Works by Liu, Zuohua

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    Identification of Single Nucleotide Polymorphisms Through Genome-Wide Association Studies of pH Traits in Goose Meat The genetic regulation of goose meat quality traits remains relatively unexplored, and the underlying mechanisms are yet to be elucidated. This study aims to employ single nucleotide polymorphism (SNP) genotyping in conjunction with genome-wide association studies (GWAS) to investigate critical candidate regions and genes associated with the pH trait of meat in Sichuan white geese. A cohort of 203 healthy male Sichuan white geese was randomly selected and slaughtered at 70 days of age. Measurements were taken of meat pH, growth parameters, body dimensions, and post-slaughter traits. High-throughput sequencing on the Illumina HiSeq X Ten platform facilitated gene resequencing and SNP evaluation, and GWAS was employed to detect key genes within quantitative trait loci (QTL) intervals. The sequencing of 203 individuals yielded a total of 2601.19 Gb of genomic data, with an average sequencing depth of 10.89×. Through GWAS analysis, a total of 30 SNPs associated with pH were identified. These SNPs were identified on multiple chromosomes, including on chromosome 17 (chr: 23.57–23.68 Mb) and chromosome 13 (chr13: 31.52–31.61 Mb). By annotating these associated SNPs, nine candidate genes (including C19L2, AMFR, POL, RERGL, ZN484, GMDS, WAC) associated with the pH of goose meat were identified. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) genotyping of 10 SNPs centered on these nine candidate genes was confirmed. GO enrichment analysis revealed that genes within 1 Mb of the associated SNPs are significantly enriched in pathways involved in lymphocyte activation, in response to hydrogen peroxide, Salmonella infection, and other metabolic processes. This study explores the gene regulatory pathways influencing pH traits in goose meat and provides molecular markers for enhancing meat quality. These f

    Published in:
    Biology (2079-7737), 2024, v. 13, n. 11, p. 865, doi. 10.3390/biology13110865
    By:
    • Wang, Haiwei;
    • Chen, Zhuping;
    • Ma, Lin;
    • Wu, Yifan;
    • Zhao, Xianzhi;
    • Zhang, Keshan;
    • Xue, Jiajia;
    • Luo, Yi;
    • Wang, Chao;
    • Liu, Zuohua;
    • Xie, Youhui;
    • Chen, Ying;
    • Gao, Guangliang;
    • Wang, Qigui
    Publication type:
    Article