Works by Hollfelder, Florian

Results: 55

One-Pot Deracemization of sec-Alcohols: Enantioconvergent Enzymatic Hydrolysis of Alkyl Sulfates Using Stereocomplementary Sulfatases.

Published in:

Angewandte Chemie, 2013, v. 125, n. 11, p. 3359, doi. 10.1002/ange.201209946

By:

Schober, Markus;
Toesch, Michael;
Knaus, Tanja;
Strohmeier, Gernot A.;
van Loo, Bert;
Fuchs, Michael;
Hollfelder, Florian;
Macheroux, Peter;
Faber, Kurt

Publication type:

Article

Mikrotröpfchen in Mikrofluidiksystemen: eine Technik für Entdeckungen in der Chemie und Biologie.

Published in:

Angewandte Chemie, 2010, v. 122, n. 34, p. 5982, doi. 10.1002/ange.200906653

By:

Theberge, Ashleigh B.;
Courtois, Fabienne;
Schaerli, Yolanda;
Fischlechner, Martin;
Abell, Chris;
Hollfelder, Florian;
Huck, Wilhelm T. S.

Publication type:

Article

Off-the-shelf proteins that rival tailor-made antibodies as catalysts.

Published in:

Nature, 1996, v. 383, n. 6595, p. 60, doi. 10.1038/383060a0

By:

Hollfelder, Florian;
Kirby, Anthony J.

Publication type:

Article

An efficient method to assemble linear DNA templates for in vitro screening and selection systems.

Published in:

Nucleic Acids Research, 2009, v. 37, n. 18, p. e122, doi. 10.1093/nar/gkp589

By:

Stein, Viktor;
Hollfelder, Florian

Publication type:

Article

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content.

Published in:

Nature Communications, 2023, v. 14, n. 1, p. 1, doi. 10.1038/s41467-023-40322-w

By:

De Jonghe, Joachim;
Kaminski, Tomasz S.;
Morse, David B.;
Tabaka, Marcin;
Ellermann, Anna L.;
Kohler, Timo N.;
Amadei, Gianluca;
Handford, Charlotte E.;
Findlay, Gregory M.;
Zernicka-Goetz, Magdalena;
Teichmann, Sarah A.;
Hollfelder, Florian

Publication type:

Article

Plakoglobin is a mechanoresponsive regulator of naive pluripotency.

Published in:

Nature Communications, 2023, v. 14, n. 1, p. 1, doi. 10.1038/s41467-023-39515-0

By:

Kohler, Timo N.;
De Jonghe, Joachim;
Ellermann, Anna L.;
Yanagida, Ayaka;
Herger, Michael;
Slatery, Erin M.;
Weberling, Antonia;
Munger, Clara;
Fischer, Katrin;
Mulas, Carla;
Winkel, Alex;
Ross, Connor;
Bergmann, Sophie;
Franze, Kristian;
Chalut, Kevin;
Nichols, Jennifer;
Boroviak, Thorsten E.;
Hollfelder, Florian

Publication type:

Article

An optimized ATP/PPi-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis.

Published in:

Biotechnology Journal, 2007, v. 2, n. 2, p. 232, doi. 10.1002/biot.200600220

By:

Otten, Linda G.;
Schaffer, Michelle L.;
Villiers, Benoit R.M.;
Stachelhaus, Torsten;
Hollfelder, Florian

Publication type:

Article

Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation.

Published in:

Angewandte Chemie International Edition, 2021, v. 60, n. 19, p. 10919, doi. 10.1002/anie.202015834

By:

Biggs, George S.;
Klein, Oskar James;
Maslen, Sarah L.;
Skehel, J. Mark;
Rutherford, Trevor J.;
Freund, Stefan M. V.;
Hollfelder, Florian;
Boss, Sally R.;
Barker, Paul D.

Publication type:

Article

"NAD‐display": Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry.

Published in:

Angewandte Chemie International Edition, 2021, v. 60, n. 16, p. 9015, doi. 10.1002/anie.202013486

By:

Lindenburg, Laurens;
Hollfelder, Florian

Publication type:

Article

Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process.

Published in:

PEDS: Protein Engineering, Design & Selection, 2015, v. 28, n. 9, p. 269, doi. 10.1093/protein/gzv029

By:

Houlihan, Gillian;
Gatti-Lafranconi, Pietro;
Lowe, David;
Hollfelder, Florian

Publication type:

Article

In vitro affinity screening of protein and peptide binders by megavalent bead surface display.

Published in:

PEDS: Protein Engineering, Design & Selection, 2013, v. 26, n. 10, p. 713, doi. 10.1093/protein/gzt039

By:

Diamante, Letizia;
Gatti-Lafranconi, Pietro;
Schaerli, Yolanda;
Hollfelder, Florian

Publication type:

Article

A single mutation in the core domain of the lac repressor reduces leakiness.

Published in:

Microbial Cell Factories, 2013, v. 12, n. 1, p. 1, doi. 10.1186/1475-2859-12-67

By:

Gatti-Lafranconi, Pietro;
Dijkman, Willem P.;
Devenish, Sean R.A.;
Hollfelder, Florian

Publication type:

Article

Combinatorial Synthesis of Structurally Diverse Triazole-Bridged Flavonoid Dimers and Trimers.

Published in:

Molecules, 2016, v. 21, n. 9, p. 1230, doi. 10.3390/molecules21091230

By:

Tze Han Sum;
Tze Jing Sum;
Galloway, Warren R. J. D.;
Collins, Súil;
Twigg, David G.;
Hollfelder, Florian;
Spring, David R.

Publication type:

Article

Long‐Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation.

Published in:

Small, 2019, v. 15, n. 5, p. N.PAG, doi. 10.1002/smll.201804576

By:

Kleine‐Brüggeney, Hans;
van Vliet, Liisa D.;
Mulas, Carla;
Gielen, Fabrice;
Agley, Chibeza C.;
Silva, José C. R.;
Smith, Austin;
Chalut, Kevin;
Hollfelder, Florian

Publication type:

Article

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space.

Published in:

ChemBioChem, 2017, v. 18, n. 11, p. 1001, doi. 10.1002/cbic.201600657

By:

Bayer, Christopher D.;
van Loo, Bert;
Hollfelder, Florian

Publication type:

Article

Efficient Transfection of siRNA by Peptide Dendrimer-Lipid Conjugates.

Published in:

ChemBioChem, 2016, v. 17, n. 23, p. 2223, doi. 10.1002/cbic.201600485

By:

Kwok, Albert;
Eggimann, Gabriela A.;
Heitz, Marc;
Reymond, Jean‐Louis;
Hollfelder, Florian;
Darbre, Tamis

Publication type:

Article

Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease.

Published in:

ChemBioChem, 2015, v. 16, n. 13, p. 1866, doi. 10.1002/cbic.201500295

By:

Shafee, Thomas;
Gatti‐Lafranconi, Pietro;
Minter, Ralph;
Hollfelder, Florian

Publication type:

Article

Flexibility and Reactivity in Promiscuous Enzymes.

Published in:

ChemBioChem, 2013, v. 14, n. 3, p. 285, doi. 10.1002/cbic.201200628

By:

Gatti‐Lafranconi, Pietro;
Hollfelder, Florian

Publication type:

Article

SNAP Dendrimers: Multivalent Protein Display on Dendrimer-Like DNA for Directed Evolution.

Published in:

ChemBioChem, 2011, v. 12, n. 14, p. 2208, doi. 10.1002/cbic.201100240

By:

Kaltenbach, Miriam;
Stein, Viktor;
Hollfelder, Florian

Publication type:

Article

Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA.

Published in:

ChemBioChem, 2009, v. 10, n. 4, p. 671, doi. 10.1002/cbic.200800553

By:

Villiers, Benoit R. M.;
Hollfelder, Florian

Publication type:

Article

Relating Chemical and Biological Diversity Space: A Tunable System for Efficient Gene Transfection.

Published in:

ChemBioChem, 2008, v. 9, n. 12, p. 1960, doi. 10.1002/cbic.200800003

By:

Van Vliet, Liisa D.;
Chapman, Michael R.;
Avenier, Frédéric;
Kitson, Chris Z.;
Hollfelder, Florian

Publication type:

Article

The Human Histone Acetyltransferase P/CAF is a Promiscuous Histone Propionyltransferase.

Published in:

ChemBioChem, 2008, v. 9, n. 4, p. 499, doi. 10.1002/cbic.200700556

By:

Leemhuis, Hans;
Packman, Len C.;
Nightingale, Karl P.;
Hollfelder, Florian

Publication type:

Article

An Integrated Device for Monitoring Time-Dependent in vitro Expression From Single Genes in Picolitre Droplets.

Published in:

ChemBioChem, 2008, v. 9, n. 3, p. 439, doi. 10.1002/cbic.200700536

By:

Courtois, Fabienne;
Olguin, Luis F.;
Whyte, Graeme;
Bratton, Daniel;
Huck, Wilhelm T. S.;
Abell, Chris;
Hollfelder, Florian

Publication type:

Article

Cover Picture: A Covalent Chemical Genotype-Phenotype Linkage for in vitro Protein Evolution (ChemBioChem 18/2007).

Published in:

ChemBioChem, 2007, v. 8, n. 18, p. 2173, doi. 10.1002/cbic.200790064

By:

Stein, Viktor;
Sielaff, India;
Johnsson, Kai;
Hollfelder, Florian

Publication type:

Article

A Covalent Chemical Genotype-Phenotype Linkage for in vitro Protein Evolution.

Published in:

ChemBioChem, 2007, v. 8, n. 18, p. 2191, doi. 10.1002/cbic.200700459

By:

Stein, Viktor;
Sielaff, India;
Johnsson, Kai;
Hollfelder, Florian

Publication type:

Article

European Symposium of Bio-Organic Chemistry 2003 (ESBOC): The Evolution of Catalysis.

Published in:

ChemBioChem, 2004, v. 5, n. 2, p. 241, doi. 10.1002/cbic.200300796

By:

Ariel Lindner;
Florian Hollfelder

Publication type:

Article

Biochemistry: Enzymes under the nanoscope.

Published in:

Nature, 2008, v. 456, n. 7218, p. 45, doi. 10.1038/456045a

By:

Kirby, Anthony J.;
Hollfelder, Florian

Publication type:

Article

Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli.

Published in:

Microbial Biotechnology, 2022, v. 15, n. 11, p. 2845, doi. 10.1111/1751-7915.14144

By:

Eenink, Bernard D. G.;
Kaminski, Tomasz S.;
Bornberg‐Bauer, Erich;
Jose, Joachim;
Hollfelder, Florian;
van Loo, Bert

Publication type:

Article

Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay.

Published in:

ChemBioChem, 2021, v. 22, n. 23, p. 3292, doi. 10.1002/cbic.202100322

By:

Klaus, Miriam;
Zurek, Paul Jannis;
Kaminski, Tomasz S.;
Pushpanath, Ahir;
Neufeld, Katharina;
Hollfelder, Florian

Publication type:

Article

Evolution of enzyme catalysts caged in biomimetic gel-shell beads.

Published in:

Nature Chemistry, 2014, v. 6, n. 9, p. 791, doi. 10.1038/nchem.1996

By:

Fischlechner, Martin;
Schaerli, Yolanda;
Mohamed, Mark F.;
Patil, Santosh;
Abell, Chris;
Hollfelder, Florian

Publication type:

Article

Intramolecular general acid catalysis of sulfate transfer — Nucleophilic attack by oxyanions on the SO3– group.

Published in:

Canadian Journal of Chemistry, 2005, v. 83, n. 9, p. 1629, doi. 10.1139/V05-172

By:

Kirby, Anthony J.;
Gesser, José Carlos;
Hollfelder, Florian;
Priebe, Jacks P.;
Nome, Faruk

Publication type:

Article

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity.

Published in:

PLoS Genetics, 2016, v. 12, n. 10, p. 1, doi. 10.1371/journal.pgen.1006305

By:

Kaltenbach, Miriam;
Emond, Stephane;
Hollfelder, Florian;
Tokuriki, Nobuhiko

Publication type:

Article

One-Pot Deracemization of sec-Alcohols: Enantioconvergent Enzymatic Hydrolysis of Alkyl Sulfates Using Stereocomplementary Sulfatases.

Published in:

Angewandte Chemie International Edition, 2013, v. 52, n. 11, p. 3277, doi. 10.1002/anie.201209946

By:

Schober, Markus;
Toesch, Michael;
Knaus, Tanja;
Strohmeier, Gernot A.;
van Loo, Bert;
Fuchs, Michael;
Hollfelder, Florian;
Macheroux, Peter;
Faber, Kurt

Publication type:

Article

Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology.

Published in:

Angewandte Chemie International Edition, 2010, v. 49, n. 34, p. 5846, doi. 10.1002/anie.200906653

By:

Theberge, Ashleigh B.;
Courtois, Fabienne;
Schaerli, Yolanda;
Fischlechner, Martin;
Abell, Chris;
Hollfelder, Florian;
Huck, Wilhelm T. S.

Publication type:

Article

Efficient Catalytic Promiscuity for Chemically Distinct Reactions.

Published in:

Angewandte Chemie International Edition, 2009, v. 48, n. 20, p. 3692, doi. 10.1002/anie.200805843

By:

Babtie, Ann C.;
Bandyopadhyay, Subhajit;
Olguin, Luis F.;
Hollfelder, Florian

Publication type:

Article

High-throughput total RNA sequencing in single cells using VASA-seq.

Published in:

Nature Biotechnology, 2022, v. 40, n. 12, p. 1780, doi. 10.1038/s41587-022-01361-8

By:

Salmen, Fredrik;
De Jonghe, Joachim;
Kaminski, Tomasz S.;
Alemany, Anna;
Parada, Guillermo E.;
Verity-Legg, Joe;
Yanagida, Ayaka;
Kohler, Timo N.;
Battich, Nicholas;
van den Brekel, Floris;
Ellermann, Anna L.;
Arias, Alfonso Martinez;
Nichols, Jennifer;
Hemberg, Martin;
Hollfelder, Florian;
van Oudenaarden, Alexander

Publication type:

Article

Combining Medium Effects and Cofactor Catalysis: Metal-Coordinated Synzymes Accelerate Phosphate Transfer by 108.

Published in:

Chemistry - A European Journal, 2009, v. 15, n. 45, p. 12371, doi. 10.1002/chem.200802616

By:

Avenier, Frédéric;
Hollfelder, Florian

Publication type:

Article

Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation.

Published in:

Angewandte Chemie, 2021, v. 133, n. 19, p. 11014, doi. 10.1002/ange.202015834

By:

Biggs, George S.;
Klein, Oskar James;
Maslen, Sarah L.;
Skehel, J. Mark;
Rutherford, Trevor J.;
Freund, Stefan M. V.;
Hollfelder, Florian;
Boss, Sally R.;
Barker, Paul D.

Publication type:

Article

"NAD‐display": Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry.

Published in:

Angewandte Chemie, 2021, v. 133, n. 16, p. 9097, doi. 10.1002/ange.202013486

By:

Lindenburg, Laurens;
Hollfelder, Florian

Publication type:

Article

Mapping catalytic promiscuity in the alkaline phosphatase superfamily.

Published in:

Pure & Applied Chemistry, 2009, v. 81, n. 4, p. 731, doi. 10.1351/PAC-CON-08-10-20

By:

Jonas, Stefanie;
Hollfelder, Florian

Publication type:

Article

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.

Published in:

Nucleic Acids Research, 2020, v. 48, n. 11, p. e63, doi. 10.1093/nar/gkaa270

By:

Lindenburg, Laurens;
Huovinen, Tuomas;
van de Wiel, Kayleigh;
Herger, Michael;
Snaith, Michael R;
Hollfelder, Florian

Publication type:

Article

Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification.

Published in:

Nucleic Acids Research, 2010, v. 38, n. 22, p. e201, doi. 10.1093/nar/gkq795

By:

Schaerli, Yolanda;
Stein, Viktor;
Spiering, Michelle M.;
Benkovic, Stephen J.;
Abell, Chris;
Hollfelder, Florian

Publication type:

Article

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics.

Published in:

Nature Communications, 2015, v. 6, n. 12, p. 10008, doi. 10.1038/ncomms10008

By:

Colin, Pierre-Yves;
Kintses, Balint;
Gielen, Fabrice;
Miton, Charlotte M.;
Fischer, Gerhard;
Mohamed, Mark F.;
Hyvönen, Marko;
Morgavi, Diego P.;
Janssen, Dick B;
Hollfelder, Florian

Publication type:

Article

Investigating host-microbiome interactions by droplet based microfluidics.

Published in:

Microbiome, 2020, v. 8, n. 1, p. N.PAG, doi. 10.1186/s40168-020-00911-z

By:

Tauzin, Alexandra S.;
Pereira, Mariana Rangel;
Van Vliet, Liisa D.;
Colin, Pierre-Yves;
Laville, Elisabeth;
Esque, Jeremy;
Laguerre, Sandrine;
Henrissat, Bernard;
Terrapon, Nicolas;
Lombard, Vincent;
Leclerc, Marion;
Doré, Joël;
Hollfelder, Florian;
Potocki-Veronese, Gabrielle

Publication type:

Article

A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis.

Published in:

Scientific Reports, 2016, p. 36391, doi. 10.1038/srep36391

By:

Mankowska, Sylwia A.;
Gatti-Lafranconi, Pietro;
Chodorge, Matthieu;
Sridharan, Sudharsan;
Minter, Ralph R.;
Hollfelder, Florian

Publication type:

Article

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution.

Published in:

Nature Communications, 2020, v. 11, n. 1, p. N.PAG, doi. 10.1038/s41467-020-19687-9

By:

Zurek, Paul Jannis;
Knyphausen, Philipp;
Neufeld, Katharina;
Pushpanath, Ahir;
Hollfelder, Florian

Publication type:

Article

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis.

Published in:

Nature Communications, 2020, v. 11, n. 1, p. 1, doi. 10.1038/s41467-020-17061-3

By:

Emond, Stephane;
Petek, Maya;
Kay, Emily J.;
Heames, Brennen;
Devenish, Sean R. A.;
Tokuriki, Nobuhiko;
Hollfelder, Florian

Publication type:

Article

Mutations in Mll2, an H3K4 Methyltransferase, Result in Insulin Resistance and Impaired Glucose Tolerance in Mice.

Published in:

PLoS ONE, 2013, v. 8, n. 6, p. 1, doi. 10.1371/journal.pone.0061870

By:

Goldsworthy, Michelle;
Absalom, Nathan L.;
Schröter, David;
Matthews, Helen C.;
Bogani, Debora;
Moir, Lee;
Long, Anna;
Church, Christopher;
Hugill, Alison;
Anstee, Quentin M.;
Goldin, Rob;
Thursz, Mark;
Hollfelder, Florian;
Cox, Roger D.

Publication type:

Article

Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity.

Published in:

FEBS Journal, 2008, v. 275, n. 22, p. 5635, doi. 10.1111/j.1742-4658.2008.06689.x

By:

Leemhuis, Hans;
Nightingale, Karl P.;
Hollfelder, Florian

Publication type:

Article

Reverse evolution leads to genotypic incompatibility despite functional and active-site convergence.

Published in:

eLife, 2015, p. 1, doi. 10.7554/eLife.06492

By:

Kaltenbach, Miriam;
Jackson, Colin J.;
Campbell, Eleanor C.;
Hollfelder, Florian;
Nobuhiko Tokuriki

Publication type:

Article