Works by Nagai, Takeharu

Results: 82

Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control.

Published in:

PLoS Biology, 2004, v. 2, n. 6, p. 1, doi. 10.1371/journal.pbio.0020163

By:

Hasan, Mazahir T;
Friedrich, Rainer W;
Euler, Thomas;
Larkum, Matthew E;
Giese, Günter;
Both, Matthias;
Duebel, Jens;
Waters, Jack;
Bujard, Hermann;
Griesbeck, Oliver;
Tsien, Roger Y;
Nagai, Takeharu;
Miyawaki, Atsushi;
Denk, Winfried

Publication type:

Article

Functional Fluorescent Ca... Indicator Proteins in Transgenic Mice under TET Control.

Published in:

PLoS Biology, 2004, v. 2, n. 6, p. 763, doi. 10.1371/journal.pbio.0020163

By:

Hasan, Mazahir T.;
Friedrich, Rainer W.;
Euler, Thomas;
Larkum, Matthew E.;
Giese, Günter;
Both, Matthias;
Duebel, Jens;
Waters, Jack;
Bujard, Hermann;
Griesbeck, Oliver;
Tsien, Roger Y.;
Nagai, Takeharu;
Miyawaki, Atsushi;
Denk, Winfried

Publication type:

Article

Significance of PGR5-dependent cyclic electron flow for optimizing the rate of ATP synthesis and consumption in Arabidopsis chloroplasts.

Published in:

Photosynthesis Research, 2019, v. 139, n. 1-3, p. 359, doi. 10.1007/s11120-018-0533-9

By:

Sato, Ryoichi;
Kawashima, Rinya;
Trinh, Mai Duy Luu;
Nakano, Masahiro;
Nagai, Takeharu;
Masuda, Shinji

Publication type:

Article

Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue.

Published in:

Development Genes & Evolution, 2001, v. 211, n. 6, p. 309, doi. 10.1007/s004270100157

By:

Kitaguchi, Tetsuya;
Nakata, Katsunori;
Nagai, Takeharu;
Aruga, Jun;
Mikoshiba, Katsuhiko

Publication type:

Article

SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.

Published in:

Scientific Reports, 2013, p. 1, doi. 10.1038/srep02629

By:

Kiwamu Takemoto;
Tomoki Matsuda;
Naoki Sakai;
Donald Fu;
Masanori Noda;
Susumu Uchiyama;
Ippei Kotera;
Yoshiyuki Arai;
Masataka Horiuchi;
Kiichi Fukui;
Tokiyoshi Ayabe;
Fuyuhiko Inagaki;
Hiroshi Suzuki;
Takeharu Nagai

Publication type:

Article

Publisher Correction: High-throughput fluorescence lifetime imaging flow cytometry.

Published in:

2025

By:

Kanno, Hiroshi;
Hiramatsu, Kotaro;
Mikami, Hideharu;
Nakayashiki, Atsushi;
Yamashita, Shota;
Nagai, Arata;
Okabe, Kohki;
Li, Fan;
Yin, Fei;
Tominaga, Keita;
Bicer, Omer Faruk;
Noma, Ryohei;
Kiani, Bahareh;
Efa, Olga;
Büscher, Martin;
Wazawa, Tetsuichi;
Sonoshita, Masahiro;
Shintaku, Hirofumi;
Nagai, Takeharu;
Braun, Sigurd

Publication type:

Correction Notice

High-throughput fluorescence lifetime imaging flow cytometry.

Published in:

Nature Communications, 2024, v. 15, n. 1, p. 1, doi. 10.1038/s41467-024-51125-y

By:

Kanno, Hiroshi;
Hiramatsu, Kotaro;
Mikami, Hideharu;
Nakayashiki, Atsushi;
Yamashita, Shota;
Nagai, Arata;
Okabe, Kohki;
Li, Fan;
Yin, Fei;
Tominaga, Keita;
Bicer, Omer Faruk;
Noma, Ryohei;
Kiani, Bahareh;
Efa, Olga;
Büscher, Martin;
Wazawa, Tetsuichi;
Sonoshita, Masahiro;
Shintaku, Hirofumi;
Nagai, Takeharu;
Braun, Sigurd

Publication type:

Article

A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.

Published in:

Nature Biotechnology, 2002, v. 20, n. 1, p. 87, doi. 10.1038/nbt0102-87

By:

Nagai, Takeharu;
Ibata, Keiji;
Park, Eun Sun;
Kubota, Mie;
Mikoshiba, Katsuhiko;
Miyawaki, Atsushi

Publication type:

Article

Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.

Published in:

BMC Biology, 2018, v. 16, n. 1, p. 1, doi. 10.1186/s12915-018-0514-7

By:

Yemima Dani Riani;
Tomoki Matsuda;
Kiwamu Takemoto;
Takeharu Nagai

Publication type:

Article

Mesoscale heterogeneity is a critical determinant for spiral pattern formation in developing social amoeba.

Published in:

Scientific Reports, 2025, v. 15, n. 1, p. 1, doi. 10.1038/s41598-025-85759-9

By:

Kakizuka, Taishi;
Nakaoka, Hidenori;
Hara, Yusuke;
Ichiraku, Aya;
Arai, Yoshiyuki;
Itoga, Hiroya;
Onami, Shuichi;
Ichimura, Taro;
Nagai, Takeharu;
Horikawa, Kazuki

Publication type:

Article

Visualization of Synaptic Ca2+/Calmodulin-Dependent Protein Kinase II Activity in Living Neurons.

Published in:

Journal of Neuroscience, 2005, v. 25, n. 12, p. 3107, doi. 10.1523/JNEUROSCI.0085-05.2005

By:

Takao, Keizo;
Okamoto, Ken-Ichi;
Nakagawa, Terunaga;
Neve, Rachael L.;
Nagai, Takeharu;
Miyawaki, Atsushi;
Hashikawa, Tsutomu;
Kobayashi, Shigeo;
Hayashi, Yasunori

Publication type:

Article

Identification of Mitochondrial DNA Polymorphisms That Alter Mitochondrial Matrix pH and Intracellular Calcium Dynamics.

Published in:

PLoS Genetics, 2006, v. 3, n. 2, p. e128, doi. 10.1371/journal.pgen.0020128

By:

Kazuno, An-a;
Munakata, Kae;
Nagai, Takeharu;
Shimozono, Satoshi;
Tanaka, Masashi;
Yoneda, Makoto;
Kato, Nobumasa;
Miyawaki, Atsushi;
Kato, Tadafumi;
Orr, Harry

Publication type:

Article

Changes in Cytosolic ATP Levels and Intracellular Morphology during Bacteria-Induced Hypersensitive Cell Death as Revealed by Real-Time Fluorescence Microscopy Imaging.

Published in:

Plant & Cell Physiology, 2012, v. 53, n. 10, p. 1768, doi. 10.1093/pcp/pcs119

By:

Hatsugai, Noriyuki;
Perez Koldenkova, Vadim;
Imamura, Hiromi;
Noji, Hiroyuki;
Nagai, Takeharu

Publication type:

Article

Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response.

Published in:

PLoS ONE, 2017, v. 12, n. 2, p. 1, doi. 10.1371/journal.pone.0172344

By:

Nakano, Masahiro;
Arai, Yoshiyuki;
Kotera, Ippei;
Okabe, Kohki;
Kamei, Yasuhiro;
Nagai, Takeharu

Publication type:

Article

Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength.

Published in:

PLoS ONE, 2016, v. 11, n. 10, p. 1, doi. 10.1371/journal.pone.0165148

By:

Lu-Walther, Hui-Wen;
Hou, Wenya;
Kielhorn, Martin;
Arai, Yoshiyuki;
Nagai, Takeharu;
Kessels, Michael M.;
Qualmann, Britta;
Heintzmann, Rainer

Publication type:

Article

Fluorescent Protein-Based Indicators for Functional Super-Resolution Imaging of Biomolecular Activities in Living Cells.

Published in:

International Journal of Molecular Sciences, 2019, v. 20, n. 22, p. 5784, doi. 10.3390/ijms20225784

By:

Lu, Kai;
Vu, Cong Quang;
Matsuda, Tomoki;
Nagai, Takeharu

Publication type:

Article

Fluorescent Proteins for Investigating Biological Events in Acidic Environments.

Published in:

International Journal of Molecular Sciences, 2018, v. 19, n. 6, p. 1548, doi. 10.3390/ijms19061548

By:

Shinoda, Hajime;
Shannon, Michael;
Nagai, Takeharu

Publication type:

Article

Ultrasensitive Imaging of Ca2+ Dynamics in Pancreatic Acinar Cells of Yellow Cameleon-Nano Transgenic Mice.

Published in:

International Journal of Molecular Sciences, 2014, v. 15, n. 11, p. 19971, doi. 10.3390/ijms151119971

By:

Yusuke Oshima;
Takeshi Imamura;
Atsuko Shintani;
Hiroko Kajiura-Kobayashi;
Terumasa Hibi;
Takeharu Nagai;
Shigenori Nonaka;
Tomomi Nemoto

Publication type:

Article

Development of intensiometric indicators for visualizing N-cadherin interaction across cells.

Published in:

Communications Biology, 2022, v. 5, n. 1, p. 1, doi. 10.1038/s42003-022-04023-2

By:

Kanadome, Takashi;
Hayashi, Kanehiro;
Seto, Yusuke;
Eiraku, Mototsugu;
Nakajima, Kazunori;
Nagai, Takeharu;
Matsuda, Tomoki

Publication type:

Article

Spatio-temporal activation of caspase revealed by indicator that is sensitive to environmental effects.

Published in:

Journal of Cell Biology, 2003, v. 160, n. 2, p. 235, doi. 10.1083/jcb.200207111

By:

Takemoto, Kiwamu;
Nagai, Takeharu;
Miyawaki, Atsushi;
Miura, Masayuki

Publication type:

Article

Flexible and dynamic nucleosome fiber in living mammalian cells.

Published in:

Nucleus (1949-1034), 2013, v. 4, n. 5, p. 349, doi. 10.4161/nucl.26053

By:

Nozaki, Tadasu;
Kaizu, Kazunari;
Pack, Chan-Gi;
Tamura, Sachiko;
Tani, Tomomi;
Hihara, Saera;
Nagai, Takeharu;
Takahashi, Koichi;
Maeshima, Kazuhiro

Publication type:

Article

Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy.

Published in:

BMC Research Notes, 2018, v. 11, n. 1, p. N.PAG, doi. 10.1186/s13104-018-3421-7

By:

Suzuki, Kazushi;
Onishi, Takahito;
Nakada, Chieko;
Takei, Shunsuke;
Daniels, Matthew J.;
Nakano, Masahiro;
Matsuda, Tomoki;
Nagai, Takeharu

Publication type:

Article

An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease.

Published in:

PLoS ONE, 2018, v. 13, n. 4, p. 1, doi. 10.1371/journal.pone.0194707

By:

Hara-Kuge, Sayuri;
Nishihara, Tomonobu;
Matsuda, Tomoki;
Kitazono, Tomohiro;
Teramoto, Takayuki;
Nagai, Takeharu;
Ishihara, Takeshi

Publication type:

Article

Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics.

Published in:

PLoS ONE, 2017, v. 12, n. 4, p. 1, doi. 10.1371/journal.pone.0174181

By:

Chang, Yu-Fen;
Broyles, Connor N.;
Brook, Frances A.;
Davies, Mark J.;
Turtle, Cameron W.;
Daniels, Matthew J.;
Nagai, Takeharu

Publication type:

Article

Structure‐based analysis and evolution of a monomerized red‐colored chromoprotein from the Olindias formosa jellyfish.

Published in:

Protein Science: A Publication of the Protein Society, 2022, v. 31, n. 5, p. 1, doi. 10.1002/pro.4285

By:

Zhai, Le;
Nakashima, Ryosuke;
Shinoda, Hajime;
Ike, Yoshimasa;
Matsuda, Tomoki;
Nagai, Takeharu

Publication type:

Article

「今のめり込み術」のマルチ効能.

Published in:

Seibutsu Butsuri, 2014, v. 54, n. 2, p. 075, doi. 10.2142/biophys.54.075

By:

Nagai Takeharu

Publication type:

Article

Development of BRET based Ca2+ Indicator.

Published in:

Seibutsu Butsuri, 2012, v. 52, n. 1, p. 30, doi. 10.2142/biophys.52.030

By:

SAITO, Kenta;
NAGAI, Takeharu

Publication type:

Article

Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo.

Published in:

eLife, 2025, p. 1, doi. 10.7554/eLife.93633

By:

Taro Ichimura;
Taishi Kakizuka;
Yoshitsugu Taniguchi;
Satoshi Ejima;
Yuki Sato;
Keiko Itano;
Kaoru Seiriki;
Hitoshi Hashimoto;
Ko Sugawara;
Hiroya Itoga;
Shuichi Onami;
Takeharu Nagai

Publication type:

Article

A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging.

Published in:

Microscopy, 2021, v. 70, n. 4, p. 340, doi. 10.1093/jmicro/dfab001

By:

Wazawa, Tetsuichi;
Noma, Ryohei;
Uto, Shusaku;
Sugiura, Kazunori;
Washio, Takashi;
Nagai, Takeharu

Publication type:

Article

Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction.

Published in:

Microscopy, 2018, v. 67, n. 2, p. 89, doi. 10.1093/jmicro/dfy004

By:

Tetsuichi Wazawa;
Yoshiyuki Arai;
Yoshinobu Kawahara;
Hiroki Takauchi;
Takashi Washio;
Takeharu Nagai

Publication type:

Article

Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy.

Published in:

Microscopy, 2017, v. 66, n. 2, p. 110, doi. 10.1093/jmicro/dfw110

By:

Sang-Yeob Kim;
Yoshiyuki Arai;
Tomomi Tani;
Hirofumi Takatsuka;
Yoshiharu Saito;
Takayuki Kawashima;
Shojiro Kawakami;
Atsushi Miyawaki;
Takeharu Nagai

Publication type:

Article

A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy.

Published in:

Microscopy, 2015, v. 64, n. 4, p. 263, doi. 10.1093/jmicro/dfv037

By:

Shin-nosuke Uno;
Tiwari, Dhermendra K.;
Mako Kamiya;
Yoshiyuki Arai;
Takeharu Nagai;
Yasuteru Urano

Publication type:

Article

A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy.

Published in:

Microscopy, 2015, v. 64, n. 4, p. 263, doi. 10.1093/jmicro/dfv037

By:

Shin-nosuke Uno;
Tiwari, Dhermendra K.;
Mako Kamiya;
Yoshiyuki Arai;
Takeharu Nagai;
Yasuteru Urano

Publication type:

Article

Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins.

Published in:

Microscopy, 2014, v. 63, n. 6, p. 403, doi. 10.1093/jmicro/dfu033

By:

Tomoki Matsuda;
Takeharu Nagai

Publication type:

Article

Extensive use of FRET in biological imaging.

Published in:

Microscopy, 2013, v. 62, n. 4, p. 419, doi. 10.1093/jmicro/dft037

By:

Arai, Yoshiyuki;
Nagai, Takeharu

Publication type:

Article

Activity-dependent organization of prefrontal hub-networks for associative learning and signal transformation.

Published in:

Nature Communications, 2023, v. 14, n. 1, p. 1, doi. 10.1038/s41467-023-41547-5

By:

Agetsuma, Masakazu;
Sato, Issei;
Tanaka, Yasuhiro R.;
Carrillo-Reid, Luis;
Kasai, Atsushi;
Noritake, Atsushi;
Arai, Yoshiyuki;
Yoshitomo, Miki;
Inagaki, Takashi;
Yukawa, Hiroshi;
Hashimoto, Hitoshi;
Nabekura, Junichi;
Nagai, Takeharu

Publication type:

Article

Application of Green‐enhanced Nano‐lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH.

Published in:

Plant, Cell & Environment, 2022, v. 45, n. 10, p. 3157, doi. 10.1111/pce.14404

By:

Tran, Quang;
Osabe, Kenji;
Entani, Tetsuyuki;
Wazawa, Tetsuichi;
Hattori, Mitsuru;
Nagai, Takeharu

Publication type:

Article

Five colour variants of bright luminescent protein for real-time multicolour bioimaging.

Published in:

Nature Communications, 2016, v. 7, n. 12, p. 13718, doi. 10.1038/ncomms13718

By:

Suzuki, Kazushi;
Kimura, Taichi;
Shinoda, Hajime;
Bai, Guirong;
Daniels, Matthew J.;
Arai, Yoshiyuki;
Nakano, Masahiro;
Nagai, Takeharu

Publication type:

Article

Arl3 and LC8 regulate dissociation of dynactin from dynein.

Published in:

Nature Communications, 2014, v. 5, n. 10, p. 5295, doi. 10.1038/ncomms6295

By:

Jin, Mingyue;
Yamada, Masami;
Arai, Yoshiyuki;
Nagai, Takeharu;
Hirotsune, Shinji

Publication type:

Article

Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement.

Published in:

Nature Communications, 2013, v. 4, n. 6, p. 2033, doi. 10.1038/ncomms3033

By:

Yamada, Masami;
Kumamoto, Kanako;
Mikuni, Shintaro;
Arai, Yoshiyuki;
Kinjo, Masataka;
Nagai, Takeharu;
Tsukasaki, Yoshikazu;
Watanabe, Tomonobu M;
Fukui, Mitsuru;
Jin, Mingyue;
Toba, Shiori;
Hirotsune, Shinji

Publication type:

Article

Luminescent proteins for high-speed single-cell and whole-body imaging.

Published in:

Nature Communications, 2012, v. 3, n. 12, p. 1262, doi. 10.1038/ncomms2248

By:

Saito, Kenta;
Chang, Y-F;
Horikawa, Kazuki;
Hatsugai, Noriyuki;
Higuchi, Yuriko;
Hashida, Mitsuru;
Yoshida, Yu;
Matsuda, Tomoki;
Arai, Yoshiyuki;
Nagai, Takeharu

Publication type:

Article

MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.

Published in:

Plant Physiology, 2025, v. 197, n. 1, p. 1, doi. 10.1093/plphys/kiae613

By:

Iwano, Megumi;
Suetsugu, Noriyuki;
Nishihama, Ryuichi;
Ishida, Sakiko;
Horie, Tomoaki;
Costa, Alex;
Katsuno, Tatsuya;
Kimura, Midori;
Iida, Kazuko;
Iida, Hidetoshi;
Nagai, Takeharu;
Kohchi, Takayuki

Publication type:

Article

Optical inactivation of intracellular molecules by fast-maturating photosensitizing fluorescence protein, HyperNova.

Published in:

Communications Biology, 2024, v. 7, n. 1, p. 1, doi. 10.1038/s42003-024-06583-x

By:

Shidara, Hisashi;
Shirai, Taku;
Ozaki-Noma, Ryohei;
Jitsuki, Susumu;
Nagai, Takeharu;
Takemoto, Kiwamu

Publication type:

Article

High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant.

Published in:

Communications Biology, 2024, v. 7, n. 1, p. 1, doi. 10.1038/s42003-024-06394-0

By:

Hara, Yusuke;
Ichiraku, Aya;
Matsuda, Tomoki;
Sakane, Ayuko;
Sasaki, Takuya;
Nagai, Takeharu;
Horikawa, Kazuki

Publication type:

Article

Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cells.

Published in:

EMBO Journal, 2001, v. 20, n. 17, p. 4998, doi. 10.1093/emboj/20.17.4998

By:

Robert, Valerie;
Gurlini, Pamela;
Tosello, Valeria;
Nagai, Takeharu;
Miyawaki, Atsushi;
Di Lisa, Fabio;
Pozzan, Tullio

Publication type:

Article

A fast- and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy.

Published in:

Nature Methods, 2015, v. 12, n. 6, p. 515, doi. 10.1038/nmeth.3362

By:

Tiwari, Dhermendra K;
Arai, Yoshiyuki;
Yamanaka, Masahito;
Matsuda, Tomoki;
Agetsuma, Masakazu;
Nakano, Masahiro;
Fujita, Katsumasa;
Nagai, Takeharu

Publication type:

Article

Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano.

Published in:

Nature Methods, 2010, v. 7, n. 9, p. 729, doi. 10.1038/nmeth.1488

By:

Horikawa, Kazuki;
Yamada, Yoshiyuki;
Matsuda, Tomoki;
Kobayashi, Kentarou;
Hashimoto, Mitsuhiro;
Matsu-ura, Toru;
Miyawaki, Atsushi;
Michikawa, Takayuki;
Mikoshiba, Katsuhiko;
Nagai, Takeharu

Publication type:

Article

An ultramarine fluorescent protein with increased photostability and pH insensitivity.

Published in:

Nature Methods, 2009, v. 6, n. 5, p. 351, doi. 10.1038/nmeth.1317

By:

Tomosugi, Wataru;
Matsuda, Tomoki;
Tani, Tomomi;
Nemoto, Tomomi;
Kotera, Ippei;
Saito, Kenta;
Horikawa, Kazuki;
Nagai, Takeharu

Publication type:

Article

Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.

Published in:

Nature Methods, 2008, v. 5, n. 4, p. 339, doi. 10.1038/nmeth.1193

By:

Matsuda, Tomoki;
Miyawaki, Atsushi;
Nagai, Takeharu

Publication type:

Article

Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging.

Published in:

Journal of Biomedical Optics, 2020, v. 25, n. 1, p. 14502, doi. 10.1117/1.JBO.25.1.014502

By:

Oketani, Ryosuke;
Suda, Haruka;
Uegaki, Kumiko;
Kubo, Toshiki;
Matsuda, Tomoki;
Yamanaka, Masahito;
Arai, Yoshiyuki;
Smith, Nicholas I.;
Nagai, Takeharu;
Fujita, Katsumasa

Publication type:

Article