coli</i>.

Published in:

PLoS ONE, 2013, v. 8, n. 7, p. 1, doi. 10.1371/journal.pone.0068812

By:

Schiffels, Johannes;
Pinkenburg, Olaf;
Schelden, Maximilian;
Aboulnaga, El-Hussiny A. A.;
Baumann, Marcus E. M.;
Selmer, Thorsten

Publication type:

Article

Quantum Superimposing Multiple Anti-Cloning Machine.

Published in:

International Journal of Theoretical Physics, 2007, v. 46, n. 10, p. 2599, doi. 10.1007/s10773-007-9374-6

By:

Yan-ling Li;
Jian Feng;
Bao-long Liang;
Ya-fei Yu

Publication type:

Article

The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli.

Published in:

World Journal of Microbiology & Biotechnology, 2018, v. 34, n. 6, p. 1, doi. 10.1007/s11274-018-2466-z

By:

Rotchanapreeda, Tiwa;
Ngonsawan, Wasinee;
Klomtun, Manadsaree;
Somana, Jamorn

Publication type:

Article

THE NATURALNESS IN/OF THE ARTIFICIALITY OF CLONING.

Published in:

Euromentor, 2021, v. 12, n. 4, p. 90

By:

UZOMAH, M. MADUAWUCHI

Publication type:

Article

Handmade cloning: recent advances, potential and pitfalls.

Published in:

Journal of Animal Science & Biotechnology, 2015, v. 6, p. 1, doi. 10.1186/s40104-015-0043-y

By:

Verma, Geetika;
Arora, J. S.;
Sethi, R. S.;
Mukhopadhyay, C. S.;
Verma, Ramneek

Publication type:

Article

Asilomar, Gene Cloning's Origins, and Its Commercial Fate.

Published in:

Journal of the History of Biology, 2025, v. 58, n. 1, p. 21, doi. 10.1007/s10739-025-09803-0

By:

Yi, Doogab

Publication type:

Article

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning.

Published in:

BMC Biotechnology, 2013, v. 12, n. 1, p. 1, doi. 10.1186/1472-6750-13-12

By:

Scholz, Judith;
Besir, Hüseyin;
Strasser, Claudia;
Suppmann, Sabine

Publication type:

Article

Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning.

Published in:

Bioscience, Biotechnology & Biochemistry, 2024, v. 88, n. 7, p. 784, doi. 10.1093/bbb/zbae061

By:

Kuzuhara, Taiki;
Monden, Kota;
Hachiya, Takushi;
Nakagawa, Tsuyoshi

Publication type:

Article

Enhanced Cloning Efficiency of Differential Display PCR Amplicons.

Published in:

Plant Molecular Biology Reporter, 2004, v. 22, n. 3, p. 309, doi. 10.1007/BF02773144

By:

Sanabria, Natasha M.;
Dubery, Ian A.

Publication type:

Article

A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products.

Published in:

Biotechnology Letters, 2010, v. 32, n. 3, p. 439, doi. 10.1007/s10529-009-0166-3

By:

Qiong Wu;
Xing Zhong;
Chao Zhai;
Ju Yang;
Xiaomei Chen;
Liang Chen;
Wenfeng Wang;
Lixin Ma

Publication type:

Article

A simple and rapid strategy for the molecular cloning and monitoring of mouse HtrA2 serine protease.

Published in:

Biotechnology Letters, 2008, v. 30, n. 3, p. 397, doi. 10.1007/s10529-007-9556-6

By:

Goo-Young Kim;
Min-Kyung Nam;
Sang-Soo Kim;
Ho-Young Kim;
Sang-Kyu Lee;
Rhim, Hyangshuk

Publication type:

Article

Advances in molecular cloning.

Published in:

Molecular Biology, 2016, v. 50, n. 1, p. 1, doi. 10.1134/S0026893316010131

By:

Ashwini, Malla;
Murugan, Shanmugaraj;
Balamurugan, Srinivasan;
Sathishkumar, Ramalingam

Publication type:

Article

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR.

Published in:

PLoS ONE, 2017, v. 12, n. 8, p. 1, doi. 10.1371/journal.pone.0183974

By:

Huang, Faqing;
Spangler, Joseph Rankin;
Huang, Allen Yang

Publication type:

Article

Construction of a T7 phage random peptide library by combining seamless cloning with in vitro translation.

Published in:

Journal of Biochemistry, 2024, v. 175, n. 1, p. 85, doi. 10.1093/jb/mvad077

By:

Higashi, Katsuaki;
Oda, Sakiho;
Fujii, Mai;
Nishida, Fumiya;
Matsumoto, Hayato;
Morise, Jyoji;
Oka, Shogo;
Nonaka, Motohiro

Publication type:

Article

HUMAN REPRODUCTIVE CLONING, EMBRYO STEM CELLS AND GERMLINE GENE INTERVENTION: AN ISRAELI PERSPECTIVE.

Published in:

Medicine & Law (World Association for Medical Law), 2003, v. 22, n. 4, p. 701

By:

Revel, Michel

Publication type:

Article

One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants.

Published in:

New Phytologist, 2010, v. 187, n. 1, p. 240, doi. 10.1111/j.1469-8137.2010.03253.x

By:

Guoyong Xu;
Ning Sui;
Yang Tang;
Ke Xie;
Yizhen Lai;
Yule Liu

Publication type:

Article

Recombinant expression library of Pyrococcus furiosus constructed by high-throughput cloning: a useful tool for functional and structural genomics.

Published in:

Frontiers in Microbiology, 2015, p. 1, doi. 10.3389/fmicb.2015.00943

By:

Hui Yuan;
Li Peng;
Zhong Han;
Juan-Juan Xie;
Xi-Peng Liu

Publication type:

Article

NONLOCAL CLONING FOR A STATE NEAR A GIVEN ONE WITH SINGLE-PHOTON INTERFERENCE.

Published in:

International Journal of Quantum Information, 2009, v. 7, n. 7, p. 1331, doi. 10.1142/S0219749909005663

By:

YANG SHAO;
YAN-HUI ZHOU;
AI-DONG ZHU

Publication type:

Article

Cloning Adult Farm Animals: A Review of the Possibilities and Problems Associated with Somatic Cell Nuclear Transfer.

Published in:

American Journal of Reproductive Immunology, 2003, v. 50, n. 2, p. 113, doi. 10.1034/j.1600-0897.2003.00064.x

By:

Edwards, J. L.;
Schrick, F. N.;
McCracken, M. D.;
van Amstel, S. R.;
Hopkins, F. M.;
Welborn, M. G.;
Davies, C. J.

Publication type:

Article

Cloning and Sequencing of cDNA and Genomic DNA Encoding PDM Phosphatase of Fusarium moniliforme.

Published in:

Journal of Biochemistry, 2006, v. 140, n. 6, p. 813, doi. 10.1093/jb/mvj215

By:

Yoshida, Hiroshi;
Iizuka, Mari;
Narita, Takao;
Norioka, Naoko;
Norioka, Shigemi

Publication type:

Article

Animal cloning applications and issues.

Published in:

Russian Journal of Genetics, 2017, v. 53, n. 9, p. 965, doi. 10.1134/S102279541709006X

By:

Ibtisham, F.;
Fahd Qadir, M.;
Xiao, M.;
An, L.

Publication type:

Article

Human embryos cloned for stem cells: work not seen as leap toward reproductive cloning.

Published in:

2004

By:

Vastag, Brian

Publication type:

journal article

UN Delays Cloning Decision.

Published in:

JAMA: Journal of the American Medical Association, 2003, v. 290, n. 23, p. 3057, doi. 10.1001/jama.290.23.3057-d

By:

Stephenson, Joan

Publication type:

Article

A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments.

Published in:

BMC Research Notes, 2012, v. 5, n. 1, p. 1, doi. 10.1186/1756-0500-5-303

By:

Orlova, Nadezhda A.;
Orlov, Alexandre V.;
Vorobiev, Ivan I.

Publication type:

Article

Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes.

Published in:

Plant Methods, 2018, v. 14, n. 1, p. N.PAG, doi. 10.1186/s13007-018-0359-7

By:

Fischer, Matthew D.;
Mgboji, Emmanuel;
Liu, Zhongchi

Publication type:

Article

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system.

Published in:

Virology Journal, 2020, v. 17, n. 1, p. 1, doi. 10.1186/s12985-020-01358-2

By:

Bhat, Sushant;
Bialy, Dagmara;
Sealy, Joshua E.;
Sadeyen, Jean-Remy;
Chang, Pengxiang;
Iqbal, Munir

Publication type:

Article

Hybrid Quantum Cloning Machine.

Published in:

Quantum Information Processing, 2007, v. 6, n. 4, p. 197, doi. 10.1007/s11128-007-0053-6

By:

Adhikari, S.;
Pati, A.;
Chakrabarty, I.;
Choudhury, B.

Publication type:

Article

Chancen und Risiken der Reproduktionsbiotechnologien in der Tierzucht - Verfahren des Gene Editing und des Klonens.

Published in:

Züchtungskunde, 2024, v. 96, n. 1, p. 10

By:

HÖLKER, MICHAEL

Publication type:

Article

MCT cloning: a seamless cloning strategy for inserting DNA fragments.

Published in:

Biotechnology & Biotechnological Equipment, 2018, v. 32, n. 5, p. 1298, doi. 10.1080/13102818.2018.1507756

By:

Li, Wen-Ying;
Liu, Chang-Jun;
Wu, Lei;
Wu, Jin-Feng;
Yin, Xi-Nong;
Deng, Kai-Hang;
Zhang, Dong-Yi;
Meng, Er

Publication type:

Article