Works by Mielke, Thorsten

Results: 44

SecM-Stalled Ribosomes Adopt an Altered Geometry at the Peptidyl Transferase Center.

Published in:

PLoS Biology, 2011, v. 9, n. 1, p. 1, doi. 10.1371/journal.pbio.1000581

By:

Bhushan, Shashi;
Hoffmann, Thomas;
Seidelt, Birgit;
Frauenfeld, Jens;
Mielke, Thorsten;
Berninghausen, Otto;
Wilson, Daniel N.;
Beckmann, Roland

Publication type:

Article

Endothelial tip-cell position, filopodia formation and biomechanics require BMPR2 expression and signaling.

Published in:

Communications Biology, 2025, v. 8, n. 1, p. 1, doi. 10.1038/s42003-024-07431-8

By:

Hiepen, Christian;
Benamar, Mounir;
Barrasa-Fano, Jorge;
Condor, Mar;
Ilhan, Mustafa;
Münch, Juliane;
Hastar, Nurcan;
Kerkhoff, Yannic;
Harms, Gregory S.;
Mielke, Thorsten;
Koenig, Benjamin;
Block, Stephan;
Rocks, Oliver;
Abdelilah-Seyfried, Salim;
Van Oosterwyck, Hans;
Knaus, Petra

Publication type:

Article

Following the signal sequence from ribosomal tunnel exit to signal recognition particle.

Published in:

Nature, 2006, v. 444, n. 7118, p. 507, doi. 10.1038/nature05326

By:

Halic, Mario;
Blau, Michael;
Becker, Thomas;
Mielke, Thorsten;
Pool, Martin R.;
Wild, Klemens;
Sinning, Irmgard;
Beckmann, Roland

Publication type:

Article

Structure of eEF3 and the mechanism of transfer RNA release from the E-site.

Published in:

Nature, 2006, v. 443, n. 7112, p. 663, doi. 10.1038/nature05126

By:

Andersen, Christian B. F.;
Becker, Thomas;
Blau, Michael;
Anand, Monika;
Halic, Mario;
Balar, Bharvi;
Mielke, Thorsten;
Boesen, Thomas;
Pedersen, Jan Skov;
Spahn, Christian M. T.;
Kinzy, Terri Goss;
Andersen, Gregers R.;
Beckmann, Roland

Publication type:

Article

Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit.

Published in:

Nucleic Acids Research, 2011, v. 39, n. 12, p. 5264, doi. 10.1093/nar/gkr114

By:

Muhs, Margarita;
Yamamoto, Hiroshi;
Ismer, Jochen;
Takaku, Hiroaki;
Nashimoto, Masayuki;
Uchiumi, Toshio;
Nakashima, Nobuhiko;
Mielke, Thorsten;
Hildebrand, Peter W.;
Nierhaus, Knud H.;
Spahn, Christian M. T.

Publication type:

Article

Parallel Structural Evolution of Mitochondrial Ribosomes and OXPHOS Complexes.

Published in:

Genome Biology & Evolution, 2015, v. 7, n. 5, p. 1235, doi. 10.1093/gbe/evv061

By:

van der Sluis, Eli O.;
Bauerschmitt, Heike;
Becker, Thomas;
Mielke, Thorsten;
Frauenfeld, Jens;
Berninghausen, Otto;
Neupert, Walter;
Herrmann, Johannes M.;
Beckmann, Roland

Publication type:

Article

Putative Cooperative ATP–DnaA Binding to Double-Stranded DnaA Box and Single-Stranded DnaA-Trio Motif upon Helicobacter pylori Replication Initiation Complex Assembly.

Published in:

International Journal of Molecular Sciences, 2021, v. 22, n. 12, p. 6643, doi. 10.3390/ijms22126643

By:

Jaworski, Pawel;
Zyla-Uklejewicz, Dorota;
Nowaczyk-Cieszewska, Malgorzata;
Donczew, Rafal;
Mielke, Thorsten;
Weigel, Christoph;
Zawilak-Pawlik, Anna

Publication type:

Article

Structural mechanism of CRL4‐instructed STAT2 degradation via a novel cytomegaloviral DCAF receptor.

Published in:

EMBO Journal, 2023, v. 42, n. 5, p. 1, doi. 10.15252/embj.2022112351

By:

Le‐Trilling, Vu Thuy Khanh;
Banchenko, Sofia;
Paydar, Darius;
Leipe, Pia Madeleine;
Binting, Lukas;
Lauer, Simon;
Graziadei, Andrea;
Klingen, Robin;
Gotthold, Christine;
Bürger, Jörg;
Bracht, Thilo;
Sitek, Barbara;
Jan Lebbink, Robert;
Malyshkina, Anna;
Mielke, Thorsten;
Rappsilber, Juri;
Spahn, Christian MT;
Voigt, Sebastian;
Trilling, Mirko;
Schwefel, David

Publication type:

Article

Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates.

Published in:

EMBO Journal, 2022, v. 41, n. 11, p. 1, doi. 10.15252/embj.2021108882

By:

Hochmair, Janine;
Exner, Christian;
Franck, Maximilian;
Dominguez‐Baquero, Alvaro;
Diez, Lisa;
Brognaro, Hévila;
Kraushar, Matthew L;
Mielke, Thorsten;
Radbruch, Helena;
Kaniyappan, Senthilvelrajan;
Falke, Sven;
Mandelkow, Eckhard;
Betzel, Christian;
Wegmann, Susanne

Publication type:

Article

Molecular architecture of the ribosome-bound Hepatitis C Virus internal ribosomal entry site RNA.

Published in:

EMBO Journal, 2015, v. 34, n. 24, p. 3042, doi. 10.15252/embj.201592469

By:

Yamamoto, Hiroshi;
Collier, Marianne;
Loerke, Justus;
Ismer, Jochen;
Schmidt, Andrea;
Hilal, Tarek;
Sprink, Thiemo;
Yamamoto, Kaori;
Mielke, Thorsten;
Bürger, Jörg;
Shaikh, Tanvir R;
Dabrowski, Marylena;
Hildebrand, Peter W;
Scheerer, Patrick;
Spahn, Christian MT

Publication type:

Article

Cryo-EM structure of the Shigella type III needle complex.

Published in:

PLoS Pathogens, 2020, v. 16, n. 2, p. 1, doi. 10.1371/journal.ppat.1008263

By:

Lunelli, Michele;
Kamprad, Antje;
Bürger, Jörg;
Mielke, Thorsten;
Spahn, Christian M. T.;
Kolbe, Michael

Publication type:

Article

The microfollicle: a model of the human hair follicle for in vitro studies.

Published in:

In Vitro Cellular & Developmental Biology Animal, 2020, v. 56, n. 10, p. 847, doi. 10.1007/s11626-020-00513-x

By:

Ataç, Beren;
Kiss, Flora Marta;
Lam, Tobias;
Fauler, Beatrix;
Edler, Clemens;
Hu, Ping;
Tao, Thi Phuong;
Jädicke, Marian;
Rütschle, Isabel;
Azar, Reza P.;
Youngquist, Scott;
Mielke, Thorsten;
Marx, Uwe;
Lauster, Roland;
Lindner, Gerd;
DiColandrea, Teresa

Publication type:

Article

Unique and Universal Features of Epsilonproteobacterial Origins of Chromosome Replication and DnaA-DnaA Box Interactions.

Published in:

Frontiers in Microbiology, 2016, v. 7, p. 1, doi. 10.3389/fmicb.2016.01555

By:

Jaworski, Pawel;
Donczew, Rafal;
Mielke, Thorsten;
Thiel, Marcel;
Oldziej, Stanislaw;
Weigel, Christoph;
Zawilak-Pawlik, Anna

Publication type:

Article

Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM.

Published in:

Nature Communications, 2024, v. 15, n. 1, p. 1, doi. 10.1038/s41467-024-46092-3

By:

Flügel, Timo;
Schacherl, Magdalena;
Unbehaun, Anett;
Schroeer, Birgit;
Dabrowski, Marylena;
Bürger, Jörg;
Mielke, Thorsten;
Sprink, Thiemo;
Diebolder, Christoph A.;
Guillén Schlippe, Yollete V.;
Spahn, Christian M. T.

Publication type:

Article

Transient disome complex formation in native polysomes during ongoing protein synthesis captured by cryo-EM.

Published in:

Nature Communications, 2024, v. 15, n. 1, p. 1, doi. 10.1038/s41467-024-46092-3

By:

Flügel, Timo;
Schacherl, Magdalena;
Unbehaun, Anett;
Schroeer, Birgit;
Dabrowski, Marylena;
Bürger, Jörg;
Mielke, Thorsten;
Sprink, Thiemo;
Diebolder, Christoph A.;
Guillén Schlippe, Yollete V.;
Spahn, Christian M. T.

Publication type:

Article

The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles.

Published in:

Journal of Cell Biology, 2013, v. 202, n. 4, p. 667, doi. 10.1083/jcb.201301072

By:

Matkovic, Tanja;
Siebert, Matthias;
Knoche, Elena;
Depner, Harald;
Mertel, Sara;
Owald, David;
Schmidt, Manuela;
Thomas, Ulrich;
Sickmann, Albert;
Kamin, Dirk;
Hell, Stefan W.;
Bürger, Jörg;
Hollmann, Christina;
Mielke, Thorsten;
Wichmann, Carolin;
Sigrist, Stephan J.

Publication type:

Article

The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication.

Published in:

Molecular Microbiology, 2020, v. 113, n. 2, p. 338, doi. 10.1111/mmi.14423

By:

Nowaczyk‐Cieszewska, Malgorzata;
Zyla‐Uklejewicz, Dorota;
Noszka, Mateusz;
Jaworski, Pawel;
Mielke, Thorsten;
Zawilak-Pawlik, Anna Magdalena

Publication type:

Article

Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.

Published in:

Nature Communications, 2016, v. 7, n. 12, p. 13521, doi. 10.1038/ncomms13521

By:

Hilal, Tarek;
Yamamoto, Hiroshi;
Loerke, Justus;
Bürger, Jörg;
Mielke, Thorsten;
Spahn, Christian M.T.

Publication type:

Article

Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers.

Published in:

Nature Communications, 2016, v. 7, n. 10, p. 13047, doi. 10.1038/ncomms13047

By:

Arumughan, Anup;
Roske, Yvette;
Barth, Carolin;
Forero, Laura Lleras;
Bravo-Rodriguez, Kenny;
Redel, Alexandra;
Kostova, Simona;
McShane, Erik;
Opitz, Robert;
Faelber, Katja;
Rau, Kirstin;
Mielke, Thorsten;
Daumke, Oliver;
Selbach, Matthias;
Sanchez-Garcia, Elsa;
Rocks, Oliver;
Panáková, Daniela;
Heinemann, Udo;
Wanker, Erich E.

Publication type:

Article

Dual function of a highly conserved bacteriophage tail completion protein essential for bacteriophage infectivity.

Published in:

Communications Biology, 2024, v. 7, n. 1, p. 1, doi. 10.1038/s42003-024-06221-6

By:

Auzat, Isabelle;
Ouldali, Malika;
Jacquet, Eric;
Fauler, Beatrix;
Mielke, Thorsten;
Tavares, Paulo

Publication type:

Article

Visualizing the modification landscape of the human 60S ribosomal subunit at close to atomic resolution.

Published in:

Nucleic Acids Research, 2025, v. 53, n. 1, p. 1, doi. 10.1093/nar/gkae1191

By:

Wiechert, Franziska;
Unbehaun, Anett;
Sprink, Thiemo;
Seibel, Helena;
Bürger, Jörg;
Loerke, Justus;
Mielke, Thorsten;
Diebolder, Christoph A;
Schacherl, Magdalena;
Spahn, Christian M T

Publication type:

Article

Mechanistic insights into the role of prenyl-binding protein PrBP/δ in membrane dissociation of phosphodiesterase 6.

Published in:

Nature Communications, 2018, v. 9, n. 1, p. 1, doi. 10.1038/s41467-017-02569-y

By:

Qureshi, Bilal M.;
Schmidt, Andrea;
Behrmann, Elmar;
Bürger, Jörg;
Mielke, Thorsten;
Spahn, Christian M.T.;
Heck, Martin;
Scheerer, Patrick

Publication type:

Article

Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes.

Published in:

Genome Medicine, 2018, v. 10, n. 1, p. N.PAG, doi. 10.1186/s13073-018-0542-5

By:

Devulapally, Praneeth Reddy;
Bürger, Jörg;
Mielke, Thorsten;
Konthur, Zoltán;
Lehrach, Hans;
Yaspo, Marie-Laure;
Glökler, Jörn;
Warnatz, Hans-Jörg

Publication type:

Article

GTPase activation of elongation factor EF-Tu by the ribosome during decoding.

Published in:

EMBO Journal, 2009, v. 28, n. 6, p. 755, doi. 10.1038/emboj.2009.26

By:

Schuette, Jan-Christian;
Murphy, Frank V.;
Kelley, Ann C.;
Weir, John R.;
Giesebrecht, Jan;
Connell, Sean R.;
Loerke, Justus;
Mielke, Thorsten;
Wei Zhang;
Penczek, Pawel A.;
Ramakrishnan, V.;
Spahn, Christian M. T.

Publication type:

Article

Electron crystallography reveals the structure of metarhodopsin I.

Published in:

EMBO Journal, 2004, v. 23, n. 18, p. 3609, doi. 10.1038/sj.emboj.7600374

By:

Ruprecht, Jonathan J.;
Mielke, Thorsten;
Vogel, Reiner;
Villa, Claudio;
Schertler, Gebhard F. X.

Publication type:

Article

Structure of the mammalian 80S initiation complex with initiation factor 5B on HCV-IRES RNA.

Published in:

Nature Structural & Molecular Biology, 2014, v. 21, n. 8, p. 721, doi. 10.1038/nsmb.2859

By:

Yamamoto, Hiroshi;
Unbehaun, Anett;
Loerke, Justus;
Behrmann, Elmar;
Collier, Marianne;
Bürger, Jörg;
Mielke, Thorsten;
Spahn, Christian M T

Publication type:

Article

Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome.

Published in:

Nature Structural & Molecular Biology, 2011, v. 18, n. 6, p. 715, doi. 10.1038/nsmb.2057

By:

Becker, Thomas;
Armache, Jean-Paul;
Jarasch, Alexander;
Anger, Andreas M.;
Villa, Elizabeth;
Sieber, Heidemarie;
Motaal, Basma Abdel;
Mielke, Thorsten;
Berninghausen, Otto;
Beckmann, Roland

Publication type:

Article

Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.

Published in:

Nature Structural & Molecular Biology, 2011, v. 18, n. 5, p. 614, doi. 10.1038/nsmb.2026

By:

Frauenfeld, Jens;
Gumbart, James;
Sluis, Eli O van der;
Funes, Soledad;
Gartmann, Marco;
Beatrix, Birgitta;
Mielke, Thorsten;
Berninghausen, Otto;
Becker, Thomas;
Schulten, Klaus;
Beckmann, Roland

Publication type:

Article

α-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel.

Published in:

Nature Structural & Molecular Biology, 2010, v. 17, n. 3, p. 313, doi. 10.1038/nsmb.1756

By:

Bhushan, Shashi;
Gartmann, Marco;
Halic, Mario;
Armache, Jean-Paul;
Jarasch, Alexander;
Mielke, Thorsten;
Berninghausen, Otto;
Wilson, Daniel N.;
Beckmann, Roland

Publication type:

Article

A new tRNA intermediate revealed on the ribosome during EF4-mediated back-translocation.

Published in:

Nature Structural & Molecular Biology, 2008, v. 15, n. 9, p. 910, doi. 10.1038/nsmb.1469

By:

Connell, Sean R.;
Topf, Maya;
Yan Qin;
Wilson, Daniel N.;
Mielke, Thorsten;
Fucini, Paola;
Nierhaus, Knud H.;
Spahn, Christian M. T.

Publication type:

Article

Structure of the ribosome-bound cricket paralysis virus IRES RNA.

Published in:

Nature Structural & Molecular Biology, 2006, v. 13, n. 12, p. 1092, doi. 10.1038/nsmb1177

By:

Schüler, Martin;
Connell, Sean R.;
Lescoute, Aurelie;
Giesebrecht, Jan;
Dabrowski, Marylena;
Schroeer, Birgit;
Mielke, Thorsten;
Penczek, Pawel A.;
Westhof, Eric;
Spahn, Christian M. T.

Publication type:

Article

Electron Crystallographic Studies of Rhodopsin.

Published in:

Phase Transitions, 2002, v. 75, n. 1/2, p. 1, doi. 10.1080/01411590290022888

By:

Ruprecht, Jonathan;
Mielke, Thorsten;
Krebs, Angelika;
Schertler, Gebhard

Publication type:

Article

T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation.

Published in:

eLife, 2023, p. 1, doi. 10.7554/eLife.83077

By:

Landshammer, Alexandro;
Bolondi, Adriano;
Kretzmer, Helene;
Much, Christian;
Buschow, René;
Rose, Alina;
Hua-Jun;
Mackowiak, Sebastian D.;
Braendl, Bjoern;
Giesselmann, Pay;
Tornisiello, Rosaria;
Parsi, Krishna Mohan;
Huey, Jack;
Mielke, Thorsten;
Meierhofer, David;
Maehr, René;
Hnisz, Denes;
Michor, Franziska;
Rinn, John L.;
Meissner, Alexander

Publication type:

Article

Structure and Function of the <italic>Campylobacter jejuni</italic> Chromosome Replication Origin.

Published in:

Frontiers in Microbiology, 2018, p. N.PAG, doi. 10.3389/fmicb.2018.01533

By:

Jaworski, Pawel;
Donczew, Rafal;
Mielke, Thorsten;
Weigel, Christoph;
Stingl, Kerstin;
Zawilak-Pawlik, Anna

Publication type:

Article

Unc13A and Unc13B contribute to the decoding of distinct sensory information in Drosophila.

Published in:

Nature Communications, 2021, v. 12, n. 1, p. 1, doi. 10.1038/s41467-021-22180-6

By:

Pooryasin, Atefeh;
Maglione, Marta;
Schubert, Marco;
Matkovic-Rachid, Tanja;
Hasheminasab, Sayed-mohammad;
Pech, Ulrike;
Fiala, André;
Mielke, Thorsten;
Sigrist, Stephan J.

Publication type:

Article

Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase.

Published in:

Nature Communications, 2020, v. 11, n. 1, p. 1, doi. 10.1038/s41467-020-15614-0

By:

Radon, Christin;
Mittelstädt, Gerd;
Duffus, Benjamin R.;
Bürger, Jörg;
Hartmann, Tobias;
Mielke, Thorsten;
Teutloff, Christian;
Leimkühler, Silke;
Wendler, Petra

Publication type:

Article

The CoxD Protein, a Novel AAA+ ATPase Involved in Metal Cluster Assembly: Hydrolysis of Nucleotide- Triphosphates and Oligomerization.

Published in:

PLoS ONE, 2012, v. 7, n. 10, p. 1, doi. 10.1371/journal.pone.0047424

By:

Maisel, Tobias;
Joseph, Stephanie;
Mielke, Thorsten;
Bürger, Jörg;
Schwarzinger, Stephan;
Meyer, Ortwin

Publication type:

Article

The complex of tmRNA-SmpB and EF-G on translocating ribosomes.

Published in:

Nature, 2012, v. 485, n. 7399, p. 526, doi. 10.1038/nature11006

By:

Ramrath, David J. F.;
Yamamoto, Hiroshi;
Rother, Kristian;
Wittek, Daniela;
Pech, Markus;
Mielke, Thorsten;
Loerke, Justus;
Scheerer, Patrick;
Ivanov, Pavel;
Teraoka, Yoshika;
Shpanchenko, Olga;
Nierhaus, Knud H.;
Spahn, Christian M. T.

Publication type:

Article

Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites.

Published in:

Nature, 2010, v. 468, n. 7324, p. 713, doi. 10.1038/nature09547

By:

Ratje, Andreas H.;
Loerke, Justus;
Mikolajka, Aleksandra;
Brünner, Matthias;
Hildebrand, Peter W.;
Starosta, Agata L.;
Dönhöfer, Alexandra;
Connell, Sean R.;
Fucini, Paola;
Mielke, Thorsten;
Whitford, Paul C.;
Onuchic, José N.;
Yanan Yu;
Sanbonmatsu, Karissa Y.;
Hartmann, Roland K.;
Penczek, Pawel A.;
Wilson, Daniel N.;
Spahn, Christian M. T.

Publication type:

Article

Coupled chaperone action in folding and assembly of hexadecameric Rubisco.

Published in:

Nature, 2010, v. 463, n. 7278, p. 197, doi. 10.1038/nature08651

By:

Cuimin Liu;
Young, Anna L.;
Starling-Windhof, Amanda;
Bracher, Andreas;
Saschenbrecker, Sandra;
Rao, Bharathi Vasudeva;
Rao, Karnam Vasudeva;
Berninghausen, Otto;
Mielke, Thorsten;
Hartl, F. Ulrich;
Beckmann, Roland;
Hayer-Hartl, Manajit

Publication type:

Article

Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses.

Published in:

PLoS Pathogens, 2021, v. 17, n. 8, p. 1, doi. 10.1371/journal.ppat.1009775

By:

Banchenko, Sofia;
Krupp, Ferdinand;
Gotthold, Christine;
Bürger, Jörg;
Graziadei, Andrea;
O'Reilly, Francis J.;
Sinn, Ludwig;
Ruda, Olga;
Rappsilber, Juri;
Spahn, Christian M. T.;
Mielke, Thorsten;
Taylor, Ian A.;
Schwefel, David

Publication type:

Article

Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release.

Published in:

PLoS Biology, 2016, v. 14, n. 9, p. 1, doi. 10.1371/journal.pbio.1002563

By:

Gupta, Varun K.;
Pech, Ulrike;
Bhukel, Anuradha;
Fulterer, Andreas;
Ender, Anatoli;
Mauermann, Stephan F.;
Andlauer, Till F. M.;
Antwi-Adjei, Emmanuel;
Beuschel, Christine;
Thriene, Kerstin;
Maglione, Marta;
Quentin, Christine;
Bushow, René;
Schwärzel, Martin;
Mielke, Thorsten;
Madeo, Frank;
Dengjel, Joern;
Fiala, André;
Sigrist, Stephan J.

Publication type:

Article

Structural transitions during the scaffolding-driven assembly of a viral capsid.

Published in:

Nature Communications, 2019, v. 10, n. 1, p. 1, doi. 10.1038/s41467-019-12790-6

By:

Ignatiou, Athanasios;
Brasilès, Sandrine;
El Sadek Fadel, Mehdi;
Bürger, Jörg;
Mielke, Thorsten;
Topf, Maya;
Tavares, Paulo;
Orlova, Elena V.

Publication type:

Article

Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium.

Published in:

Nature Communications, 2019, v. 10, n. 1, p. N.PAG, doi. 10.1038/s41467-019-10954-y

By:

Brandt, Raphael;
Sell, Thomas;
Lüthen, Mareen;
Uhlitz, Florian;
Klinger, Bertram;
Riemer, Pamela;
Giesecke-Thiel, Claudia;
Schulze, Silvia;
El-Shimy, Ismail Amr;
Kunkel, Desiree;
Fauler, Beatrix;
Mielke, Thorsten;
Mages, Norbert;
Herrmann, Bernhard G.;
Sers, Christine;
Blüthgen, Nils;
Morkel, Markus

Publication type:

Article