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Construction of hybrid gene libraries involving the circular permutation of DNA.
- Published in:
- Biotechnology Letters, 2001, v. 23, n. 4, p. 303, doi. 10.1023/A:1005642725295
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- Article
Characterization of Monobody Scaffold Interactions with Ligand via Force Spectroscopy and Steered Molecular Dynamics.
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- Scientific Reports, 2015, p. 8247, doi. 10.1038/srep08247
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- Article
Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria.
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- PLoS ONE, 2018, v. 13, n. 12, p. 1, doi. 10.1371/journal.pone.0209408
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- Article
Solvent-Dependent Oxidation of a (Pyridylmethyl)amino Ligand by FeCl<sub>3</sub> To Give a Water-Soluble Blue Fluorophore.
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- Angewandte Chemie International Edition, 2007, v. 46, n. 28, p. 5329, doi. 10.1002/anie.200700389
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- Article
Comprehensive mutagenesis on yeast cytosine deaminase yields improvements in 5‐fluorocytosine toxicity in HT1080 cells.
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- AIChE Journal, 2020, v. 66, n. 3, p. N.PAG, doi. 10.1002/aic.16688
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- Article
Development of a cancer-marker activated enzymatic switch from the herpes simplex virus thymidine kinase.
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- PEDS: Protein Engineering, Design & Selection, 2017, v. 30, n. 2, p. 95, doi. 10.1093/protein/gzw067
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- Article
Enzyme stabilization by domain insertion into a thermophilic protein.
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- PEDS: Protein Engineering, Design & Selection, 2009, v. 22, n. 10, p. 615, doi. 10.1093/protein/gzp044
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- Article
Engineering allosteric protein switches by domain insertion.
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- PEDS: Protein Engineering, Design & Selection, 2005, v. 18, n. 8, p. 359, doi. 10.1093/protein/gzi048
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- Article
The High-Affinity Maltose Switch MBP317-347 has Low Affinity for Glucose: Implications for Targeting Tumors with Metabolically Directed Enzyme Prodrug Therapy.
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- Chemical Biology & Drug Design, 2014, v. 83, n. 3, p. 266, doi. 10.1111/cbdd.12249
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- Article
Fitness and Functional Landscapes of the E. coli RNase III Gene rnc.
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- Molecular Biology & Evolution, 2023, v. 40, n. 3, p. 1, doi. 10.1093/molbev/msad047
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- Article
Genes Vary Greatly in Their Propensity for Collateral Fitness Effects of Mutations.
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- Molecular Biology & Evolution, 2023, v. 40, n. 3, p. 1, doi. 10.1093/molbev/msad038
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- Article
A Comprehensive, High-Resolution Map of a Gene’s Fitness Landscape.
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- Molecular Biology & Evolution, 2014, v. 31, n. 6, p. 1581, doi. 10.1093/molbev/msu081
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- Article
Complexes of Click-Derived Bistriazolylpyridines: Remarkable Electronic Influence of Remote Substituents on Thermodynamic Stability as well as Electronic and Magnetic Properties.
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- Chemistry - A European Journal, 2010, v. 16, n. 33, p. 10202, doi. 10.1002/chem.201000721
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- Article
Enzymatic protein switches built from paralogous input domains.
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- Biotechnology & Bioengineering, 2016, v. 113, n. 4, p. 852, doi. 10.1002/bit.25852
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- Article
Electrochemical Activation of Engineered Protein Switches.
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- Biotechnology & Bioengineering, 2016, v. 113, n. 2, p. 453, doi. 10.1002/bit.25720
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- Article
Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA.
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- Biotechnology & Bioengineering, 2011, v. 108, n. 11, p. 2535, doi. 10.1002/bit.23224
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- Article
Design of protein switches based on an ensemble model of allostery.
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- Nature Communications, 2015, v. 6, n. 4, p. 6968, doi. 10.1038/ncomms7968
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- Article
A bacterial dual positive and negative selection system for dCas9 activity.
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- PLoS ONE, 2022, v. 17, n. 6, p. 1, doi. 10.1371/journal.pone.0269270
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- Article
Engineered protein switches for exogenous control of gene expression.
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- Biochemical Society Transactions, 2020, v. 48, n. 5, p. 2205, doi. 10.1042/BST20200441
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- Article
The Coordination Chemistry of Iron with the 1,4-Bis(2-pyridyl-methyl)piperazine Ligand.
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- Zeitschrift für Anorganische und Allgemeine Chemie, 2006, v. 632, n. 7, p. 1287, doi. 10.1002/zaac.200600003
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- Article
The interplay between effector binding and allostery in an engineered protein switch.
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- Protein Science: A Publication of the Protein Society, 2016, v. 25, n. 9, p. 1605, doi. 10.1002/pro.2962
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- Article
Non-allosteric enzyme switches possess larger effector-induced changes in thermodynamic stability than their non-switch analogs.
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- Protein Science: A Publication of the Protein Society, 2013, v. 22, n. 4, p. 475, doi. 10.1002/pro.2234
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- Article
Ligand binding and allostery can emerge simultaneously.
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- Protein Science: A Publication of the Protein Society, 2007, v. 16, n. 5, p. 929, doi. 10.1110/ps.062706007
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- Article
A combinatorial approach to hybrid enzymes independent of DNA homology.
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- Nature Biotechnology, 1999, v. 17, n. 12, p. 1205, doi. 10.1038/70754
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- Article
Finding Cinderella's slipper?proteins that fit.
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- Nature Biotechnology, 1999, v. 17, n. 7, p. 639, doi. 10.1038/10850
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- Article
Advancements in the Development of HIF-1α-Activated Protein Switches for Use in Enzyme Prodrug Therapy.
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- PLoS ONE, 2014, v. 9, n. 11, p. 1, doi. 10.1371/journal.pone.0114032
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- Article
Directed Evolution of Improved Zinc Finger Methyltransferases.
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- PLoS ONE, 2014, v. 9, n. 5, p. 1, doi. 10.1371/journal.pone.0096931
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- Article
PFunkel: Efficient, Expansive, User-Defined Mutagenesis.
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- PLoS ONE, 2012, v. 7, n. 12, p. 1, doi. 10.1371/journal.pone.0052031
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- Article
Targeted DNA Methylation Using an Artificially Bisected M.HhaI Fused to Zinc Fingers.
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- PLoS ONE, 2012, v. 7, n. 9, p. 1, doi. 10.1371/journal.pone.0044852
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- Article
Structure of an Engineered b-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation.
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- PLoS ONE, 2012, v. 7, n. 6, p. 1, doi. 10.1371/journal.pone.0039168
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- Article
Circular Permutation in the Ω-Loop of TEM-1 β-Lactamase Results in Improved Activity and Altered Substrate Specificity.
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- PLoS ONE, 2012, v. 7, n. 4, p. 1, doi. 10.1371/journal.pone.0035998
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- Article
In Vitro Recombination of Non-Homologous Genes Can Result in Gene Fusions that Confer a Switching Phenotype to Cells.
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- PLoS ONE, 2011, v. 6, n. 11, p. 1, doi. 10.1371/journal.pone.0027302
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- Article
Multifunctional 'Clickates' as Versatile Extended Heteroaromatic Building Blocks: Efficient Synthesis via Click Chemistry, Conformational Preferences, and Metal Coordination.
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- Chemistry - A European Journal, 2007, v. 13, n. 35, p. 9834, doi. 10.1002/chem.200701240
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- Article
NMR characterization of an engineered domain fusion between maltose binding protein and TEM1 β-lactamase provides insight into its structure and allosteric mechanism.
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- Proteins, 2010, v. 78, n. 6, p. 1423, doi. 10.1002/prot.22657
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- Article
Theoretical distribution of truncation lengths in incremental truncation libraries.
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- Biotechnology & Bioengineering, 2003, v. 82, n. 5, p. 564, doi. 10.1002/bit.10604
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- Article
Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression.
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- Nucleic Acids Research, 2016, v. 44, n. 21, p. 10515, doi. 10.1093/nar/gkw981
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- Article
The genetic code constrains yet facilitates Darwinian evolution.
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- Nucleic Acids Research, 2013, v. 41, n. 15, p. 7420, doi. 10.1093/nar/gkt536
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- Article
A Positive Selection for Nucleoside Kinases in E. coli.
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- PLoS ONE, 2016, v. 11, n. 9, p. 1, doi. 10.1371/journal.pone.0162921
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- Article
Heterodimeric DNA methyltransferases as a platform for creating designer zinc finger methyltransferases for targeted DNA methylation in cells.
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- Nucleic Acids Research, 2010, v. 38, n. 5, p. 1749, doi. 10.1093/nar/gkp1126
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- Article
Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides.
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- Nucleic Acids Research, 2001, v. 29, n. 4, p. e16, doi. 10.1093/nar/29.4.e16
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- Article
A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination.
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- Biotechnology for Biofuels, 2016, v. 9, p. 1, doi. 10.1186/s13068-016-0529-7
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- Article
Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase.
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- Biotechnology for Biofuels, 2015, v. 8, n. 1, p. 1, doi. 10.1186/s13068-015-0293-0
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- Article