Works by Wittwer, Carl T

Results: 43

Advancing Laboratory Medicine through Innovation: A Tale of Six Inventors.

Published in:

Clinical Chemistry, 2012, v. 58, n. 3, p. 502, doi. 10.1373/clinchem.2011.178582

By:

Rifai, Nader;
Diamandis, Eleftherios P.;
Lo, Y. M. Dennis;
Kricka, Larry J.;
Wilding, Peter;
Ladenson, Jack H.;
Wittwer, Carl T.

Publication type:

Article

Snapback Primer Genotyping of the Gilbert Syndrome UGT1A1 (TA)<sub>n</sub> Promoter Polymorphism by High-Resolution Melting.

Published in:

Clinical Chemistry, 2011, v. 57, n. 9, p. 1303, doi. 10.1373/clinchem.2011.166306

By:

Farrar, Jared S.;
Palais, Robert A.;
Wittwer, Carl T.

Publication type:

Article

Primer Sequence Disclosure: A Clarification of the MIQE Guidelines.

Published in:

Clinical Chemistry, 2011, v. 57, n. 6, p. 919, doi. 10.1373/clinchem.2011.162958

By:

Bustin, Stephen A.;
Benes, Vladimir;
Garson, Jeremy A.;
Hellemans, Jan;
Huggett, Jim;
Kubista, Mikael;
Mueller, Reinhold;
Nolan, Tania;
Pfaffl, Michael W.;
Shipley, Gregory L.;
Vandesompele, Jo;
Wittwer, Carl T.

Publication type:

Article

Detecting Rare Thrombophilia Variants by High-Resolution Melting.

Published in:

Clinical Chemistry, 2011, v. 57, n. 4, p. 639, doi. 10.1373/clinchem.2010.159160

By:

Seipp, Michael T.;
Wittwer, Carl T.

Publication type:

Article

Making DNA Melting Useful.

Published in:

Clinical Chemistry, 2010, v. 56, n. 9, p. 1500, doi. 10.1373/clinchem.2010.146175

By:

Wittwer, Carl T.

Publication type:

Article

Enrichment and Detection of Rare Alleles by Means of Snapback Primers and Rapid-Cycle PCR.

Published in:

Clinical Chemistry, 2010, v. 56, n. 5, p. 814, doi. 10.1373/clinchem.2009.142034

By:

Zhou, Luming;
Palais, Robert A.;
Smith, G. Denice;
Anderson, Daniel;
Rowe, Leslie R.;
Wittwer, Carl T.

Publication type:

Article

Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons.

Published in:

Nucleic Acids Research, 2008, v. 36, n. 10, p. 3401, doi. 10.1093/nar/gkn204

By:

Gundry, Cameron N.;
Dobrowolski, Steven F.;
Martin, Y. Ranae;
Robbins, Thomas C.;
Nay, Lyle M.;
Boyd, Nathan;
Coyne, Thomas;
Wall, Mikeal D.;
Wittwer, Carl T.;
Teng, David H.-F.

Publication type:

Article

Automated DNA Extraction, Quantification, Dilution, and PCR Preparation for Genotyping by High-Resolution Melting.

Published in:

Journal of Biomolecular Techniques, 2010, v. 21, n. 4, p. 163

By:

Seipp, Michael T.;
Herrmann, Mark;
Wittwer, Carl T.

Publication type:

Article

Monovalent and divalent salt correction algorithms for Tm prediction—recommendations for Primer3 usage.

Published in:

Briefings in Bioinformatics, 2011, v. 12, n. 5, p. 514, doi. 10.1093/bib/bbq081

By:

von Ahsen, Nicolas;
Wittwer, Carl T.;
Schütz, Ekkehard

Publication type:

Article

Evaluation of Mass Cytometry in the Clinical Laboratory.

Published in:

Cytometry. Part B, 2019, v. 96, n. 4, p. 266, doi. 10.1002/cyto.b.21791

By:

Ravkov, Eugene V.;
Charlton, Cheryl M.;
Barker, Adam P.;
Hill, Harry;
Peterson, Lisa K.;
Slev, Patricia;
Tebo, Anne;
Voelkerding, Karl V.;
Wittwer, Carl T.;
Heikal, Nahla;
Delgado, Julio C.;
Lázár‐Molnár, Eszter;
Kumánovics, Attila

Publication type:

Article

Rare event counting of CD59− red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab.

Published in:

Cytometry. Part B, 2015, v. 88, n. 4, p. 261, doi. 10.1002/cyto.b.21225

By:

Liew, Michael;
Farley, Marjorie;
Andreasen, John;
Parker, Charles J.;
Wittwer, Carl T.

Publication type:

Article

Allele-Specific PCR with Competitive Probe Blocking for Sensitive and Specific Detection of BRAF V600E in Thyroid Fine-Needle Aspiration Specimens.

Published in:

Acta Cytologica, 2011, v. 55, n. 6, p. 576, doi. 10.1159/000333453

By:

Smith, G. Denice;
Zhou, Luming;
Rowe, Leslie R.;
Jarboe, Elke A.;
Collins, Brian T.;
Bentz, Joel S.;
Wittwer, Carl T.;
Chadwick, Barbara E.

Publication type:

Article

uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application.

Published in:

Bioinformatics, 2011, v. 27, n. 7, p. 1019, doi. 10.1093/bioinformatics/btr065

By:

Dwight, Zachary;
Palais, Robert;
Wittwer, Carl T.

Publication type:

Article

Clinical, ultrastructural immunohistochemical and DNA content analysis of lymphomas having features of interdigitating reticulum cells.

Published in:

1988

By:

Rabkin, Michael S.;
Kjeldsberg, Carl R.;
Hammond, M. Elizabeth;
Wittwer, Carl T.;
Nathwani, Bharat;
Rabkin, M S;
Kjeldsberg, C R;
Hammond, M E;
Wittwer, C T;
Nathwani, B

Publication type:

journal article

Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis.

Published in:

Nature Medicine, 2001, v. 7, n. 2, p. 249, doi. 10.1038/84708

By:

Elenitoba-Johnson, Kojo S.J.;
Bohling, Sandra D.;
Wittwer, Carl T.;
King, Thomas C.

Publication type:

Article

High-resolution DNA melting analysis: advancements and limitations.

Published in:

Human Mutation, 2009, v. 30, n. 6, p. 857, doi. 10.1002/humu.20951

By:

Wittwer, Carl T.

Publication type:

Article

Multiple endocrine neoplasia type 2 RET protooncogene database: Repository of MEN2-associated RET sequence variation and reference for genotype/phenotype correlations.

Published in:

2009

By:

Margraf, Rebecca L.;
Crockett, David K.;
Krautscheid, Patti M.F.;
Seamons, Ryan;
Calderon, Fernanda R.O.;
Wittwer, Carl T.;
Mao, Rong

Publication type:

Other

Masking selected sequence variation by incorporating mismatches into melting analysis probes.

Published in:

Human Mutation, 2006, v. 27, n. 3, p. 269, doi. 10.1002/humu.20290

By:

Margraf, Rebecca L.;
Mao, Rong;
Wittwer, Carl T.

Publication type:

Article

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines Free.

Published in:

Clinical Chemistry, 2025, v. 71, n. 6, p. 634, doi. 10.1093/clinchem/hvaf043

By:

Bustin, Stephen A;
Ruijter, Jan M;
Hoff, Maurice J B van den;
Kubista, Mikael;
Pfaffl, Michael W;
Shipley, Gregory L;
Tran, Nham;
Rödiger, Stefan;
Untergasser, Andreas;
Mueller, Reinhold;
Nolan, Tania;
Milavec, Mojca;
Burns, Malcolm J;
Huggett, Jim F;
Vandesompele, Jo;
Wittwer, Carl T

Publication type:

Article

Molecular Diagnostics: Going from Strength to Strength.

Published in:

Clinical Chemistry, 2020, v. 66, n. 1, p. 1, doi. 10.1093/clinchem.2019.314385

By:

Dennis Lo, Y. M.;
Baudhuin, Linnea M.;
Pantel, Klaus;
Chiu, Rossa W. K.;
Nolte, Frederick S.;
Wittwer, Carl T.

Publication type:

Article

Integrated Extreme Real-Time PCR and High-Speed Melting Analysis in 52 to 87 Seconds.

Published in:

Clinical Chemistry, 2019, v. 65, n. 2, p. 263, doi. 10.1373/clinchem.2018.296608

By:

Myrick, Joseph T.;
Pryor, Robert J.;
Palais, Robert A.;
Ison, Sean J.;
Sanford, Lindsay;
Dwight, Zachary L.;
Huuskonen, Jarkko J.;
Sundberg, Scott O.;
Wittwer, Carl T.

Publication type:

Article

A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.

Published in:

Clinical Chemistry, 2018, v. 64, n. 10, p. 1453, doi. 10.1373/clinchem.2018.290189

By:

Andini, Nadya;
Hu, Anne;
Luming Zhou;
Cogill, Steven;
Tza-Huei Wang;
Wittwer, Carl T.;
Yang, Samuel

Publication type:

Article

The Effect of Single Mismatches on Primer Extension.

Published in:

Clinical Chemistry, 2018, v. 64, n. 5, p. 801, doi. 10.1373/clinchem.2017.282285

By:

Rejali, Nick A.;
Moric, Endi;
Wittwer, Carl T.

Publication type:

Article

High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.

Published in:

Clinical Chemistry, 2017, v. 63, n. 10, p. 1624, doi. 10.1373/clinchem.2017.276147

By:

Pryor, Robert J.;
Myrick, Joseph T.;
Palais, Robert A.;
Sundberg, Scott O.;
Paek, Jeanette Y.;
Wittwer, Carl T.;
Knight, Ivor T.

Publication type:

Article

MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.

Published in:

Clinical Chemistry, 2017, v. 63, n. 9, p. 1537, doi. 10.1373/clinchem.2016.268953

By:

Bustin, Stephen A.;
Wittwer, Carl T.

Publication type:

Article

Democratizing the Real-Time PCR.

Published in:

Clinical Chemistry, 2017, v. 63, n. 4, p. 924, doi. 10.1373/clinchem.2016.263269

By:

Wittwer, Carl T.

Publication type:

Article

Portable Nanopore Sequencing for Viral Surveillance.

Published in:

Clinical Chemistry, 2016, v. 62, n. 11, p. 1427, doi. 10.1373/clinchem.2016.256693

By:

Wittwer, Carl T.

Publication type:

Article

Copy Number Assessment by Competitive PCR with Limiting Deoxynucleotide Triphosphates and High-Resolution Melting.

Published in:

Clinical Chemistry, 2015, v. 61, n. 5, p. 724, doi. 10.1373/clinchem.2014.236208

By:

Luming Zhou;
Palais, Robert A.;
Paxton, Christian N.;
Geiersbach, Katherine B.;
Wittwer, Carl T.

Publication type:

Article

Extreme PCR: Efficient and Specific DNA Amplification in 15-60 Seconds.

Published in:

Clinical Chemistry, 2015, v. 61, n. 1, p. 145, doi. 10.1373/clinchem.2014.228304

By:

Farrar, Jared S.;
Wittwer, Carl T.

Publication type:

Article

Molecular Diagnostics: A Revolution in Progress.

Published in:

Clinical Chemistry, 2015, v. 61, n. 1, p. 1, doi. 10.1373/clinchem.2014.233361

By:

Chiu, Rossa W. K.;
Lo, Y. M. Dennis;
Wittwer, Carl T.

Publication type:

Article

Microfluidic Genotyping by Rapid Serial PCR and High-Speed Melting Analysis.

Published in:

Clinical Chemistry, 2014, v. 60, n. 10, p. 1306, doi. 10.1373/clinchem.2014.223768

By:

Sundberg, Scott O.;
Wittwer, Carl T.;
Howell, Renée M.;
Huuskonen, Jarkko;
Pryor, Robert J.;
Farrar, Jared S.;
Stiles, Heather M.;
Palais, Robert A.;
Knight, Ivor T.

Publication type:

Article

Genotyping Accuracy of High-Resolution DNA Melting Instruments.

Published in:

Clinical Chemistry, 2014, v. 60, n. 6, p. 864, doi. 10.1373/clinchem.2013.220160

By:

Mei Li;
Luming Zhou;
Palais, Robert A.;
Wittwer, Carl T.

Publication type:

Article

Influence of PCR Reagents on DNA Polymerase Extension Rates Measured on Real-Time PCR Instruments.

Published in:

Clinical Chemistry, 2014, v. 60, n. 2, p. 334, doi. 10.1373/clinchem.2013.212829

By:

Montgomery, Jesse L.;
Wittwer, Carl T.

Publication type:

Article

Symmetric Snapback Primers for Scanning and Genotyping of the Cystic Fibrosis Transmembrane Conductance Regulator Gene.

Published in:

Clinical Chemistry, 2013, v. 59, n. 7, p. 1052, doi. 10.1373/clinchem.2013.202689

By:

Luming Zhou;
Palais, Robert A.;
Ye, Felix;
Chen, Jimmy;
Montgomery, Jesse L.;
Wittwer, Carl T.

Publication type:

Article

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Published in:

Clinical Chemistry, 2013, v. 59, n. 6, p. 892, doi. 10.1373/clinchem.2013.206375

By:

Huggett, Jim F.;
Foy, Carole A.;
Benes, Vladimir;
Emslie, Kerry;
Garson, Jeremy A.;
Haynes, Ross;
Hellemans, Jan;
Kubista, Mikael;
Mueller, Reinhold D.;
Nolan, Tania;
Pfaffl, Michael W.;
Shipley, Gregory L.;
Vandesompele, Jo;
Wittwer, Carl T.;
Bustin, Stephen A.

Publication type:

Article

Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S) in combination with EPAS1 (HIF-2α) polymorphism lowers hemoglobin concentration in Tibetan highlanders.

Published in:

Journal of Molecular Medicine, 2017, v. 95, n. 6, p. 665, doi. 10.1007/s00109-017-1519-3

By:

Tashi, Tsewang;
Scott Reading, N.;
Wuren, Tanna;
Zhang, Xu;
Moore, Lorna;
Hu, Hao;
Tang, Feng;
Shestakova, Anna;
Lorenzo, Felipe;
Burjanivova, Tatiana;
Koul, Parvaiz;
Guchhait, Prasenjit;
Wittwer, Carl T.;
Julian, Colleen;
Shah, Binal;
Huff, Chad;
Gordeuk, Victor;
Prchal, Josef;
Ge, RiLi

Publication type:

Article

Nearest-neighbour transition-state analysis for nucleic acid kinetics.

Published in:

Nucleic Acids Research, 2021, v. 49, n. 8, p. 4574, doi. 10.1093/nar/gkab205

By:

Rejali, Nick A.;
Ye, Felix D.;
Zuiter, Aisha M.;
Keller, Caroline C.;
Wittwer, Carl T.

Publication type:

Article

Removal of artifact bias from qPCR results using DNA melting curve analysis.

Published in:

FASEB Journal, 2019, v. 33, n. 12, p. 14542, doi. 10.1096/fj.201901604R

By:

Ruijter, Jan M.;
Ruiz-Villalba, Adrian;
van den Hoff, Axel J. J.;
Gunst, Quinn D.;
Wittwer, Carl T.;
van den Hoff, Maurice J. B.

Publication type:

Article

The need for transparency and good practices in the qPCR literature.

Published in:

Nature Methods, 2013, v. 10, n. 11, p. 1063, doi. 10.1038/nmeth.2697

By:

Bustin, Stephen A;
Benes, Vladimir;
Garson, Jeremy;
Hellemans, Jan;
Huggett, Jim;
Kubista, Mikael;
Mueller, Reinhold;
Nolan, Tania;
Pfaffl, Michael W;
Shipley, Gregory;
Wittwer, Carl T;
Schjerling, Peter;
Day, Philip J;
Abreu, Mónica;
Aguado, Begoña;
Beaulieu, Jean-François;
Beckers, Anneleen;
Bogaert, Sara;
Browne, John A;
Carrasco-Ramiro, Fernando

Publication type:

Article

Genomic Copy Number Analysis of HER2-Equivocal Breast Cancers.

Published in:

2016

By:

Geiersbach, Katherine B;
Willmore-Payne, Carlynn;
Pasi, Alexandra V;
Paxton, Christian N;
Werner, Theresa L;
Xu, Xinjie;
Wittwer, Carl T;
Gulbahce, H Evin;
Downs-Kelly, Erinn

Publication type:

journal article

Heterozygote PCR Product Melting Curve Prediction.

Published in:

Human Mutation, 2014, v. 35, n. 3, p. 278, doi. 10.1002/humu.22494

By:

Dwight, Zachary L.;
Palais, Robert;
Kent, Jana;
Wittwer, Carl T.

Publication type:

Article

Molecular classification of melanoma using real‐time quantitative reverse transcriptase‐polymerase chain reaction.

Published in:

Cancer (0008543X), 2005, v. 104, n. 8, p. 1678

By:

Tracey B. Lewis;
John E. Robison;
Roy Bastien;
Brett Milash;
Ken Boucher;
Wolfram E. Samlowski;
Sancy A. Leachman;
R. Dirk Noyes;
Carl T. Wittwer;
Laurent Perreard;
Philip S. Bernard

Publication type:

Article

Evaluation of HER-2/neu Gene Status in Osteosarcoma by Fluorescence In Situ Hybridization and Multiplex and Monoplex Polymerase Chain Reactions.

Published in:

Archives of Pathology & Laboratory Medicine, 2006, v. 130, n. 5, p. 691, doi. 10.5858/2006-130-691-eongsi

By:

Willmore-Payne, Carlynn;
Holden, Joseph A.;
Zhou, Holly;
Gupta, Dilip;
Hirschowitz, Sharon;
Wittwer, Carl T.;
Layfield, Lester J.

Publication type:

Article