Works by Keasling, Jay D.

Results: 204

Merging the computational design of chimeric type I polyketide synthases with enzymatic pathways for chemical biosynthesis.

Published in:

Nature Communications, 2025, v. 16, n. 1, p. 1, doi. 10.1038/s41467-025-61160-y

By:

Chainani, Yash;
Diaz, Jacob;
Guilarte-Silva, Margaret;
Blay, Vincent;
Zhang, Quan;
Sprague, William;
Tyo, Keith E. J.;
Broadbelt, Linda J.;
Mukhopadhyay, Aindrila;
Keasling, Jay D.;
Martin, Hector Garcia;
Backman, Tyler W. H.

Publication type:

Article

Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli.

Published in:

Nature Communications, 2025, v. 16, n. 1, p. 1, doi. 10.1038/s41467-025-61001-y

By:

Cowan, Aidan E.;
Hillers, Mason;
Rainaldi, Vittorio;
Collas, Florent;
Choudhary, Hemant;
Zakaria, Basem S.;
Bieberach, Gregory G.;
Carruthers, David N.;
Grabovac, Maxwell;
Gin, Jennifer W.;
Cawthon, Bridgie;
Chen, Yan;
Turumtay, Emine Akyuz;
Baidoo, Edward E. K.;
Petzold, Christopher J.;
Feist, Adam M.;
Tejedor-Sanz, Sara;
Kensy, Frank;
Simmons, Blake A.;
Keasling, Jay D.

Publication type:

Article

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development.

Published in:

Nature Reviews Microbiology, 2014, v. 12, n. 5, p. 355, doi. 10.1038/nrmicro3240

By:

Paddon, Chris J.;
Keasling, Jay D.

Publication type:

Article

Insight into the Mechanism of Phenylacetate Decarboxylase (PhdB), a Toluene‐Producing Glycyl Radical Enzyme.

Published in:

ChemBioChem, 2020, v. 21, n. 5, p. 663, doi. 10.1002/cbic.201900560

By:

Rodrigues, Andria V.;
Tantillo, Dean J.;
Mukhopadhyay, Aindrila;
Keasling, Jay D.;
Beller, Harry R.

Publication type:

Article

Cover Feature: Biochemical Characterization of β‐Amino Acid Incorporation in Fluvirucin B<sub>2</sub> Biosynthesis (ChemBioChem 13/2018).

Published in:

ChemBioChem, 2018, v. 19, n. 13, p. 1351, doi. 10.1002/cbic.201800299

By:

Barajas, Jesus F.;
Zargar, Amin;
Pang, Bo;
Benites, Veronica T.;
Gin, Jennifer;
Baidoo, Edward E. K.;
Petzold, Christopher J.;
Hillson, Nathan J.;
Keasling, Jay D.

Publication type:

Article

Biochemical Characterization of β‐Amino Acid Incorporation in Fluvirucin B<sub>2</sub> Biosynthesis.

Published in:

ChemBioChem, 2018, v. 19, n. 13, p. 1391, doi. 10.1002/cbic.201800169

By:

Barajas, Jesus F.;
Zargar, Amin;
Pang, Bo;
Benites, Veronica T.;
Gin, Jennifer;
Baidoo, Edward E. K.;
Petzold, Christopher J.;
Hillson, Nathan J.;
Keasling, Jay D.

Publication type:

Article

Mechanistic Analysis of an Engineered Enzyme that Catalyzes the Formose Reaction.

Published in:

ChemBioChem, 2015, v. 16, n. 13, p. 1950, doi. 10.1002/cbic.201500228

By:

Poust, Sean;
Piety, James;
Bar‐Even, Arren;
Louw, Catherine;
Baker, David;
Keasling, Jay D.;
Siegel, Justin B.

Publication type:

Article

Whole-cell biocatalytic and de novo production of alkanes from free fatty acids in Saccharomyces cerevisiae.

Published in:

Biotechnology & Bioengineering, 2017, v. 114, n. 1, p. 232, doi. 10.1002/bit.25920

By:

Foo, Jee Loon;
Susanto, Adelia Vicanatalita;
Keasling, Jay D.;
Leong, Susanna Su Jan;
Chang, Matthew Wook

Publication type:

Article

A kinetic-based approach to understanding heterologous mevalonate pathway function in E. coli.

Published in:

Biotechnology & Bioengineering, 2015, v. 112, n. 1, p. 111, doi. 10.1002/bit.25323

By:

Weaver, Lane J.;
Sousa, Mirta M. L.;
Wang, George;
Baidoo, Edward;
Petzold, Christopher J.;
Keasling, Jay D.

Publication type:

Article

Correlation analysis of targeted proteins and metabolites to assess and engineer microbial isopentenol production.

Published in:

Biotechnology & Bioengineering, 2014, v. 111, n. 8, p. 1648, doi. 10.1002/bit.25226

By:

George, Kevin W.;
Chen, Amy;
Jain, Aakriti;
Batth, Tanveer S.;
Baidoo, Edward E.K.;
Wang, George;
Adams, Paul D.;
Petzold, Christopher J.;
Keasling, Jay D.;
Lee, Taek Soon

Publication type:

Article

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway.

Published in:

Biotechnology & Bioengineering, 2010, v. 107, n. 3, p. 421, doi. 10.1002/bit.22831

By:

Wang, Chonglong;
Yoon, Sang-Hwal;
Shah, Asad Ali;
Chung, Young-Ryun;
Kim, Jae-Yean;
Choi, Eui-Sung;
Keasling, Jay D.;
Kim, Seon-Won

Publication type:

Article

Analysis of metabolic pathways and fluxes in a newly discovered thermophilic and ethanol-tolerant Geobacillus strain.

Published in:

Biotechnology & Bioengineering, 2009, v. 102, n. 5, p. 1377, doi. 10.1002/bit.22181

By:

Tang, Yinjie J.;
Sapra, Rajat;
Joyner, Dominique;
Hazen, Terry C.;
Myers, Samuel;
Reichmuth, David;
Blanch, Harvey;
Keasling, Jay D.

Publication type:

Article

Metabolic flux analysis of Shewanella spp. reveals evolutionary robustness in central carbon metabolism.

Published in:

Biotechnology & Bioengineering, 2009, v. 102, n. 4, p. 1161, doi. 10.1002/bit.22129

By:

Tang, Yinjie J.;
Martin, Hector Garcia;
Dehal, Paramvir S.;
Deutschbauer, Adam;
Llora, Xavier;
Meadows, Adam;
Arkin, Adam;
Keasling, Jay. D.

Publication type:

Article

Redirection of flux through the FPP branch-point in Saccharomyces cerevisiae by down-regulating squalene synthase.

Published in:

Biotechnology & Bioengineering, 2008, v. 100, n. 2, p. 371, doi. 10.1002/bit.21766

By:

Paradise, Eric M.;
Kirby, James;
Chan, Rossana;
Keasling, Jay D.

Publication type:

Article

A kinetic model describing Shewanella oneidensis MR-1 growth, substrate consumption, and product secretion.

Published in:

Biotechnology & Bioengineering, 2007, v. 96, n. 1, p. 125, doi. 10.1002/bit.21101

By:

Tang, Yinjie J.;
Meadows, Adam L.;
Keasling, Jay D.

Publication type:

Article

High-level production of amorpha-4,11-diene in a two-phase partitioning bioreactor of metabolically engineered Escherichia coli.

Published in:

Biotechnology & Bioengineering, 2006, v. 95, n. 4, p. 684, doi. 10.1002/bit.21017

By:

Newman, Jack D.;
Marshall, Jessica;
Chang, Michelle;
Nowroozi, Farnaz;
Paradise, Eric;
Pitera, Douglas;
Newman, Karyn L.;
Keasling, Jay D.

Publication type:

Article

Evaluation of the effects of various culture conditions on Cr(VI) reduction by Shewanella oneidensis MR-1 in a novel high-throughput mini-bioreactor.

Published in:

Biotechnology & Bioengineering, 2006, v. 95, n. 1, p. 176, doi. 10.1002/bit.21002

By:

Tang, Yinjie J.;
Laidlaw, David;
Gani, Kishen;
Keasling, Jay D.

Publication type:

Article

Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate.

Published in:

Biotechnology & Bioengineering, 2006, v. 94, n. 6, p. 1025, doi. 10.1002/bit.20912

By:

Yoon, Sang-Hwal;
Lee, Young-Mi;
Kim, Ju-Eun;
Lee, Sook-Hee;
Lee, Joo-Hee;
Kim, Jae-Yean;
Jung, Kyung-Hwa;
Shin, Yong-Chul;
Keasling, Jay D.;
Kim, Seon-Won

Publication type:

Article

A tightly regulated inducible expression system utilizing the fim inversion recombination switch.

Published in:

Biotechnology & Bioengineering, 2006, v. 94, n. 1, p. 1, doi. 10.1002/bit.20916

By:

Ham, Timothy S.;
Lee, Sung Kuk;
Keasling, Jay D.;
Arkin, Adam P.

Publication type:

Article

Propionate-regulated high-yield protein production in Escherichia coli.

Published in:

Biotechnology & Bioengineering, 2006, v. 93, n. 5, p. 912, doi. 10.1002/bit.20784

By:

Lee, Sung Kuk;
Keasling, Jay D.

Publication type:

Article

Optimization of DsRed production in Escherichia coli: Effect of ribosome binding site sequestration on translation efficiency.

Published in:

Biotechnology & Bioengineering, 2005, v. 92, n. 5, p. 553, doi. 10.1002/bit.20630

By:

Pfleger, Brian F.;
Fawzi, Nicolas J.;
Keasling, Jay D.

Publication type:

Article

Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method.

Published in:

Biotechnology & Bioengineering, 2005, v. 91, n. 5, p. 636, doi. 10.1002/bit.20539

By:

Kang, Min Jung;
Lee, Young Mi;
Yoon, Sang Hwal;
Kim, Jung Heon;
Ock, So Won;
Jung, Kyung Hwa;
Shin, Yong Chul;
Keasling, Jay D.;
Kim, Seon Won

Publication type:

Article

Dibenzothiophene biodesulfurization pathway improvement using diagnostic GFP fusions.

Published in:

Biotechnology & Bioengineering, 2004, v. 88, n. 1, p. 94, doi. 10.1002/bit.20220

By:

Reichmuth, David S.;
Blanch, Harvey W.;
Keasling, Jay D.

Publication type:

Article

Mono and diterpene production in Escherichia coli.

Published in:

Biotechnology & Bioengineering, 2004, v. 87, n. 2, p. 200, doi. 10.1002/bit.20128

By:

Reiling, K. Kinkead;
Yoshikuni, Yasuo;
Martin, Vincent J.J.;
Newman, Jack;
Bohlmann, Jörg;
Keasling, Jay D.

Publication type:

Article

Microbioreactor arrays with parametric control for high-throughput experimentation.

Published in:

Biotechnology & Bioengineering, 2004, v. 85, n. 4, p. 376, doi. 10.1002/bit.10835

By:

Michel M. Maharbiz;
William J. Holtz;
Roger T. Howe;
Jay D. Keasling

Publication type:

Article

Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon.

Published in:

Biotechnology & Bioengineering, 2002, v. 80, n. 7, p. 762, doi. 10.1002/bit.10434

By:

Smolke, Christina D.;
Keasling, Jay D.

Publication type:

Article

Metabolic engineering of Pseudomonas putida for the utilization of parathion as a carbon and energy source.

Published in:

Biotechnology & Bioengineering, 2002, v. 78, n. 7, p. 715, doi. 10.1002/bit.10251

By:

Walker, Andy W;
Keasling, Jay D

Publication type:

Article

Effect of copy number and mRNA processing and stabilization on transcript and protein levels from an engineered dual-gene operon.

Published in:

Biotechnology & Bioengineering, 2002, v. 78, n. 4, p. 412, doi. 10.1002/bit.10218

By:

Smolke, Christina D.;
Keasling, Jay D.

Publication type:

Article

The in vivo synthesis of plant sesquiterpenes by Escherichia coli.

Published in:

Biotechnology & Bioengineering, 2001, v. 75, n. 5, p. 497, doi. 10.1002/bit.10037

By:

Martin, Vincent J.J.;
Yoshikuni, Yasuo;
Keasling, Jay D.

Publication type:

Article

Analysis of an engineered sulfate reduction pathway and cadmium precipitation on the cell surface.

Published in:

Biotechnology & Bioengineering, 2001, v. 75, n. 3, p. 285, doi. 10.1002/bit.10030

By:

Wang, Clifford L.;
Clark, Douglas S.;
Keasling, Jay D.

Publication type:

Article

Production of tranilast [ N-(3′,4′-dimethoxycinnamoyl)-anthranilic acid] and its analogs in yeast Saccharomyces cerevisiae.

Published in:

Applied Microbiology & Biotechnology, 2011, v. 89, n. 4, p. 989, doi. 10.1007/s00253-010-2939-y

By:

Eudes, Aymerick;
Baidoo, Edward E. K.;
Yang, Fan;
Burd, Helcio;
Hadi, Masood Z.;
Collins, F. William;
Keasling, Jay D.;
Loqué, Dominique

Publication type:

Article

Manipulation of intracellular magnesium levels in Saccharomyces cerevisiae with deletion of magnesium transporters.

Published in:

Applied Microbiology & Biotechnology, 2007, v. 77, n. 2, p. 411, doi. 10.1007/s00253-007-1177-4

By:

Da Costa, Bernardo M. T.;
Cornish, Katrina;
Keasling, Jay D.

Publication type:

Article

Polyphosphate kinase genes from full-scale activated sludge plants.

Published in:

Applied Microbiology & Biotechnology, 2007, v. 77, n. 1, p. 167, doi. 10.1007/s00253-007-1122-6

By:

McMahon, Katherine D.;
Yilmaz, Suzan;
He, Shaomei;
Gall, Daniel L.;
Jenkins, David;
Keasling, Jay D.

Publication type:

Article

Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis.

Published in:

Applied Microbiology & Biotechnology, 2007, v. 74, n. 1, p. 131, doi. 10.1007/s00253-006-0623-z

By:

Sang-Hwal Yoon;
Ju-Eun Kim;
Sook-Hee Lee;
Hye-Min Park;
Myung-Suk Choi;
Jae-Yean Kim;
Si-Hyoung Lee;
Yong-Chul Shin;
Keasling, Jay D.;
Seon-Won Kim

Publication type:

Article

Biosynthesis and engineering of isoprenoid small molecules.

Published in:

Applied Microbiology & Biotechnology, 2006, v. 73, n. 5, p. 980, doi. 10.1007/s00253-006-0593-1

By:

Withers, Sydnor T.;
Keasling, Jay D.

Publication type:

Article

Marker and promoter effects on heterologous expression in Aspergillus nidulans.

Published in:

Applied Microbiology & Biotechnology, 2006, v. 72, n. 5, p. 1014, doi. 10.1007/s00253-006-0368-8

By:

Lubertozzi, David;
Keasling, Jay D.

Publication type:

Article

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases.

Published in:

Journal of Industrial Microbiology & Biotechnology, 2019, v. 46, n. 8, p. 1225, doi. 10.1007/s10295-019-02189-z

By:

Barajas, Jesus F.;
McAndrew, Ryan P.;
Thompson, Mitchell G.;
Backman, Tyler W. H.;
Pang, Bo;
de Rond, Tristan;
Pereira, Jose H.;
Benites, Veronica T.;
Martín, Héctor García;
Baidoo, Edward E. K.;
Hillson, Nathan J.;
Adams, Paul D.;
Keasling, Jay D.

Publication type:

Article

Synthetic biology of polyketide synthases.

Published in:

Journal of Industrial Microbiology & Biotechnology, 2018, v. 45, n. 7, p. 621, doi. 10.1007/s10295-018-2021-9

By:

Yuzawa, Satoshi;
Backman, Tyler W. H.;
Keasling, Jay D.;
Katz, Leonard

Publication type:

Article

Expression of a synthetic Artemesia annua amorphadiene synthase in Aspergillus nidulans yields altered product distribution.

Published in:

Journal of Industrial Microbiology & Biotechnology, 2008, v. 35, n. 10, p. 1191, doi. 10.1007/s10295-008-0400-3

By:

Lubertozzi, David;
Keasling, Jay D.

Publication type:

Article

Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes.

Published in:

Nature Biotechnology, 2006, v. 24, n. 8, p. 1027, doi. 10.1038/nbt1226

By:

Pfleger, Brian F.;
Pitera, Douglas J.;
Smolke, Christina D.;
Keasling, Jay D.

Publication type:

Article

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.

Published in:

Nature Biotechnology, 2003, v. 21, n. 7, p. 796, doi. 10.1038/nbt833

By:

Martin, Vincent J J;
Pitera, Douglas J;
Withers, Sydnor T;
Newman, Jack D;
Keasling, Jay D

Publication type:

Article

Encoding substrates with mass tags to resolve stereospecific reactions using Nimzyme.

Published in:

Rapid Communications in Mass Spectrometry: RCM, 2012, v. 26, n. 6, p. 611, doi. 10.1002/rcm.6134

By:

Deng, Kai;
George, Kevin W.;
Reindl, Wolfgang;
Keasling, Jay D.;
Adams, Paul D.;
Lee, Taek Soon;
Singh, Anup K.;
Northen, Trent R.

Publication type:

Article

Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for α‐Diazoester Production.

Published in:

Angewandte Chemie, 2023, v. 135, n. 28, p. 1, doi. 10.1002/ange.202304646

By:

Van Cura, Devon;
Ng, Tai L.;
Huang, Jing;
Hager, Harry;
Hartwig, John F.;
Keasling, Jay D.;
Balskus, Emily P.

Publication type:

Article

A High‐Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts.

Published in:

Angewandte Chemie, 2019, v. 131, n. 30, p. 10220, doi. 10.1002/ange.201901782

By:

de Rond, Tristan;
Gao, Jian;
Zargar, Amin;
de Raad, Markus;
Cunha, Jack;
Northen, Trent R.;
Keasling, Jay D.

Publication type:

Article

Optimizing the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies.

Published in:

Biotechnology & Bioengineering, 2021, v. 118, n. 1, p. 279, doi. 10.1002/bit.27569

By:

Walls, Laura E.;
Malcı, Koray;
Nowrouzi, Behnaz;
Li, Rachel A.;
d'Espaux, Leo;
Wong, Jeff;
Dennis, Jonathan A.;
Semião, Andrea J. C.;
Wallace, Stephen;
Martinez, José L.;
Keasling, Jay D.;
Rios‐Solis, Leonardo

Publication type:

Article

Improving methyl ketone production in <italic>Escherichia coli</italic> by heterologous expression of NADH‐dependent FabG.

Published in:

Biotechnology & Bioengineering, 2018, v. 115, n. 5, p. 1161, doi. 10.1002/bit.26558

By:

Goh, Ee‐Been;
Chen, Yan;
Petzold, Christopher J.;
Keasling, Jay D.;
Beller, Harry R.

Publication type:

Article

Toward industrial production of isoprenoids in <italic>Escherichia coli</italic>: Lessons learned from CRISPR‐Cas9 based optimization of a chromosomally integrated mevalonate pathway.

Published in:

Biotechnology & Bioengineering, 2018, v. 115, n. 4, p. 1000, doi. 10.1002/bit.26530

By:

Alonso‐Gutierrez, Jorge;
Koma, Daisuke;
Hu, Qijun;
Yang, Yuchen;
Chan, Leanne J. G.;
Petzold, Christopher J.;
Adams, Paul D.;
Vickers, Claudia E.;
Nielsen, Lars K.;
Keasling, Jay D.;
Lee, Taek S.

Publication type:

Article

Production of jet fuel precursor monoterpenoids from engineered Escherichia coli.

Published in:

Biotechnology & Bioengineering, 2017, v. 114, n. 8, p. 1703, doi. 10.1002/bit.26296

By:

Mendez‐Perez, Daniel;
Alonso‐Gutierrez, Jorge;
Hu, Qijun;
Molinas, Margaux;
Baidoo, Edward E.K.;
Wang, George;
Chan, Leanne J.G.;
Adams, Paul D.;
Petzold, Christopher J.;
Keasling, Jay D.;
Lee, Taek S.

Publication type:

Article

Cyanobacterial carbon metabolism: Fluxome plasticity and oxygen dependence.

Published in:

Biotechnology & Bioengineering, 2017, v. 114, n. 7, p. 1593, doi. 10.1002/bit.26287

By:

Wan, Ni;
DeLorenzo, Drew M.;
He, Lian;
You, Le;
Immethun, Cheryl M.;
Wang, George;
Baidoo, Edward E. K.;
Hollinshead, Whitney;
Keasling, Jay D.;
Moon, Tae Seok;
Tang, Yinjie J.

Publication type:

Article

Designed divergent evolution of enzyme function.

Published in:

2006

By:

Yoshikuni, Yasuo;
Ferrin, Thomas E.;
Keasling, Jay D.

Publication type:

Letter