Works by Gasser, Brigitte

Results: 57

Sample preparation workflow for the liquid chromatography tandem mass spectrometry based analysis of nicotinamide adenine dinucleotide phosphate cofactors in yeast†.

Published in:

Journal of Separation Science, 2014, v. 37, n. 16, p. 2185, doi. 10.1002/jssc.201400290

By:

Ortmayr, Karin;
Nocon, Justyna;
Gasser, Brigitte;
Mattanovich, Diethard;
Hann, Stephan;
Koellensperger, Gunda

Publication type:

Article

U13 C cell extract of Pichia pastoris - a powerful tool for evaluation of sample preparation in metabolomics.

Published in:

Journal of Separation Science, 2012, v. 35, n. 22, p. 3091, doi. 10.1002/jssc.201200447

By:

Neubauer, Stefan;
Haberhauer-Troyer, Christina;
Klavins, Kristaps;
Russmayer, Hannes;
Steiger, Matthias G.;
Gasser, Brigitte;
Sauer, Michael;
Mattanovich, Diethard;
Hann, Stephan;
Koellensperger, Gunda

Publication type:

Article

The vitamin-sensitive promoter P THI11 enables pre-defined autonomous induction of recombinant protein production in Pichia pastoris.

Published in:

Biotechnology & Bioengineering, 2016, v. 113, n. 12, p. 2633, doi. 10.1002/bit.26041

By:

Landes, Nils;
Gasser, Brigitte;
Vorauer‐Uhl, Karola;
Lhota, Gabriele;
Mattanovich, Diethard;
Maurer, Michael

Publication type:

Article

Reverse engineering of protein secretion by uncoupling of cell cycle phases from growth.

Published in:

Biotechnology & Bioengineering, 2011, v. 108, n. 10, p. 2403, doi. 10.1002/bit.23198

By:

Buchetics, Markus;
Dragosits, Martin;
Maurer, Michael;
Rebnegger, Corinna;
Porro, Danilo;
Sauer, Michael;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production in Pichia pastoris.

Published in:

Biotechnology & Bioengineering, 2010, v. 105, n. 3, p. 543, doi. 10.1002/bit.22573

By:

Stadlmayr, Gerhard;
Benakovitsch, Katrin;
Gasser, Brigitte;
Mattanovich, Diethard;
Sauer, Michael

Publication type:

Article

Engineering of Pichia pastoris for improved production of antibody fragments.

Published in:

Biotechnology & Bioengineering, 2006, v. 94, n. 2, p. 353, doi. 10.1002/bit.20851

By:

Gasser, Brigitte;
Maurer, Michael;
Gach, Johannes;
Kunert, Renate;
Mattanovich, Diethard

Publication type:

Article

Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris.

Published in:

Biotechnology & Bioengineering, 2004, v. 85, n. 4, p. 367, doi. 10.1002/bit.10904

By:

Hubertus Hohenblum;
Brigitte Gasser;
Michael Maurer;
Nicole Borth;
Diethard Mattanovich

Publication type:

Article

Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii.

Published in:

Current Genetics, 2021, v. 67, n. 4, p. 641, doi. 10.1007/s00294-021-01165-4

By:

Barbay, Diane;
Mačáková, Monika;
Sützl, Leander;
De, Sonakshi;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

The bud tip is the cellular hot spot of protein secretion in yeasts.

Published in:

Applied Microbiology & Biotechnology, 2016, v. 100, n. 18, p. 8159, doi. 10.1007/s00253-016-7674-6

By:

Puxbaum, Verena;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris.

Published in:

Applied Microbiology & Biotechnology, 2016, v. 100, n. 13, p. 5955, doi. 10.1007/s00253-016-7363-5

By:

Nocon, Justyna;
Steiger, Matthias;
Mairinger, Teresa;
Hohlweg, Jonas;
Rußmayer, Hannes;
Hann, Stephan;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris.

Published in:

Applied Microbiology & Biotechnology, 2015, v. 99, n. 7, p. 2925, doi. 10.1007/s00253-015-6470-z

By:

Puxbaum, Verena;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Identification and deletion of the major secreted protein of Pichia pastoris.

Published in:

Applied Microbiology & Biotechnology, 2013, v. 97, n. 3, p. 1241, doi. 10.1007/s00253-012-4260-4

By:

Heiss, Silvia;
Maurer, Michael;
Hahn, Rainer;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Production of recombinant proteins and metabolites in yeasts.

Published in:

Applied Microbiology & Biotechnology, 2011, v. 89, n. 4, p. 939, doi. 10.1007/s00253-010-3019-z

By:

Porro, Danilo;
Gasser, Brigitte;
Fossati, Tiziana;
Maurer, Michael;
Branduardi, Paola;
Sauer, Michael;
Mattanovich, Diethard

Publication type:

Article

Digital humanities and digital social reading.

Published in:

Digital Scholarship in the Humanities, 2021, v. 36, p. ii230, doi. 10.1093/llc/fqab020

By:

Rebora, Simone;
Boot, Peter;
Pianzola, Federico;
Gasser, Brigitte;
Herrmann, J Berenike;
Kraxenberger, Maria;
Kuijpers, Moniek M;
Lauer, Gerhard;
Lendvai, Piroska;
Messerli, Thomas C;
Sorrentino, Pasqualina

Publication type:

Article

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications.

Published in:

Biotechnology & Bioengineering, 2020, v. 117, n. 5, p. 1394, doi. 10.1002/bit.27303

By:

Zavec, Domen;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Engineering of alcohol dehydrogenase 2 hybrid‐promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol.

Published in:

Biotechnology & Bioengineering, 2019, v. 116, n. 10, p. 2674, doi. 10.1002/bit.27095

By:

Ergün, Burcu Gündüz;
Gasser, Brigitte;
Mattanovich, Diethard;
Çalık, Pınar

Publication type:

Article

Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated GTH1 promoter of Pichia pastoris.

Published in:

Biotechnology & Bioengineering, 2018, v. 115, n. 10, p. 2479, doi. 10.1002/bit.26800

By:

Prielhofer, Roland;
Reichinger, Michaela;
Wagner, Nina;
Claes, Katrien;
Kiziak, Christoph;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris.

Published in:

Biotechnology & Bioengineering, 2017, v. 114, n. 10, p. 2319, doi. 10.1002/bit.26363

By:

Ata, Özge;
Prielhofer, Roland;
Gasser, Brigitte;
Mattanovich, Diethard;
Çalık, Pınar

Publication type:

Article

Expression and characterization of cold-adapted xylanase Xyl-L in Pichia pastoris for xylooligosaccharide (XOS) preparation.

Published in:

Microbial Cell Factories, 2025, v. 24, n. 1, p. 1, doi. 10.1186/s12934-025-02690-4

By:

Rodríguez, Sebastián;
González, Carolina;
Reyes-Godoy, José Pablo;
Gasser, Brigitte;
Andrews, Barbara;
Asenjo, Juan A.

Publication type:

Article

Impact of Oxygen Availability on the Organelle‐Specific Redox Potentials and Stress in Recombinant Protein Producing Komagataella phaffii.

Published in:

Microbial Biotechnology, 2025, v. 18, n. 2, p. 1, doi. 10.1111/1751-7915.70106

By:

Kostopoulou, Aliki;
Rebnegger, Corinna;
Ferrero‐Bordera, Borja;
Mattanovich, Matthias;
Maaß, Sandra;
Becher, Dörte;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Characterising the metabolic rewiring of extremely slow growing Komagataella phaffii.

Published in:

Microbial Biotechnology, 2024, v. 17, n. 1, p. 1, doi. 10.1111/1751-7915.14386

By:

Coltman, Benjamin Luke;
Rebnegger, Corinna;
Gasser, Brigitte;
Zanghellini, Jürgen

Publication type:

Article

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time.

Published in:

Microbial Biotechnology, 2020, v. 13, n. 2, p. 479, doi. 10.1111/1751-7915.13499

By:

Burgard, Jonas;
Grünwald‐Gruber, Clemens;
Altmann, Friedrich;
Zanghellini, Jürgen;
Valli, Minoska;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.

Published in:

Microbial Biotechnology, 2016, v. 9, n. 3, p. 355, doi. 10.1111/1751-7915.12350

By:

Ben Azoun, Safa;
Belhaj, Aicha Eya;
Göngrich, Rebecca;
Gasser, Brigitte;
Kallel, Héla

Publication type:

Article

technoYeast: New technologies and developments from the Yeast world.

Published in:

Yeast, 2022, v. 39, n. 6, p. 353, doi. 10.1002/yea.3805

By:

Gasser, Brigitte;
Petrovič, Uroš

Publication type:

Article

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris.

Published in:

BMC Systems Biology, 2017, v. 11, p. 1, doi. 10.1186/s12918-017-0492-3

By:

Prielhofer, Roland;
Barrero, Juan J.;
Steuer, Stefanie;
Gassler, Thomas;
Zahrl, Richard;
Baumann, Kristin;
Sauer, Michael;
Mattanovich, Diethard;
Gasser, Brigitte;
Marx, Hans

Publication type:

Article

A multi-level study of recombinant Pichia pastoris in different oxygen conditions.

Published in:

BMC Systems Biology, 2010, v. 4, p. 141, doi. 10.1186/1752-0509-4-141

By:

Baumann, Kristin;
Carnicer, Marc;
Dragosits, Martin;
Graf, Alexandra B.;
Stadlmann, Johannes;
Jouhten, Paula;
Maaheimo, Hannu;
Gasser, Brigitte;
Albiol, Joan;
Mattanovich, Diethard;
Ferrer, Pau

Publication type:

Article

Yeast biotechnology: teaching the old dog new tricks.

Published in:

Microbial Cell Factories, 2014, v. 13, n. 1, p. 1, doi. 10.1186/1475-2859-13-34

By:

Mattanovich, Diethard;
Sauer, Michael;
Gasser, Brigitte

Publication type:

Article

Repressible promoters - A novel tool to generate conditional mutants in Pichia pastoris.

Published in:

Microbial Cell Factories, 2013, v. 12, n. 1, p. 1, doi. 10.1186/1475-2859-12-6

By:

Delic, Marizela;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.

Published in:

Microbial Cell Factories, 2013, v. 12, n. 1, p. 1, doi. 10.1186/1475-2859-12-5

By:

Prielhofer, Roland;
Maurer, Michael;
Klein, Joachim;
Wenger, Jana;
Kiziak, Christoph;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.

Published in:

Microbial Cell Factories, 2008, v. 7, p. 1, doi. 10.1186/1475-2859-7-11

By:

Gasser, Brigitte;
Saloheimo, Markku;
Rinas, Ursula;
Dragosits, Martin;
Rodríguez-Carmona, Escarlata;
Baumann, Kristin;
Giuliani, Maria;
Parrilli, Ermenegilda;
Branduardi, Paola;
Lang, Christine;
Porro, Danilo;
Ferrer, Pau;
Tutino, Maria Luisa;
Mattanovich, Diethard;
Villaverde, Antonio

Publication type:

Article

Analysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1).

Published in:

2006

By:

Resina, David;
Cos, Oriol;
Gasser, Brigitte;
Mauer, Michael;
Marx, Hans;
Sauer, Michael;
Valero, Francisco;
Mattanovich, Diethard;
Ferrer, Pau

Publication type:

Abstract

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation.

Published in:

Microbial Cell Factories, 2004, v. 3, p. 1, doi. 10.1186/1475-2859-3-17

By:

Sauer, Michael;
Branduardi, Paola;
Gasser, Brigitte;
Valli, Minoska;
Maurer, Michael;
Porro, Danilo;
Mattanovich, Diethard

Publication type:

Article

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level.

Published in:

BMC Genomics, 2015, v. 16, n. 1, p. 1, doi. 10.1186/s12864-015-1393-8

By:

Prielhofer, Roland;
Cartwright, Stephanie P.;
Graf, Alexandra B.;
Valli, Minoska;
Bill, Roslyn M.;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation.

Published in:

Molecular & Cellular Biology, 2018, v. 38, n. 2, p. 1, doi. 10.1128/MCB.00398-17

By:

Heistinger, Lina;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Customizing amino acid metabolism of Pichia pastoris for recombinant protein production.

Published in:

Biotechnology Journal, 2023, v. 18, n. 12, p. 1, doi. 10.1002/biot.202300033

By:

Rußmayer, Hannes;
Buchetics, Markus;
Mattanovich, Matthias;
Neubauer, Stefan;
Steiger, Matthias;
Graf, Alexandra B.;
Koellensperger, Gunda;
Hann, Stephan;
Sauer, Michael;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

The Degree and Length of O‐Glycosylation of Recombinant Proteins Produced in Pichia pastoris Depends on the Nature of the Protein and the Process Type.

Published in:

Biotechnology Journal, 2021, v. 16, n. 3, p. 1, doi. 10.1002/biot.202000266

By:

Radoman, Bojana;
Grünwald‐Gruber, Clemens;
Schmelzer, Bernhard;
Zavec, Domen;
Gasser, Brigitte;
Altmann, Friedrich;
Mattanovich, Diethard

Publication type:

Article

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures.

Published in:

Microbial Cell Factories, 2017, v. 16, p. 1, doi. 10.1186/s12934-017-0730-9

By:

Burgard, Jonas;
Valli, Minoska;
Graf, Alexandra B.;
Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Functional inclusion bodies produced in the yeast Pichia pastoris.

Published in:

Microbial Cell Factories, 2016, v. 15, p. 1, doi. 10.1186/s12934-016-0565-9

By:

Rueda, Fabián;
Gasser, Brigitte;
Sánchez-Chardi, Alejandro;
Roldán, Mònica;
Villegas, Sandra;
Puxbaum, Verena;
Ferrer-Miralles, Neus;
Unzueta, Ugutz;
Vázquez, Esther;
Garcia-Fruitós, Elena;
Mattanovich, Diethard;
Villaverde, Antonio

Publication type:

Article

Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.

Published in:

Microbial Cell Factories, 2015, v. 14, p. 1, doi. 10.1186/s12934-015-0387-1

By:

Gasser, Brigitte;
Steiger, Matthias G.;
Mattanovich, Diethard

Publication type:

Article

Antibody production with yeasts and filamentous fungi: on the road to large scale?

Published in:

Biotechnology Letters, 2007, v. 29, n. 2, p. 201, doi. 10.1007/s10529-006-9237-x

By:

Gasser, Brigitte;
Mattanovich, Diethard

Publication type:

Article

Biochemical analysis of Komagataella phaffii oxidative folding proposes novel regulatory mechanisms of disulfide bond formation in yeast.

Published in:

Scientific Reports, 2023, v. 13, n. 1, p. 1, doi. 10.1038/s41598-023-41375-z

By:

Palma, Arianna;
Rettenbacher, Lukas A.;
Moilanen, Antti;
Saaranen, Mirva;
Pacheco-Martinez, Christian;
Gasser, Brigitte;
Ruddock, Lloyd

Publication type:

Article

Systematic sequence engineering enhances the induction strength of the glucose-regulated GTH1 promoter of Komagataella phaffii.

Published in:

Nucleic Acids Research, 2023, v. 51, n. 20, p. 11358, doi. 10.1093/nar/gkad752

By:

Flores-Villegas, Mirelle;
Rebnegger, Corinna;
Kowarz, Viktoria;
Prielhofer, Roland;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis.

Published in:

Biotechnology Progress, 2011, v. 27, n. 1, p. 38, doi. 10.1002/btpr.524

By:

Dragosits, Martin;
Frascotti, Gianni;
Bernard-Granger, Lise;
Vázquez, Felícitas;
Giuliani, Maria;
Baumann, Kristin;
Rodríguez-Carmona, Escarlata;
Tokkanen, Jaana;
Parrilli, Ermenegilda;
Wiebe, Marilyn G.;
Kunert, Renate;
Maurer, Michael;
Gasser, Brigitte;
Sauer, Michael;
Branduardi, Paola;
Pakula, Tiina;
Saloheimo, Markku;
Penttilä, Merja;
Ferrer, Pau;
Tutino, Maria Luisa

Publication type:

Article

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris.

Published in:

PLoS ONE, 2011, v. 6, n. 8, p. 1, doi. 10.1371/journal.pone.0022577

By:

Bai, Jiangping;
Swartz, Douglas J.;
Protasevich, Irina I.;
Brouillette, Christie G.;
Harrell, Patina M.;
Hildebrandt, Ellen;
Gasser, Brigitte;
Mattanovich, Diethard;
Ward, Andrew;
Chang, Geoffrey;
Urbatsch, Ina L.

Publication type:

Article

Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris.

Published in:

Biotechnology Journal, 2017, v. 12, n. 5, p. n/a, doi. 10.1002/biot.201600584

By:

Marsalek, Lukas;
Gruber, Clemens;
Altmann, Friedrich;
Aleschko, Markus;
Mattanovich, Diethard;
Gasser, Brigitte;
Puxbaum, Verena

Publication type:

Article

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.

Published in:

Biotechnology Journal, 2014, v. 9, n. 4, p. 511, doi. 10.1002/biot.201300334

By:

Rebnegger, Corinna;
Graf, Alexandra B.;
Valli, Minoska;
Steiger, Matthias G.;
Gasser, Brigitte;
Maurer, Michael;
Mattanovich, Diethard

Publication type:

Article

Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins.

Published in:

Biotechnology Journal, 2014, v. 9, n. 4, p. 526, doi. 10.1002/biot.201300305

By:

Maccani, Andreas;
Landes, Nils;
Stadlmayr, Gerhard;
Maresch, Daniel;
Leitner, Christian;
Maurer, Michael;
Gasser, Brigitte;
Ernst, Wolfgang;
Kunert, Renate;
Mattanovich, Diethard

Publication type:

Article

Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production.

Published in:

Biotechnology Journal, 2010, v. 5, n. 7, p. 705, doi. 10.1002/biot.201000078

By:

Sohn, Seung Bum;
Graf, Alexandra B.;
Kim, Tae Yong;
Gasser, Brigitte;
Maurer, Michael;
Ferrer, Pau;
Mattanovich, Diethard;
Lee, Sang Yup

Publication type:

Article

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris.

Published in:

Microbial Cell Factories, 2019, v. 18, n. 1, p. N.PAG, doi. 10.1186/s12934-019-1155-4

By:

Marsalek, Lukas;
Puxbaum, Verena;
Buchetics, Markus;
Mattanovich, Diethard;
Gasser, Brigitte

Publication type:

Article

Accurate quantification of the redox-sensitive GSH/GSSG ratios in the yeast Pichia pastoris by HILIC-MS/MS.

Published in:

Analytical & Bioanalytical Chemistry, 2013, v. 405, n. 6, p. 2031, doi. 10.1007/s00216-012-6620-4

By:

Haberhauer-Troyer, Christina;
Delic, Marizela;
Gasser, Brigitte;
Mattanovich, Diethard;
Hann, Stephan;
Koellensperger, Gunda

Publication type:

Article