Works by Huggett, Jim F

Results: 40

Low unspliced cell-associated HIV RNA in early treated adolescents living with HIV on long suppressive ART.

Published in:

Frontiers in Immunology, 2024, p. 1, doi. 10.3389/fimmu.2024.1334236

By:

Gärtner, Kathleen;
Domínguez-Rodríguez, Sara;
Heaney, Judith;
Gkouleli, Triantafylia;
Grant, Paul;
Dorgham, Karim;
Sauce, Delphine;
Soulie, Cathia;
Busby, Eloise J.;
O'Sullivan, Denise M.;
Spyer, Moira;
Botha, Johannes C.;
Angeles Muñoz-Fernandez, Maria;
Tagarro, Alfredo;
Cotugno, Nicola;
Huggett, Jim F.;
Klein, Nigel;
Palma, Paolo;
Rojo Conejo, Pablo;
Foster, Caroline

Publication type:

Article

Monkeypox: another test for PCR.

Published in:

Eurosurveillance (15607917), 2022, v. 27, n. 32, p. 19, doi. 10.2807/1560-7917.ES.2022.27.32.2200497

By:

Huggett, Jim F.;
French, David;
O’Sullivan, Denise M.;
Moran-Gilad, Jacob;
Zumla, Alimuddin

Publication type:

Article

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.

Published in:

Nucleic Acids Research, 2012, v. 40, n. 11, p. e82, doi. 10.1093/nar/gks203

By:

Whale, Alexandra S.;
Huggett, Jim F.;
Cowen, Simon;
Speirs, Valerie;
Shaw, Jacqui;
Ellison, Stephen;
Foy, Carole A.;
Scott, Daniel J.

Publication type:

Article

Behind the scenes of EQA – characteristics, capabilities, benefits and assets of external quality assessment (EQA): Part V – Benefits for stakeholders other than participants.

Published in:

Clinical Chemistry & Laboratory Medicine, 2025, v. 63, n. 5, p. 898, doi. 10.1515/cclm-2024-1293

By:

Buchta, Christoph;
De la Salle, Barbara;
Marrington, Rachel;
Aburto Almonacid, Andrés;
Albarède, Stéphanie;
Badrick, Tony;
Bullock, David;
Cobbaert, Christa M.;
Coucke, Wim;
Delatour, Vincent;
Faria, Ana Paula;
Geilenkeuser, Wolf-Jochen;
Griesmacher, Andrea;
Huggett, Jim F.;
Ianovska, Viktoriia;
Kammel, Martin;
Kessler, Anja;
Körmöczi, Günther F.;
Meijer, Piet;
Miranda, Armandina

Publication type:

Article

Behind the scenes of EQA–characteristics, capabilities, benefits and assets of external quality assessment (EQA): Part IV – Benefits for participant laboratories.

Published in:

Clinical Chemistry & Laboratory Medicine, 2025, v. 63, n. 5, p. 879, doi. 10.1515/cclm-2024-1292

By:

Buchta, Christoph;
De la Salle, Barbara;
Marrington, Rachel;
Albarède, Stéphanie;
Badrick, Tony;
Bicker, Wolfgang;
Bietenbeck, Andreas;
Bullock, David;
Delatour, Vincent;
Dequeker, Elisabeth;
Flasch, Mira;
Geilenkeuser, Wolf-Jochen;
van Hellemond, Jaap;
Huggett, Jim F.;
Ianovska, Viktoriia;
Kaiser, Patricia;
Kammel, Martin;
Kessler, Anja;
Laudus, Nele;
Luppa, Peter B.

Publication type:

Article

Behind the scenes of EQA – characteristics, capabilities, benefits and assets of external quality assessment (EQA): Part III – EQA samples.

Published in:

Clinical Chemistry & Laboratory Medicine, 2025, v. 63, n. 5, p. 868, doi. 10.1515/cclm-2024-1291

By:

Buchta, Christoph;
Marrington, Rachel;
De la Salle, Barbara;
Albarède, Stéphanie;
Albe, Xavier;
Badrick, Tony;
Berghäll, Heidi;
Bullock, David;
Cobbaert, Christa M.;
Coucke, Wim;
Delatour, Vincent;
Geilenkeuser, Wolf-Jochen;
Griesmacher, Andrea;
Henriksen, Gitte M.;
Huggett, Jim F.;
Juhos, István;
Kammel, Martin;
Luppa, Peter B.;
Meijer, Piet;
Pelanti, Jonna

Publication type:

Article

Behind the scenes of EQA – characteristics, capabilities, benefits and assets of external quality assessment (EQA): Part II – EQA cycles.

Published in:

Clinical Chemistry & Laboratory Medicine, 2025, v. 63, n. 5, p. 859, doi. 10.1515/cclm-2024-1290

By:

Buchta, Christoph;
Marrington, Rachel;
De la Salle, Barbara;
Albarède, Stéphanie;
Badrick, Tony;
Berghäll, Heidi;
Bullock, David;
Coucke, Wim;
Delatour, Vincent;
Geilenkeuser, Wolf-Jochen;
Griesmacher, Andrea;
Henriksen, Gitte M.;
Huggett, Jim F.;
Luppa, Peter B.;
Pelanti, Jonna;
Pezzati, Paola;
Sandberg, Sverre;
Spannagl, Michael;
Thelen, Marc;
Restelli, Veronica

Publication type:

Article

Behind the scenes of EQA – characteristics, capabilities, benefits and assets of external quality assessment (EQA): Part I – EQA in general and EQA programs in particular.

Published in:

Clinical Chemistry & Laboratory Medicine, 2025, v. 63, n. 5, p. 844, doi. 10.1515/cclm-2024-1289

By:

Buchta, Christoph;
Marrington, Rachel;
De la Salle, Barbara;
Albarède, Stéphanie;
Badrick, Tony;
Bietenbeck, Andreas;
Bullock, David;
Cadamuro, Janne;
Delatour, Vincent;
Dusinovic, Enes;
Geilenkeuser, Wolf-Jochen;
Gidske, Gro;
Griesmacher, Andrea;
Haliassos, Alexander;
Holzhauser, Derek;
Huggett, Jim F.;
Karathanos, Serafeim;
Pezzati, Paola;
Sandberg, Sverre;
Sarkar, Aditi

Publication type:

Article

Different screening strategies (single or dual) for the diagnosis of suspected latent tuberculosis: a cost effectiveness analysis.

Published in:

BMC Pulmonary Medicine, 2010, v. 10, p. 7, doi. 10.1186/1471-2466-10-7

By:

Pooran, Anil;
Booth, Helen;
Miller, Robert F.;
Scott, Geoff;
Badri, Motasim;
Huggett, Jim F.;
Rook, Graham;
Zumla, Alimuddin;
Dheda, Keertan

Publication type:

Article

RT-qPCR Diagnostics: The "Drosten" SARS-CoV-2 Assay Paradigm.

Published in:

International Journal of Molecular Sciences, 2021, v. 22, n. 16, p. 8702, doi. 10.3390/ijms22168702

By:

Bustin, Stephen;
Kirvell, Sara;
Huggett, Jim F.;
Nolan, Tania

Publication type:

Article

Assessing the Accuracy of Quantitative Molecular Microbial Profiling.

Published in:

International Journal of Molecular Sciences, 2014, v. 15, n. 11, p. 21476, doi. 10.3390/ijms151121476

By:

O'Sullivan, Denise M.;
Laver, Thomas;
Temisak, Sasithon;
Redshaw, Nicholas;
Harris, Kathryn A.;
Foy, Carole A.;
Studholme, David J.;
Huggett, Jim F.

Publication type:

Article

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA.

Published in:

PLoS ONE, 2018, v. 13, n. 3, p. 1, doi. 10.1371/journal.pone.0194630

By:

Gale, Davina;
Lawson, Andrew R. J.;
Howarth, Karen;
Madi, Mikidache;
Durham, Bradley;
Smalley, Sarah;
Calaway, John;
Blais, Shannon;
Jones, Greg;
Clark, James;
Dimitrov, Peter;
Pugh, Michelle;
Woodhouse, Samuel;
Epstein, Michael;
Fernandez-Gonzalez, Ana;
Whale, Alexandra S.;
Huggett, Jim F.;
Foy, Carole A.;
Jones, Gerwyn M.;
Raveh-Amit, Hadas

Publication type:

Article

In Vivo and In Vitro Studies of a Novel Cytokine, Interleukin 4δ2, in Pulmonary Tuberculosis.

Published in:

American Journal of Respiratory & Critical Care Medicine, 2005, v. 172, n. 4, p. 501, doi. 10.1164/rccm.200502-278OC

By:

Dheda, Keertan;
Jung-Su Chang;
Breen, Ronan A. M.;
Kim, Louise U.;
Haddock, Jamanda A.;
Huggett, Jim F.;
Johnson, Margaret A.;
Rook, Graham A. W.;
Zumla, Alimuddin

Publication type:

Article

Type 2 Cytokines in Respiratory Syncytial Virus Bronchiolitis.

Published in:

American Journal of Respiratory & Critical Care Medicine, 2004, v. 169, n. 10, p. 1167, doi. 10.1164/ajrccm.169.10.958

By:

DHEDA, KEERTAN;
HUGGETT, JIM F.;
KIM, LOUISE U.;
ZUMLA, ALIMUDDIN;
LEGG, JULIAN P.;
HUSSAIN, IMRAN R.;
WARNER, JILL A.;
JOHNSTON, SEBASTIAN L.;
WARNER, JOHN O.

Publication type:

Article

Standards and Metrology for Viral Vectors as Molecular Tools: Outcomes from a CCQM Workshop.

Published in:

Biologics, 2024, v. 4, n. 2, p. 187, doi. 10.3390/biologics4020013

By:

Campbell, Jonathan J.;
Almond, Neil;
Bae, Young-Kyong;
Bhuller, Ravneet;
Briones, Andrea;
Cho, Sang-Joon;
Cleveland, Megan H.;
Cleveland IV, Thomas E.;
Galaway, Francis;
He, Hua-Jun;
Herbrand, Ulrike;
Huggett, Jim F.;
Kempster, Sarah;
Kepiro, Ibolya E.;
Khan, Arifa S.;
Kwee, Edward;
Li, Wilson;
Lin-Gibson, Sheng;
Luckau, Luise;
Minelli, Caterina

Publication type:

Article

Evaluation of image analysis tools for the measurement of cellular morphology.

Published in:

Frontiers in Cell & Developmental Biology, 2025, p. 1, doi. 10.3389/fcell.2025.1572212

By:

Bourn, Matthew D.;
Daly, Lauren F.;
Huggett, Jim F.;
Braybrook, Julian;
Rivera, Jeanne F.

Publication type:

Article

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis.

Published in:

BMC Genomics, 2014, v. 15, n. 1, p. 3394, doi. 10.1186/1471-2164-15-1174

By:

Redshaw, Nicholas;
Huggett, Jim F.;
Taylor, Martin S.;
Foy, Carole A.;
Devonshire, Alison S.

Publication type:

Article

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs.

Published in:

Genome Medicine, 2019, v. 11, n. 1, p. N.PAG, doi. 10.1186/s13073-019-0650-x

By:

Phelan, Jody E.;
O'Sullivan, Denise M.;
Machado, Diana;
Ramos, Jorge;
Oppong, Yaa E. A.;
Campino, Susana;
O'Grady, Justin;
McNerney, Ruth;
Hibberd, Martin L.;
Viveiros, Miguel;
Huggett, Jim F.;
Clark, Taane G.

Publication type:

Article

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines Free.

Published in:

Clinical Chemistry, 2025, v. 71, n. 6, p. 634, doi. 10.1093/clinchem/hvaf043

By:

Bustin, Stephen A;
Ruijter, Jan M;
Hoff, Maurice J B van den;
Kubista, Mikael;
Pfaffl, Michael W;
Shipley, Gregory L;
Tran, Nham;
Rödiger, Stefan;
Untergasser, Andreas;
Mueller, Reinhold;
Nolan, Tania;
Milavec, Mojca;
Burns, Malcolm J;
Huggett, Jim F;
Vandesompele, Jo;
Wittwer, Carl T

Publication type:

Article

The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing.

Published in:

Clinical Chemistry, 2025, v. 71, n. 3, p. 378, doi. 10.1093/clinchem/hvae187

By:

Falak, Samreen;
O'Sullivan, Denise M;
Cleveland, Megan H;
Cowen, Simon;
Busby, Eloise J;
Devonshire, Alison S;
Valiente, Esmeralda;
Jones, Gerwyn M;
Kammel, Martin;
Milavec, Mojca;
Vierbaum, Laura;
Schellenberg, Ingo;
Zeichhardt, Heinz;
Kummrow, Andreas;
Vallone, Peter M;
Macdonald, Rainer;
Huggett, Jim F

Publication type:

Article

The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19.

Published in:

Clinical Chemistry, 2022, v. 68, n. 1, p. 153, doi. 10.1093/clinchem/hvab219

By:

Evans, Daniel;
Cowen, Simon;
Kammel, Martin;
O'Sullivan, Denise M.;
Stewart, Graham;
Grunert, Hans-Peter;
Moran-Gilad, Jacob;
Verwilt, Jasper;
Jiwon In;
Vandesompele, Jo;
Harris, Kathryn;
Ki Ho Hong;
Storey, Nathaniel;
Hingley-Wilson, Suzie;
Dühring, Ulf;
Young-Kyung Bae;
Foy, Carole A.;
Braybrook, Julian;
Zeichhardt, Heinz;
Huggett, Jim F.

Publication type:

Article

Pushing the Envelope with Clinical Use of Digital PCR.

Published in:

Clinical Chemistry, 2021, v. 67, n. 7, p. 921, doi. 10.1093/clinchem/hvab082

By:

Huggett, Jim F.;
Devonshire, Alison S.;
Whale, Alexandra S.;
Cowen, Simon;
Foy, Carole A.

Publication type:

Article

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2.

Published in:

Clinical Chemistry, 2020, v. 66, n. 11, p. 1369, doi. 10.1093/clinchem/hvaa214

By:

Huggett, Jim F.;
Benes, Vladimir;
Bustin, Stephen A.;
Garson, Jeremy A.;
Harris, Karthyn;
Kammel, Martin;
Kubista, Mikael;
McHugh, Timothy D.;
Moran-Gilad, Jacob;
Nolan, Tania;
Pfaffl, Michael W.;
Salit, Marc;
Shipley, Greg;
Vallone, Peter M.;
Vandesompele, Jo;
Wittwer, Carl;
Zeichhardt, Heinz

Publication type:

Article

The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020.

Published in:

Clinical Chemistry, 2020, v. 66, n. 8, p. 1012, doi. 10.1093/clinchem/hvaa125

By:

Huggett, Jim F.

Publication type:

Article

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia.

Published in:

Clinical Chemistry, 2017, v. 63, n. 2, p. 525, doi. 10.1373/clinchem.2016.262824

By:

Alikian, Mary;
Whale, Alexandra S.;
Akiki, Susanna;
Piechocki, Kim;
Torrado, Celia;
Myint, Thet;
Cowen, Simon;
Griffiths, Michael;
Reid, Alistair G.;
Apperley, Jane;
White, Helen;
Huggett, Jim F.;
Foroni, Letizia

Publication type:

Article

Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool.

Published in:

Clinical Chemistry, 2015, v. 61, n. 1, p. 79, doi. 10.1373/clinchem.2014.221366

By:

Huggett, Jim F.;
Cowen, Simon;
Foy, Carole A.

Publication type:

Article

Digital PCR as a Novel Technology and Its Potential Implications for Molecular Diagnostics.

Published in:

Clinical Chemistry, 2013, v. 59, n. 12, p. 1691, doi. 10.1373/clinchem.2013.214742

By:

Huggett, Jim F.;
Whale, Alexandra

Publication type:

Article

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Published in:

Clinical Chemistry, 2013, v. 59, n. 6, p. 892, doi. 10.1373/clinchem.2013.206375

By:

Huggett, Jim F.;
Foy, Carole A.;
Benes, Vladimir;
Emslie, Kerry;
Garson, Jeremy A.;
Haynes, Ross;
Hellemans, Jan;
Kubista, Mikael;
Mueller, Reinhold D.;
Nolan, Tania;
Pfaffl, Michael W.;
Shipley, Gregory L.;
Vandesompele, Jo;
Wittwer, Carl T.;
Bustin, Stephen A.

Publication type:

Article

Results of German external quality assessment schemes for SARS-CoV-2 antigen detection.

Published in:

Scientific Reports, 2023, v. 13, n. 1, p. 1, doi. 10.1038/s41598-023-40330-2

By:

Vierbaum, Laura;
Wojtalewicz, Nathalie;
Grunert, Hans-Peter;
Zimmermann, Anika;
Scholz, Annemarie;
Goseberg, Sabine;
Kaiser, Patricia;
Duehring, Ulf;
Drosten, Christian;
Corman, Victor;
Niemeyer, Daniela;
Rabenau, Holger F.;
Obermeier, Martin;
Nitsche, Andreas;
Michel, Janine;
Puyskens, Andreas;
Huggett, Jim F.;
O'Sullivan, Denise M.;
Busby, Eloise;
Cowen, Simon

Publication type:

Article

The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs.

Published in:

Genome Medicine, 2016, v. 8, p. 1, doi. 10.1186/s13073-016-0385-x

By:

Phelan, Jody;
O'Sullivan, Denise M.;
Machado, Diana;
Ramos, Jorge;
Whale, Alexandra S.;
O'Grady, Justin;
Dheda, Keertan;
Campino, Susana;
McNerney, Ruth;
Viveiros, Miguel;
Huggett, Jim F.;
Clark, Taane G.

Publication type:

Article

The pathogen recognition sensor, NOD2, is variably expressed in patients with pulmonary tuberculosis.

Published in:

BMC Infectious Diseases, 2007, v. 7, n. 1, p. 96, doi. 10.1186/1471-2334-7-96

By:

Lala, Sanjay;
Dheda, Keertan;
Jung-Su Chang;
Huggett, Jim F.;
Kim, Louise U.;
Johnson, Margaret A.;
Rook, Graham A.W.;
Keshav, Satish;
ZumlaI, Alimuddin

Publication type:

Article

Evaluation of Digital PCR for Absolute RNA Quantification.

Published in:

PLoS ONE, 2013, v. 8, n. 9, p. 1, doi. 10.1371/journal.pone.0075296

By:

Sanders, Rebecca;
Mason, Deborah J.;
Foy, Carole A.;
Huggett, Jim F.

Publication type:

Article

Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples.

Published in:

PLoS ONE, 2013, v. 8, n. 3, p. 1, doi. 10.1371/journal.pone.0058177

By:

Whale, Alexandra S.;
Cowen, Simon;
Foy, Carole A.;
Huggett, Jim F.

Publication type:

Article

Implications of Storing Urinary DNA from Different Populations for Molecular Analyses.

Published in:

PLoS ONE, 2009, v. 4, n. 9, p. 1, doi. 10.1371/journal.pone.0006985

By:

Cannas, Angela;
Kalunga, Glendah;
Green, Clare;
Calvo, Ludovica;
Katemangwe, Patrick;
Reither, Klaus;
Perkins, Mark D.;
Maboko, Leonard;
Hoelscher, Michael;
Talbot, Elizabeth A.;
Mwaba, Peter;
Zumla, Alimuddin I.;
Girardi, Enrico;
Huggett, Jim F.

Publication type:

Article

Problems of Developing Molecular Diagnostic Tests for Opportunistic Pathogens: The Example of Pneumocystis jirovecii.

Published in:

Journal of Eukaryotic Microbiology, 2006, v. 53, p. S85, doi. 10.1111/j.1550-7408.2006.00182.x

By:

HUGGETT, JIM F.;
MILLER, ROBERT F.;
TAYLOR, MARTIN S.;
COSTELLO, ANTHONY M.;
ZUMLA, ALIMUDDIN

Publication type:

Article

RT-qPCR and RT-Digital PCR: a Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukaemia.

Published in:

Clinical Lymphoma, Myeloma & Leukemia, 2016, v. 16, p. S55, doi. 10.1016/j.clml.2016.07.081

By:

Alikian, Mary;
Whale, Alexandra;
Akiki, Susanna;
Piechocki, Kim;
Torrado, Celia;
Myint, Thet;
Cowen, Simon;
Griffiths, Michael;
Apperley, Jane;
White, Helen;
Huggett, Jim F.;
Foroni, Letizia

Publication type:

Article

Lung Remodeling in Pulmonary Tuberculosis.

Published in:

Journal of Infectious Diseases, 2005, v. 192, n. 7, p. 1201, doi. 10.1086/444545

By:

Dheda, Keertan;
Booth, Helen;
Huggett, Jim F.;
Johnson, Margaret A.;
Zumla, Alimuddin;
Rook, Graham A. W.

Publication type:

Article

P2‐263: CELL‐FREE DNA IN CEREBROSPINAL FLUID: EVALUATING A NEW BIOMARKER FOR ALZHEIMER'S DISEASE.

Published in:

Alzheimer's & Dementia: The Journal of the Alzheimer's Association, 2018, v. 14, p. P777, doi. 10.1016/j.jalz.2018.06.952

By:

Takousis, Petros;
Devonshire, Alison S.;
Redshaw, Nicholas;
von Baumgarten, Louisa;
Whale, Alexandra S.;
Jones, Gerwyn M.;
Huggett, Jim F.;
Alexopoulos, Panagiotis;
Perneczky, Robert

Publication type:

Article

Distribution of Tetrodotoxin in Pacific Oysters (Crassostrea gigas).

Published in:

Marine Drugs, 2021, v. 19, n. 2, p. 84, doi. 10.3390/md19020084

By:

Dhanji-Rapkova, Monika;
Turner, Andrew D.;
Baker-Austin, Craig;
Huggett, Jim F.;
Ritchie, Jennifer M.;
Alvarado, Jorge Lago

Publication type:

Article

Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements.

Published in:

Analytical & Bioanalytical Chemistry, 2022, v. 414, n. 2, p. 791, doi. 10.1007/s00216-021-03712-x

By:

Milavec, Mojca;
Cleveland, Megan H.;
Bae, Young-Kyung;
Wielgosz, Robert I.;
Vonsky, Maxim;
Huggett, Jim F.

Publication type:

Article