Works by Kolmar, Harald

Results: 151

A Recognition Tag of Human Origin for Bioorthogonal Generation of Antibody‐Drug Conjugates using Microbial Biotin Ligase.

Published in:

ChemBioChem, 2025, v. 26, n. 10, p. 1, doi. 10.1002/cbic.202500261

By:

Bitsch, Peter;
Bitsch, Sebastian;
Murmann, Noah;
Bork, Ingo;
Becker, Janine;
Kolmar, Harald

Publication type:

Article

Selection of Antibodies with Tailored Properties by Application of High-Throughput Multiparameter Fluorescence-Activated Cell Sorting of Yeast-Displayed Immune Libraries.

Published in:

Molecular Biotechnology, 2018, v. 60, n. 10, p. 727, doi. 10.1007/s12033-018-0109-0

By:

Schröter, Christian;
Beck, Jan;
Krah, Simon;
Zielonka, Stefan;
Doerner, Achim;
Rhiel, Laura;
Günther, Ralf;
Toleikis, Lars;
Kolmar, Harald;
Hock, Björn;
Becker, Stefan

Publication type:

Article

Protein Production in Yarrowia lipolytica Via Fusion to the Secreted Lipase Lip2p.

Published in:

Molecular Biotechnology, 2014, v. 56, n. 1, p. 79, doi. 10.1007/s12033-013-9684-2

By:

Hofmeyer, Thomas;
Bulani, Siyavuya;
Grzeschik, Julius;
Krah, Simon;
Glotzbach, Bernhard;
Uth, Christina;
Avrutina, Olga;
Brecht, Michael;
Göringer, Hans;
Zyl, Petrus;
Kolmar, Harald

Publication type:

Article

Ein Apoptose-induzierendes Heptamer, das effizient den Todesrezeptor 5 bündelt.

Published in:

Angewandte Chemie, 2016, v. 128, n. 16, p. 5169, doi. 10.1002/ange.201511894

By:

Valldorf, Bernhard;
Fittler, Heiko;
Deweid, Lukas;
Ebenig, Aileen;
Dickgiesser, Stephan;
Sellmann, Carolin;
Becker, Janine;
Zielonka, Stefan;
Empting, Martin;
Avrutina, Olga;
Kolmar, Harald

Publication type:

Article

Durch Design verbrückt: ein konformativ eingeschränkter Transglutaminase-Marker ermöglicht effiziente ortsspezifische Konjugation.

Published in:

Angewandte Chemie, 2015, v. 127, n. 45, p. 13618, doi. 10.1002/ange.201504851

By:

Siegmund, Vanessa;
Schmelz, Stefan;
Dickgiesser, Stephan;
Beck, Jan;
Ebenig, Aileen;
Fittler, Heiko;
Frauendorf, Holm;
Piater, Birgit;
Betz, Ulrich A. K.;
Avrutina, Olga;
Scrima, Andrea;
Fuchsbauer, Hans‐Lothar;
Kolmar, Harald

Publication type:

Article

Effektive Markierung von bioaktiven Peptiden mit PHIP-Markern zur Steigerung der Empfindlichkeit von NMR-Signalen.

Published in:

Angewandte Chemie, 2014, v. 126, n. 47, p. 13155, doi. 10.1002/ange.201404668

By:

Sauer, Grit;
Nasu, Daichi;
Tietze, Daniel;
Gutmann, Torsten;
Englert, Simon;
Avrutina, Olga;
Kolmar, Harald;
Buntkowsky, Gerd

Publication type:

Article

Eine chemoenzymatische Kupplungsstrategie zur Immobilisierung von Proteinen auf kristalliner Nanocellulose.

Published in:

Angewandte Chemie, 2014, v. 126, n. 46, p. 12826, doi. 10.1002/ange.201404616

By:

Uth, Christina;
Zielonka, Stefan;
Hörner, Sebastian;
Rasche, Nicolas;
Plog, Andreas;
Orelma, Hannes;
Avrutina, Olga;
Kai Zhang;
Kolmar, Harald

Publication type:

Article

Peptid in Ketten: Einblicke in die Struktur-Aktivitäts-Beziehungen von Proteaseinhibitormimetika mit fixierten Amidkonformationen.

Published in:

Angewandte Chemie, 2012, v. 124, n. 15, p. 3768, doi. 10.1002/ange.201108983

By:

Tischler, Marco;
Nasu, Daichi;
Empting, Martin;
Schmelz, Stefan;
Heinz, Dirk W.;
Rottmann, Philipp;
Kolmar, Harald;
Buntkowsky, Gerd;
Tietze, Daniel;
Avrutina, Olga

Publication type:

Article

'Triazolbrücke': ein Disulfidbrückenersatz durch Ruthenium- katalysierte Bildung von 1,5-disubstituierten 1,2,3-Triazolen.

Published in:

Angewandte Chemie, 2011, v. 123, n. 22, p. 5313, doi. 10.1002/ange.201008142

By:

Empting, Martin;
Avrutina, Olga;
Meusinger, Reinhard;
Fabritz, Sebastian;
Reinwarth, Michael;
Biesalski, Markus;
Voigt, Stephan;
Buntkowsky, Gerd;
Kolmar, Harald

Publication type:

Article

Dissecting capture and twisting of aureolysin and pseudolysin: functional amino acids of the Dispase autolysis-inducing protein.

Published in:

Biochemical Journal, 2020, v. 477, n. 13, p. 2595, doi. 10.1042/BCJ20200407

By:

Fiebig, David;
Anderl, Anita;
Djaizani, Sahra Al;
Kolmar, Harald;
Fuchsbauer, Hans-Lothar

Publication type:

Article

Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post‐Assembly Loading.

Published in:

Advanced Biology, 2025, v. 9, n. 5, p. 1, doi. 10.1002/adbi.202400483

By:

Belluati, Andrea;
Bloch, Adrian;
Koynov, Kaloian;
Müller Nieva, Mariana;
Bagherabadi, Mohadeseh;
Andrieu‐Brunsen, Annette;
Kolmar, Harald;
Bruns, Nico

Publication type:

Article

Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells.

Published in:

Communications Biology, 2022, v. 5, n. 1, p. 1, doi. 10.1038/s42003-022-03604-5

By:

Kubitz, Lea;
Bitsch, Sebastian;
Zhao, Xiyan;
Schmitt, Kerstin;
Deweid, Lukas;
Roehrig, Amélie;
Barazzone, Elisa Cappio;
Valerius, Oliver;
Kolmar, Harald;
Béthune, Julien

Publication type:

Article

Dual Function pH Responsive Bispecific Antibodies for Tumor Targeting and Antigen Depletion in Plasma.

Published in:

Frontiers in Immunology, 2019, p. N.PAG, doi. 10.3389/fimmu.2019.01892

By:

Bogen, Jan P.;
Hinz, Steffen C.;
Grzeschik, Julius;
Ebenig, Aileen;
Krah, Simon;
Zielonka, Stefan;
Kolmar, Harald

Publication type:

Article

Single-Cell High-Throughput Screening To Identify Enantioselective Hydrolytic Enzymes.

Published in:

Angewandte Chemie International Edition, 2008, v. 47, n. 27, p. 5085, doi. 10.1002/anie.200705236

By:

Becker, Stefan;
Höbenreich, Horst;
Vogel, Andreas;
Knorr, Janina;
Wilhelm, Susanne;
Rosenau, Frank;
Jaeger, Karl-Erich;
Reetz, Manfred T.;
Kolmar, Harald

Publication type:

Article

Cover Feature: Ultrafast Single‐Scan 2D NMR Spectroscopic Detection of a PHIP‐Hyperpolarized Protease Inhibitor (Chem. Eur. J. 16/2019).

Published in:

Chemistry - A European Journal, 2019, v. 25, n. 16, p. 3966, doi. 10.1002/chem.201900875

By:

Kiryutin, Alexey S.;
Sauer, Grit;
Tietze, Daniel;
Brodrecht, Martin;
Knecht, Stephan;
Yurkovskaya, Alexandra V.;
Ivanov, Konstantin L.;
Avrutina, Olga;
Kolmar, Harald;
Buntkowsky, Gerd

Publication type:

Article

Ultrafast Single‐Scan 2D NMR Spectroscopic Detection of a PHIP‐Hyperpolarized Protease Inhibitor.

Published in:

Chemistry - A European Journal, 2019, v. 25, n. 16, p. 4025, doi. 10.1002/chem.201900079

By:

Kiryutin, Alexey S.;
Sauer, Grit;
Tietze, Daniel;
Brodrecht, Martin;
Knecht, Stephan;
Yurkovskaya, Alexandra V.;
Ivanov, Konstantin L.;
Avrutina, Olga;
Kolmar, Harald;
Buntkowsky, Gerd

Publication type:

Article

Light‐Controlled Chemoenzymatic Immobilization of Proteins towards Engineering of Bioactive Papers.

Published in:

Chemistry - A European Journal, 2019, v. 25, n. 7, p. 1746, doi. 10.1002/chem.201804889

By:

Hilberg, Valentina;
Avrutina, Olga;
Ebenig, Aileen;
Yanakieva, Desislava;
Meckel, Tobias;
Biesalski, Markus;
Kolmar, Harald

Publication type:

Article

Directed Evolution of a Bond‐Forming Enzyme: Ultrahigh‐Throughput Screening of Microbial Transglutaminase Using Yeast Surface Display.

Published in:

Chemistry - A European Journal, 2018, v. 24, n. 57, p. 15195, doi. 10.1002/chem.201803485

By:

Deweid, Lukas;
Neureiter, Lara;
Englert, Simon;
Schneider, Hendrik;
Deweid, Jakob;
Yanakieva, Desislava;
Sturm, Janna;
Bitsch, Sebastian;
Christmann, Andreas;
Avrutina, Olga;
Fuchsbauer, Hans‐Lothar;
Kolmar, Harald

Publication type:

Article

Required polymer lengths per precipitated protein molecule in protein-polymer interaction.

Published in:

Journal of Polymer Research, 2014, v. 21, n. 2, p. 1, doi. 10.1007/s10965-013-0346-7

By:

Capito, Florian;
Kolmar, Harald;
Stanislawski, Bernd;
Skudas, Romas

Publication type:

Article

Bispecific killer cell engagers employing species crossreactive NKG2D binders redirect human and murine lymphocytes to ErbB2/HER2- positive malignancies.

Published in:

Frontiers in Immunology, 2024, p. 1, doi. 10.3389/fimmu.2024.1457887

By:

Serrahima, Jordi Pfeifer;
Schoenfeld, Katrin;
Kühnel, Ines;
Harwardt, Julia;
Palacios, Arturo Macarrón;
Prüfer, Maren;
Kolaric, Margareta;
Oberoi, Pranav;
Kolmar, Harald;
Wels, Winfried S.

Publication type:

Article

Using protein geometry to optimize cytotoxicity and the cytokine window of a ROR1 specific T cell engager.

Published in:

Frontiers in Immunology, 2024, p. 01, doi. 10.3389/fimmu.2024.1323049

By:

Xueyuan Zhou;
Geyer, Felix Klaus;
Happel, Dominic;
Takimoto, Jeffrey;
Kolmar, Harald;
Rabinovich, Brian

Publication type:

Article

Cattle-derived knob paratopes grafted onto peripheral loops of the IgG1 Fc region enable the generation of a novel symmetric bispecific antibody format.

Published in:

Frontiers in Immunology, 2023, p. 1, doi. 10.3389/fimmu.2023.1238313

By:

Yanakieva, Desislava;
Vollmer, Lena;
Evers, Andreas;
Siegmund, Vanessa;
Arras, Paul;
Pekar, Lukas;
Doerner, Achim;
Valldorf, Bernhard;
Kolmar, Harald;
Zielonka, Stefan;
Krah, Simon

Publication type:

Article

Cattle-derived knob paratopes grafted onto peripheral loops of the IgG1 Fc region enable the generation of a novel symmetric bispecific antibody format.

Published in:

Frontiers in Immunology, 2023, p. 01, doi. 10.3389/fimmu.2023.1238313

By:

Yanakieva, Desislava;
Vollmer, Lena;
Evers, Andreas;
Siegmund, Vanessa;
Arras, Paul;
Pekar, Lukas;
Doerner, Achim;
Valldorf, Bernhard;
Kolmar, Harald;
Zielonka, Stefan;
Krah, Simon

Publication type:

Article

Novel amino‐Li resin for water‐based solid‐phase peptide synthesis.

Published in:

Journal of Peptide Science, 2023, v. 29, n. 12, p. 1, doi. 10.1002/psc.3527

By:

Uth, Christina;
Englert, Simon;
Avrutina, Olga;
Kolmar, Harald;
Knauer, Sascha

Publication type:

Article

Multivalent dextran hybrids for efficient cytosolic delivery of biomolecular cargoes.

Published in:

Journal of Peptide Science, 2021, v. 27, n. 4, p. 1, doi. 10.1002/psc.3298

By:

Becker, Bastian;
Englert, Simon;
Schneider, Hendrik;
Yanakieva, Desislava;
Hofmann, Sarah;
Dombrowsky, Carolin;
Macarrón Palacios, Arturo;
Bitsch, Sebastian;
Elter, Adrian;
Meckel, Tobias;
Kugler, Benedikt;
Schirmacher, Anastasyia;
Avrutina, Olga;
Diederichsen, Ulf;
Kolmar, Harald

Publication type:

Article

Cystine-knot peptides targeting cancer-relevant human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4).

Published in:

Journal of Peptide Science, 2015, v. 21, n. 8, p. 651, doi. 10.1002/psc.2782

By:

Maaß, Franziska;
Wüstehube‐Lausch, Joycelyn;
Dickgießer, Stephan;
Valldorf, Bernhard;
Reinwarth, Michael;
Schmoldt, Hans‐Ulrich;
Daneschdar, Matin;
Avrutina, Olga;
Sahin, Ugur;
Kolmar, Harald

Publication type:

Article

Potent inhibitors of human matriptase-1 based on the scaffold of sunflower trypsin inhibitor.

Published in:

Journal of Peptide Science, 2014, v. 20, n. 6, p. 415, doi. 10.1002/psc.2629

By:

Fittler, Heiko;
Avrutina, Olga;
Empting, Martin;
Kolmar, Harald

Publication type:

Article

REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells.

Published in:

PLoS ONE, 2014, v. 9, n. 12, p. 1, doi. 10.1371/journal.pone.0114887

By:

Rhiel, Laura;
Krah, Simon;
Günther, Ralf;
Becker, Stefan;
Kolmar, Harald;
Hock, Björn

Publication type:

Article

Fragmentation Follows Structure: Top-Down Mass Spectrometry Elucidates the Topology of Engineered Cystine-Knot Miniproteins.

Published in:

PLoS ONE, 2014, v. 9, n. 10, p. 1, doi. 10.1371/journal.pone.0108626

By:

Reinwarth, Michael;
Avrutina, Olga;
Fabritz, Sebastian;
Kolmar, Harald

Publication type:

Article

Combinatorial Optimization of Cystine-Knot Peptides towards High-Affinity Inhibitors of Human Matriptase-1.

Published in:

PLoS ONE, 2013, v. 8, n. 10, p. 1, doi. 10.1371/journal.pone.0076956

By:

Glotzbach, Bernhard;
Reinwarth, Michael;
Weber, Niklas;
Fabritz, Sebastian;
Tomaszowski, Michael;
Fittler, Heiko;
Christmann, Andreas;
Avrutina, Olga;
Kolmar, Harald

Publication type:

Article

Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line.

Published in:

Biotechnology Progress, 2023, v. 39, n. 5, p. 1, doi. 10.1002/btpr.3372

By:

Moisant, Rémy;
Cowles, Elliott;
Broutel, Léonie;
Deparis, Véronique;
Baud, Anthony;
von Hagen, Joerg;
Kolmar, Harald

Publication type:

Article

Generation of a host cell line containing a MAR‐rich landing pad for site‐specific integration and expression of transgenes.

Published in:

Biotechnology Progress, 2022, v. 38, n. 4, p. 1, doi. 10.1002/btpr.3254

By:

Oliviero, Claudia;
Hinz, Steffen C.;
Bogen, Jan P.;
Kornmann, Henri;
Hock, Björn;
Kolmar, Harald;
Hagens, Gerrit

Publication type:

Article

Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line.

Published in:

Biotechnology Progress, 2021, v. 37, n. 4, p. 1, doi. 10.1002/btpr.3147

By:

Djemal, Leïla;
von Hagen, Joerg;
Kolmar, Harald;
Deparis, Véronique

Publication type:

Article

Review: High temperature short time treatment of cell culture media and feed solutions to mitigate adventitious viral contamination in the biopharmaceutical industry.

Published in:

Biotechnology Progress, 2021, v. 37, n. 3, p. 1, doi. 10.1002/btpr.3117

By:

Djemal, Leïla;
Fournier, Clemence;
Hagen, Joerg;
Kolmar, Harald;
Deparis, Véronique

Publication type:

Article

Trypsin inhibition by macrocyclic and open-chain variants of the squash inhibitor MCoTI-II.

Published in:

Biological Chemistry, 2005, v. 386, n. 12, p. 1301, doi. 10.1515/BC.2005.148

By:

Avrutina, Olga;
Schmoldt, Hans-Ulrich;
Gabrijelcic-Geiger, Dusica;
Dung Le Nguyen;
Sommerhoff, Christian P.;
Diederichsen, Ulf;
Kolmar, Harald

Publication type:

Article

A Soluble Immunoglobulin Variable Domain without a Disulfide Bridge: Construction, Accumulation in the Cytoplasm of E. coli, Purification and Physicochemical Characterization.

Published in:

Biological Chemistry, 1994, v. 375, n. 5, p. 353

By:

Frisch, Christian;
Kolmar, Harald;
Fritz, Hans-Joachim

Publication type:

Article

Dimerization of Bence Jones Proteins: Linking the Rate of Transcription from an Escherichia coli Promoter to the Association Constant of REI<sub>V</sub>.

Published in:

Biological Chemistry, 1994, v. 375, n. 1, p. 61

By:

Kolmar, Harald;
Frisch, Christian;
Kleemann, Gerd;
Götze, Kerstin;
Stevens, Fred J.;
Fritz, Hans-Joachim

Publication type:

Article

A Conditionally Activated Cytosol-Penetrating Antibody for TME-Dependent Intracellular Cargo Delivery.

Published in:

Antibodies (2073-4468), 2024, v. 13, n. 2, p. 37, doi. 10.3390/antib13020037

By:

Dombrowsky, Carolin Sophie;
Happel, Dominic;
Habermann, Jan;
Hofmann, Sarah;
Otmi, Sasi;
Cohen, Benny;
Kolmar, Harald

Publication type:

Article

Balancing the Affinity and Tumor Cell Binding of a Two-in-One Antibody Simultaneously Targeting EGFR and PD-L1.

Published in:

Antibodies (2073-4468), 2024, v. 13, n. 2, p. 36, doi. 10.3390/antib13020036

By:

Harwardt, Julia;
Geyer, Felix Klaus;
Schoenfeld, Katrin;
Baumstark, David;
Molkenthin, Vera;
Kolmar, Harald

Publication type:

Article

Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System.

Published in:

Antibodies (2073-4468), 2021, v. 10, n. 2, p. 18, doi. 10.3390/antib10020018

By:

Carrara, Stefania C.;
Fiebig, David;
Bogen, Jan P.;
Grzeschik, Julius;
Hock, Björn;
Kolmar, Harald

Publication type:

Article

Impact of Acetylated and Non-Acetylated Fucose Analogues on IgG Glycosylation.

Published in:

Antibodies (2073-4468), 2019, v. 8, n. 1, p. 9, doi. 10.3390/antib8010009

By:

Zimmermann, Martina;
Ehret, Janike;
Kolmar, Harald;
Zimmer, Aline

Publication type:

Article

Engineering IgG-Like Bispecific Antibodies—An Overview.

Published in:

Antibodies (2073-4468), 2018, v. 7, n. 3, p. 28, doi. 10.3390/antib7030028

By:

Krah, Simon;
Kolmar, Harald;
Becker, Stefan;
Zielonka, Stefan

Publication type:

Article

Discovery of potent allosteric antibodies inhibiting EGFR.

Published in:

2024

By:

Fournier, Léxane;
Pekar, Lukas;
Leuthner, Birgitta;
Kolmar, Harald;
Toleikis, Lars;
Becker, Stefan

Publication type:

Report

Generation and engineering of potent single domain antibody-based bispecific IL-18 mimetics resistant to IL-18BP decoy receptor inhibition.

Published in:

2023

By:

Lipinski, Britta;
Unmuth, Laura;
Arras, Paul;
Becker, Stefan;
Bauer, Christina;
Toleikis, Lars;
Krah, Simon;
Doerner, Achim;
Yanakieva, Desislava;
Boje, Ammelie Svea;
Klausz, Katja;
Peipp, Matthias;
Siegmund, Vanessa;
Evers, Andreas;
Kolmar, Harald;
Pekar, Lukas;
Zielonka, Stefan

Publication type:

Report

Beyond bispecificity: Controlled Fab arm exchange for the generation of antibodies with multiple specificities.

Published in:

2022

By:

Yanakieva, Desislava;
Pekar, Lukas;
Evers, Andreas;
Fleischer, Markus;
Keller, Stephan;
Mueller-Pompalla, Dirk;
Toleikis, Lars;
Kolmar, Harald;
Zielonka, Stefan;
Krah, Simon

Publication type:

Report

Intein mediated high throughput screening for bispecific antibodies.

Published in:

2020

By:

Hofmann, Tim;
Schmidt, Johannes;
Ciesielski, Elke;
Becker, Stefan;
Rysiok, Thomas;
Schütte, Mark;
Toleikis, Lars;
Kolmar, Harald;
Doerner, Achim

Publication type:

Report

Structural insights and biomedical potential of IgNAR scaffolds from sharks.

Published in:

mAbs (1942-0862), 2015, v. 7, n. 1, p. 15, doi. 10.4161/19420862.2015.989032

By:

Zielonka, Stefan;
Empting, Martin;
Grzeschik, Julius;
Könning, Doreen;
Barelle, Caroline J;
Kolmar, Harald

Publication type:

Article

A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display.

Published in:

mAbs (1942-0862), 2015, v. 7, n. 1, p. 138, doi. 10.4161/19420862.2014.985993

By:

Schröter, Christian;
Günther, Ralf;
Rhiel, Laura;
Becker, Stefan;
Toleikis, Lars;
Doerner, Achim;
Becker, Janine;
Schönemann, Andreas;
Nasu, Daichi;
Neuteboom, Berend;
Kolmar, Harald;
Hock, Björn

Publication type:

Article

Fmoc-Assisted Synthesis of a 29-Residue Cystine-Knot Trypsin Inhibitor Containing a Guaninyl Amino Acid at the P1-Position.

Published in:

European Journal of Organic Chemistry, 2004, v. 2004, n. 23, p. 4931, doi. 10.1002/ejoc.200400440

By:

Olga Avrutina;
Hans-Ulrich Schmoldt;
Harald Kolmar;
Ulf Diederichsen

Publication type:

Article

Sactipeptide Engineering by Probing the Substrate Tolerance of a Thioether‐Bond‐Forming Sactisynthase.

Published in:

Angewandte Chemie International Edition, 2022, v. 61, n. 45, p. 1, doi. 10.1002/anie.202210883

By:

Ali, Ataurehman;
Happel, Dominic;
Habermann, Jan;
Schoenfeld, Katrin;
Macarrón Palacios, Arturo;
Bitsch, Sebastian;
Englert, Simon;
Schneider, Hendrik;
Avrutina, Olga;
Fabritz, Sebastian;
Kolmar, Harald

Publication type:

Article