Works by Hussack, Greg

Results: 34

Targeting surface-layer proteins with single-domain antibodies: a potential therapeutic approach against Clostridium difficile-associated disease.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 20, p. 8549, doi. 10.1007/s00253-015-6594-1

By:

Kandalaft, Hiba;
Hussack, Greg;
Aubry, Annie;
Faassen, Henk;
Guan, Yonghong;
Arbabi-Ghahroudi, Mehdi;
MacKenzie, Roger;
Logan, Susan;
Tanha, Jamshid

Publication type:

Article

In vivo brain delivery of BBB-enabled iduronate 2-sulfatase in rats.

Published in:

Fluids & Barriers of the CNS, 2025, v. 22, n. 1, p. 1, doi. 10.1186/s12987-024-00617-6

By:

Costain, Will J.;
Haqqani, Arsalan S.;
Hussack, Greg;
van Faassen, Henk;
Lessard, Etienne;
Ling, Binbing;
Brunette, Eric;
Ly, Dao;
Fang, Hung;
Bultinck, Jennyfer;
Geysens, Steven;
Pynaert, Gwenda;
Piens, Kathleen;
Ryckaert, Stefan;
Fudalej, Franck;
Vervecken, Wouter;
Stanimirovic, Danica

Publication type:

Article

An update on antibody-based immunotherapies for Clostridium difficile infection.

Published in:

Clinical & Experimental Gastroenterology, 2016, v. 9, p. 209, doi. 10.2147/CEG.S84017

By:

Hussack, Greg;
Tanha, Jamshid

Publication type:

Article

Structure of a Therapeutic Antibody in Complex with MUC16 Reveals a Conformational Epitope Influenced by Antigen Glycosylation.

Published in:

FASEB Journal, 2022, v. 36, p. N.PAG, doi. 10.1096/fasebj.2022.36.S1.R5166

By:

Brooks, Cory L.;
Aguilar, Eric N.;
White, Brandy;
van Faassen, Henk;
Michaud, Sarah;
Goodlett, David;
Brooks, Teresa;
Hussack, Greg;
Henry, Kevin;
Radhakrishnan, Prakash

Publication type:

Article

Targeting insulin‐like growth factor‐1 receptor (IGF1R) for brain delivery of biologics.

Published in:

FASEB Journal, 2022, v. 36, n. 3, p. 1, doi. 10.1096/fj.202101644R

By:

Alata, Wael;
Yogi, Alvaro;
Brunette, Eric;
Delaney, Christie E.;
van Faassen, Henk;
Hussack, Greg;
Iqbal, Umar;
Kemmerich, Kristin;
Haqqani, Arsalan S.;
Moreno, Maria J.;
Stanimirovic, Danica B.

Publication type:

Article

Structure of the Fab fragment of a humanized 5E5 antibody to a cancer‐specific Tn‐MUC1 epitope.

Published in:

Acta Crystallographica: Section D, Structural Biology, 2025, v. 81, n. 5, p. 223, doi. 10.1107/S2059798325002554

By:

Li, Wei;
Mandel, Ulla;
van Faassen, Henk;
Parker, Matthew J.;
Legg, Max S. G.;
Hussack, Greg;
Clausen, Henrik;
Evans, Stephen V.

Publication type:

Article

Brain Delivery of IGF1R5, a Single-Domain Antibody Targeting Insulin-like Growth Factor-1 Receptor.

Published in:

Pharmaceutics, 2022, v. 14, n. 7, p. N.PAG, doi. 10.3390/pharmaceutics14071452

By:

Yogi, Alvaro;
Hussack, Greg;
van Faassen, Henk;
Haqqani, Arsalan S.;
Delaney, Christie E.;
Brunette, Eric;
Sandhu, Jagdeep K.;
Hewitt, Melissa;
Sulea, Traian;
Kemmerich, Kristin;
Stanimirovic, Danica B.

Publication type:

Article

Modulating antibody‐dependent cellular cytotoxicity of epidermal growth factor receptor‐specific heavy‐chain antibodies through hinge engineering.

Published in:

Immunology & Cell Biology, 2019, v. 97, n. 6, p. 526, doi. 10.1111/imcb.12238

By:

D'Eall, Calvin;
Pon, Robert A;
Rossotti, Martin A;
Krahn, Natalie;
Spearman, Maureen;
Callaghan, Deborah;
Faassen, Henk;
Hussack, Greg;
Stetefeld, Jörg;
Butler, Michael;
Durocher, Yves;
Zhang, Jianbing;
Henry, Kevin A;
Tanha, Jamshid

Publication type:

Article

Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human VH/VL Single-Domain Antibodies from In Vitro Display Libraries.

Published in:

Frontiers in Immunology, 2017, p. 1, doi. 10.3389/fimmu.2017.01759

By:

Henry, Kevin A.;
Dae Young Kim;
Kandalaft, Hiba;
Lowden, Michael J.;
Qingling Yang;
Schrag, Joseph D.;
Hussack, Greg;
MacKenzie, C. Roger;
Tanha, Jamshid

Publication type:

Article

A Novel Affinity Tag, ABTAG, and Its Application to the Affinity Screening of Single-Domain Antibodies Selected by Phage Display.

Published in:

Frontiers in Immunology, 2017, p. 1, doi. 10.3389/fimmu.2017.01406

By:

Hussack, Greg;
Baral, Toya Nath;
Baardsnes, Jason;
van Faassen, Henk;
Raphael, Shalini;
Henry, Kevin A.;
Jianbing Zhang;
MacKenzie, C. Roger

Publication type:

Article

Isolation and characterization of camelid single-domain antibodies against HER2.

Published in:

BMC Research Notes, 2018, v. 11, n. 1, p. N.PAG, doi. 10.1186/s13104-018-3955-8

By:

Hussack, Greg;
Raphael, Shalini;
Lowden, Michael J.;
Henry, Kevin A.

Publication type:

Article

A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies.

Published in:

PLoS ONE, 2016, v. 11, n. 9, p. 1, doi. 10.1371/journal.pone.0163113

By:

Henry, Kevin A.;
Sulea, Traian;
van Faassen, Henk;
Hussack, Greg;
Purisima, Enrico O.;
MacKenzie, C. Roger;
Arbabi-Ghahroudi, Mehdi

Publication type:

Article

Single-Domain Antibodies Represent Novel Alternatives to Monoclonal Antibodies as Targeting Agents against the Human Papillomavirus 16 E6 Protein.

Published in:

International Journal of Molecular Sciences, 2019, v. 20, n. 9, p. 2088, doi. 10.3390/ijms20092088

By:

Togtema, Melissa;
Hussack, Greg;
Dayer, Guillem;
Teghtmeyer, Megan R.;
Raphael, Shalini;
Tanha, Jamshid;
Zehbe, Ingeborg

Publication type:

Article

Arsenal of nanobodies shows broad-spectrum neutralization against SARS-CoV-2 variants of concern in vitro and in vivo in hamster models.

Published in:

Communications Biology, 2022, v. 5, n. 1, p. 1, doi. 10.1038/s42003-022-03866-z

By:

Rossotti, Martin A.;
van Faassen, Henk;
Tran, Anh T.;
Sheff, Joey;
Sandhu, Jagdeep K.;
Duque, Diana;
Hewitt, Melissa;
Wen, Xiaoxue;
Bavananthasivam, Jegarubee;
Beitari, Saina;
Matte, Kevin;
Laroche, Geneviève;
Giguère, Patrick M.;
Gervais, Christian;
Stuible, Matthew;
Guimond, Julie;
Perret, Sylvie;
Hussack, Greg;
Langlois, Marc-André;
Durocher, Yves

Publication type:

Article

Evaluation of a noncanonical Cys40‐Cys55 disulfide linkage for stabilization of single‐domain antibodies.

Published in:

Protein Science: A Publication of the Protein Society, 2019, v. 28, n. 5, p. 881, doi. 10.1002/pro.3595

By:

Kim, Dae Young;
Kandalaft, Hiba;
Hussack, Greg;
Raphael, Shalini;
Ding, Wen;
Kelly, John F.;
Henry, Kevin A.;
Tanha, Jamshid

Publication type:

Article

Epitope mapping of a blood–brain barrier crossing antibody targeting the cysteine-rich region of IGF1R using hydrogen-exchange mass spectrometry enabled by electrochemical reduction.

Published in:

Journal of Biochemistry, 2023, v. 173, n. 2, p. 95, doi. 10.1093/jb/mvac088

By:

Sheff, Joey;
Kelly, John;
Foss, Mary;
Brunette, Eric;
Kemmerich, Kristin;
Faassen, Henk van;
Raphael, Shalini;
Hussack, Greg;
Comamala, Gerard;
Rand, Kasper;
Stanimirovic, Danica B.

Publication type:

Article

Editorial: Single-domain antibodies—biology, engineering and emerging applications, volume II.

Published in:

Frontiers in Immunology, 2024, p. 1, doi. 10.3389/fimmu.2024.1537054

By:

Henry, Kevin A.;
Hussack, Greg;
Gettemans, Jan;
Brooks, Cory L.

Publication type:

Article

Neutralization of Clostridium difficile toxin B with VHH-Fc fusions targeting the delivery and CROPs domains.

Published in:

PLoS ONE, 2018, v. 13, n. 12, p. 1, doi. 10.1371/journal.pone.0208978

By:

Hussack, Greg;
Ryan, Shannon;
van Faassen, Henk;
Rossotti, Martin;
MacKenzie, C. Roger;
Tanha, Jamshid

Publication type:

Article

Role of the non‐hypervariable FR3 D‐E loop in single‐domain antibody recognition of haptens and carbohydrates.

Published in:

Journal of Molecular Recognition, 2019, v. 32, n. 11, p. 1, doi. 10.1002/jmr.2805

By:

Henry, Kevin A.;
Hussack, Greg;
Kumaran, Jyothi;
Gilbert, Michel;
MacKenzie, C. Roger;
Sulea, Traian;
Arbabi‐Ghahroudi, Mehdi

Publication type:

Article

Serum albumin‐binding VHHs with variable pH sensitivities enable tailored half‐life extension of biologics.

Published in:

FASEB Journal, 2020, v. 34, n. 6, p. 8155, doi. 10.1096/fj.201903231R

By:

van Faassen, Henk;
Ryan, Shannon;
Henry, Kevin A.;
Raphael, Shalini;
Yang, Qingling;
Rossotti, Martin A.;
Brunette, Eric;
Jiang, Susan;
Haqqani, Arsalan S.;
Sulea, Traian;
MacKenzie, C. Roger;
Tanha, Jamshid;
Hussack, Greg

Publication type:

Article

Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.

Published in:

Applied Microbiology & Biotechnology, 2024, v. 108, n. 1, p. 1, doi. 10.1007/s00253-024-13033-5

By:

Lei, Eric K.;
Azmat, Aruba;
Henry, Kevin A.;
Hussack, Greg

Publication type:

Article

Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.

Published in:

Applied Microbiology & Biotechnology, 2024, v. 108, n. 1, p. 1, doi. 10.1007/s00253-024-13033-5

By:

Lei, Eric K.;
Azmat, Aruba;
Henry, Kevin A.;
Hussack, Greg

Publication type:

Article

Isolation and characterization of a VHH targeting the Acinetobacter baumannii cell surface protein CsuA/B.

Published in:

Applied Microbiology & Biotechnology, 2023, v. 107, n. 14, p. 4567, doi. 10.1007/s00253-023-12594-1

By:

Lei, Eric K.;
Ryan, Shannon;
van Faassen, Henk;
Foss, Mary;
Robotham, Anna;
Baltat, Isabel;
Fulton, Kelly;
Henry, Kevin A.;
Chen, Wangxue;
Hussack, Greg

Publication type:

Article

Camelid single-domain antibodies raised by DNA immunization are potent inhibitors of EGFR signaling.

Published in:

Biochemical Journal, 2019, v. 476, n. 1, p. 39, doi. 10.1042/BCJ20180795

By:

Rossotti, Martin A.;
Henry, Kevin A.;
van Faassen, Henk;
Tanha, Jamshid;
Callaghan, Deborah;
Hussack, Greg;
Arbabi-Ghahroudi, Mehdi;
MacKenzie, C. Roger

Publication type:

Article

A Novel Antigen Design Strategy to Isolate Single‐Domain Antibodies that Target Human Nav1.7 and Reduce Pain in Animal Models.

Published in:

Advanced Science, 2024, v. 11, n. 40, p. 1, doi. 10.1002/advs.202405432

By:

Martina, Marzia;
Banderali, Umberto;
Yogi, Alvaro;
Arbabi Ghahroudi, Mehdi;
Liu, Hong;
Sulea, Traian;
Durocher, Yves;
Hussack, Greg;
van Faassen, Henk;
Chakravarty, Balu;
Liu, Qing Yan;
Iqbal, Umar;
Ling, Binbing;
Lessard, Etienne;
Sheff, Joey;
Robotham, Anna;
Callaghan, Debbie;
Moreno, Maria;
Comas, Tanya;
Ly, Dao

Publication type:

Article

Defining the epitope of a blood–brain barrier crossing single domain antibody specific for the type 1 insulin-like growth factor receptor.

Published in:

Scientific Reports, 2021, v. 11, n. 1, p. 1, doi. 10.1038/s41598-021-83198-w

By:

Sheff, Joey;
Wang, Ping;
Xu, Ping;
Arbour, Melanie;
Masson, Luke;
van Faassen, Henk;
Hussack, Greg;
Kemmerich, Kristin;
Brunette, Eric;
Stanimirovic, Danica;
Hill, Jennifer J.;
Kelly, John;
Ni, Feng

Publication type:

Article

Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability.

Published in:

PLoS ONE, 2011, v. 6, n. 11, p. 1, doi. 10.1371/journal.pone.0028218

By:

Hussack, Greg;
Hirama, Tomoko;
Ding, Wen;
MacKenzie, Roger;
Tanha, Jamshid

Publication type:

Article

Multivalent Anchoring and Oriented Display of Single-Domain Antibodies on Cellulose.

Published in:

Sensors (14248220), 2009, v. 9, n. 7, p. 5351, doi. 10.3390/s90705351

By:

Hussack, Greg;
Yan Luo;
Veldhuis, Linda;
Hall, J. Christopher;
Tanha, Jamshid;
MacKenzie, Roger

Publication type:

Article

Structure-guided design of a potent Clostridioides difficile toxin A inhibitor.

Published in:

Frontiers in Microbiology, 2023, v. 14, p. 1, doi. 10.3389/fmicb.2023.1110541

By:

Hussack, Greg;
Rossotti, Martin A.;
van Faassen, Henk;
Tomohiko Murase;
Eugenio, Luiz;
Schrag, Joseph D.;
Ng, Kenneth K.-S.;
Tanha, Jamshid

Publication type:

Article

Isolation of TGF-ß-neutralizing single-domain antibodies of predetermined epitope specificity using next-generation DNA sequencing.

Published in:

PEDS: Protein Engineering, Design & Selection, 2016, v. 29, n. 10, p. 439, doi. 10.1093/protein/gzw043

By:

Henry, Kevin A.;
Hussack, Greg;
Collins, Cathy;
Zwaagstra, John C.;
Tanha, Jamshid;
MacKenzie, C. Roger

Publication type:

Article

Identification of cross-reactive single-domain antibodies against serum albumin using next-generation DNA sequencing.

Published in:

PEDS: Protein Engineering, Design & Selection, 2015, v. 28, n. 10, p. 379, doi. 10.1093/protein/gzv039

By:

Henry, Kevin A.;
Tanha, Jamshid;
Hussack, Greg

Publication type:

Article

Protease-resistant single-domain antibodies inhibit Campylobacter jejuni motility.

Published in:

PEDS: Protein Engineering, Design & Selection, 2014, v. 27, n. 6, p. 191, doi. 10.1093/protein/gzu011

By:

Hussack, Greg;
Riazi, Ali;
Ryan, Shannon;
van Faassen, Henk;
MacKenzie, Roger;
Tanha, Jamshid;
Arbabi-Ghahroudi, Mehdi

Publication type:

Article

A VL single-domain antibody library shows a high-propensity to yield non-aggregating binders †.

Published in:

PEDS: Protein Engineering, Design & Selection, 2012, v. 25, n. 6, p. 313, doi. 10.1093/protein/gzs014

By:

Hussack, Greg;
Keklikian, Artine;
Alsughayyir, Jawaher;
Hanifi-Moghaddam, Pejman;
Arbabi-Ghahroudi, Mehdi;
van Faassen, Henk;
Hou, Sheng T.;
Sad, Subash;
MacKenzie, Roger;
Tanha, Jamshid

Publication type:

Article

Optimization of synthetic human VH affinity and solubility through in vitro affinity maturation and minimal camelization.

Published in:

Protein Science: A Publication of the Protein Society, 2025, v. 34, n. 5, p. 1, doi. 10.1002/pro.70114

By:

Bélanger, Kasandra;
Wu, Cunle;
Sulea, Traian;
van Faassen, Henk;
Callaghan, Deborah;
Aubry, Annie;
Sasseville, Marc;
Hussack, Greg;
Tanha, Jamshid

Publication type:

Article

Works by Hussack, Greg

Targeting surface-layer proteins with single-domain antibodies: a potential therapeutic approach against Clostridium difficile-associated disease.

In vivo brain delivery of BBB-enabled iduronate 2-sulfatase in rats.

An update on antibody-based immunotherapies for Clostridium difficile infection.

Structure of a Therapeutic Antibody in Complex with MUC16 Reveals a Conformational Epitope Influenced by Antigen Glycosylation.

Targeting insulin‐like growth factor‐1 receptor (IGF1R) for brain delivery of biologics.

Structure of the Fab fragment of a humanized 5E5 antibody to a cancer‐specific Tn‐MUC1 epitope.

Brain Delivery of IGF1R5, a Single-Domain Antibody Targeting Insulin-like Growth Factor-1 Receptor.

Modulating antibody‐dependent cellular cytotoxicity of epidermal growth factor receptor‐specific heavy‐chain antibodies through hinge engineering.

Stability-Diversity Tradeoffs Impose Fundamental Constraints on Selection of Synthetic Human V<sub>H</sub>/V<sub>L</sub> Single-Domain Antibodies from In Vitro Display Libraries.

A Novel Affinity Tag, ABTAG, and Its Application to the Affinity Screening of Single-Domain Antibodies Selected by Phage Display.

Isolation and characterization of camelid single-domain antibodies against HER2.

A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies.

Single-Domain Antibodies Represent Novel Alternatives to Monoclonal Antibodies as Targeting Agents against the Human Papillomavirus 16 E6 Protein.

Arsenal of nanobodies shows broad-spectrum neutralization against SARS-CoV-2 variants of concern in vitro and in vivo in hamster models.

Evaluation of a noncanonical Cys40‐Cys55 disulfide linkage for stabilization of single‐domain antibodies.

Epitope mapping of a blood–brain barrier crossing antibody targeting the cysteine-rich region of IGF1R using hydrogen-exchange mass spectrometry enabled by electrochemical reduction.

Editorial: Single-domain antibodies—biology, engineering and emerging applications, volume II.

Neutralization of Clostridium difficile toxin B with V<sub>H</sub>H-Fc fusions targeting the delivery and CROPs domains.

Role of the non‐hypervariable FR3 D‐E loop in single‐domain antibody recognition of haptens and carbohydrates.

Serum albumin‐binding V<sub>H</sub>Hs with variable pH sensitivities enable tailored half‐life extension of biologics.

Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.

Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.

Isolation and characterization of a VHH targeting the Acinetobacter baumannii cell surface protein CsuA/B.

Camelid single-domain antibodies raised by DNA immunization are potent inhibitors of EGFR signaling.

A Novel Antigen Design Strategy to Isolate Single‐Domain Antibodies that Target Human Nav1.7 and Reduce Pain in Animal Models.

Defining the epitope of a blood–brain barrier crossing single domain antibody specific for the type 1 insulin-like growth factor receptor.

Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability.

Multivalent Anchoring and Oriented Display of Single-Domain Antibodies on Cellulose.

Structure-guided design of a potent Clostridioides difficile toxin A inhibitor.

Isolation of TGF-ß-neutralizing single-domain antibodies of predetermined epitope specificity using next-generation DNA sequencing.

Identification of cross-reactive single-domain antibodies against serum albumin using next-generation DNA sequencing.

Protease-resistant single-domain antibodies inhibit Campylobacter jejuni motility.

A VL single-domain antibody library shows a high-propensity to yield non-aggregating binders †.

Optimization of synthetic human V<sub>H</sub> affinity and solubility through in vitro affinity maturation and minimal camelization.