Works matching AU Fernandez-Lafuente, Roberto

Results: 145

Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of β-lactam antibiotics.

Published in:

Biotechnology & Bioengineering, 2009, v. 103, n. 3, p. 472, doi. 10.1002/bit.22264

By:

Romero, Oscar;
Vergara, José;
Fernández-Lafuente, Roberto;
Guisán, José Manuel;
Illanes, Andrés;
Wilson, Lorena

Publication type:

Article

Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium.

Published in:

Biotechnology & Bioengineering, 2007, v. 97, n. 2, p. 242, doi. 10.1002/bit.21230

By:

Fernandez-Lorente, Gloria;
Palomo, Jose M.;
Cabrera, Zaida;
Fernandez-Lafuente, Roberto;
Guisán, José M.

Publication type:

Article

Purification of different lipases from Aspergillus niger by using a highly selective adsorption on hydrophobic supports.

Published in:

Biotechnology & Bioengineering, 2005, v. 92, n. 6, p. 773, doi. 10.1002/bit.20656

By:

Fernández-Lorente, Gloria;
Ortiz, Claudia;
Segura, Rosa L.;
Fernández-Lafuente, Roberto;
Guisán, Jose M.;
Palomo, Jose M.

Publication type:

Article

Stabilization of enzymes by multipoint immobilization of thiolated proteins on new epoxy-thiol supports.

Published in:

Biotechnology & Bioengineering, 2005, v. 90, n. 5, p. 597, doi. 10.1002/bit.20452

By:

Grazú, Valeria;
Abian, Olga;
Mateo, Cesar;
Batista-Viera, Franciso;
Fernández-Lafuente, Roberto;
Guisán, José Manuel

Publication type:

Article

Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: Evaluation of a novel biocatalyst in organic media.

Published in:

Biotechnology & Bioengineering, 2004, v. 86, n. 5, p. 558, doi. 10.1002/bit.20107

By:

Wilson, Lorena;
Illanes, Andrés;
Pessela, Benevides C. C.;
Abian, Olga;
Fernández-Lafuente, Roberto;
Guisán, José M.

Publication type:

Article

Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties.

Published in:

Biotechnology & Bioengineering, 2003, v. 82, n. 2, p. 232, doi. 10.1002/bit.10560

By:

Gloria Fernández-Lorente;
José M. Palomo;
Manuel Fuentes;
Cesar Mateo;
José M. Guisán;
Roberto Fernández-Lafuente

Publication type:

Article

Enzymatic synthesis of amoxicillin: Avoiding limitations of the mechanistic approach for reaction kinetics.

Published in:

Biotechnology & Bioengineering, 2002, v. 80, n. 6, p. 622, doi. 10.1002/bit.10417

By:

Gonçalves, Luciana R.B.;
Sousa, Ruy;
Fernandez-Lafuente, Roberto;
Guisan, Jose M.;
Giordano, Raquel L.C.;
Giordano, Roberto C.

Publication type:

Article

One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports.

Published in:

Biotechnology & Bioengineering, 2001, v. 76, n. 3, p. 269, doi. 10.1002/bit.10019

By:

Mateo, Cesar;
Fernández-Lorente, Gloria;
Cortés, Estrella;
Garcia, José L.;
Fernández-Lafuente, Roberto;
Guisan, José M.

Publication type:

Article

Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus.

Published in:

Applied Microbiology & Biotechnology, 2008, v. 80, n. 1, p. 49, doi. 10.1007/s00253-008-1521-3

By:

Bolivar, Juan;
Cava, Felipe;
Mateo, Cesar;
Rocha-Martín, Javier;
Guisán, Jose;
Berenguer, José;
Fernandez-Lafuente, Roberto

Publication type:

Article

Enzymatic synthesis of cephalosporins. The immobilized acylase from Arthrobacter viscosus: A new useful biocatalyst.

Published in:

Applied Microbiology & Biotechnology, 2007, v. 77, n. 3, p. 579, doi. 10.1007/s00253-007-1186-3

By:

Terreni, Marco;
Ubiali, Daniela;
Bavaro, Teodora;
Pregnolato, Massimo;
Fernández-Lafuente, Roberto;
Guisán, José M.

Publication type:

Article

Biotechnological Applications of Proteases in Food Technology.

Published in:

Comprehensive Reviews in Food Science & Food Safety, 2018, v. 17, n. 2, p. 412, doi. 10.1111/1541-4337.12326

By:

Tavano, Olga Luisa;
Berenguer‐Murcia, Angel;
Secundo, Francesco;
Fernandez‐Lafuente, Roberto

Publication type:

Article

Purification, stabilization, and concentration of very weak protein-protein complexes: Shifting the association equilibrium via complex selective adsorption on lowly activated supports.

Published in:

Proteomics, 2005, v. 5, n. 16, p. 4062, doi. 10.1002/pmic.200401270

By:

Manuel Fuentes;
Cesar Mateo;
Benevides 4;C. 4;C. Pessela;
Jose 4;M. Guisán;
Roberto Fernandez-Lafuente

Publication type:

Article

Determination of protein-protein interactions through aldehyde-dextran intermolecular cross-linking.

Published in:

Proteomics, 2004, v. 4, n. 9, p. 2602, doi. 10.1002/pmic.200300766

By:

Manuel Fuentes;
Rosa L. Segura;
Olga Abian;
Lorena Betancor;
Aurelio Hidalgo;
Cesar Mateo;
Roberto Fernandez-Lafuente;
Jose M. Guisan

Publication type:

Article

Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities.

Published in:

Chemical Record, 2016, v. 16, n. 3, p. 1436, doi. 10.1002/tcr.201600007

By:

Rueda, Nazzoly;
dos Santos, Jose C. S.;
Ortiz, Claudia;
Torres, Rodrigo;
Barbosa, Oveimar;
Rodrigues, Rafael C.;
Berenguer‐Murcia, Ángel;
Fernandez‐Lafuente, Roberto

Publication type:

Article

Immobilized Lipases in the Synthesis of Short-Chain Esters: An Overview of Constraints and Perspectives.

Published in:

Catalysts (2073-4344), 2025, v. 15, n. 4, p. 375, doi. 10.3390/catal15040375

By:

Sousa, Ronaldo Rodrigues de;
dos Santos, Michelle M.;
Medeiros, Matheus W. R.;
Manoel, Evelin A.;
Berenguer-Murcia, Ángel;
Freire, Denise Maria Guimarães;
Fernandez-Lafuente, Roberto;
Ferreira-Leitão, Viridiana Santana

Publication type:

Article

Optimization of the Full Hydrolysis of Babassu Oil by Combi-Lipases.

Published in:

Catalysts (2073-4344), 2025, v. 15, n. 3, p. 209, doi. 10.3390/catal15030209

By:

Santos, Rayan P. S.;
Araujo, Lucas L.;
Oliveira Jr., Airton A.;
da Silva, Thamyres F.;
Rocha, Thales G.;
Fernandez-Lafuente, Roberto;
Monteiro, Rodolpho R. C.;
Vieira, Rodrigo S.

Publication type:

Article

Biocatalytic Production of Solketal Esters from Used Oil Utilizing Treated Macauba Epicarp Particles as Lipase Immobilization Support: A Dual Valorization of Wastes for Sustainable Chemistry.

Published in:

Catalysts (2073-4344), 2024, v. 14, n. 10, p. 693, doi. 10.3390/catal14100693

By:

Miguel Júnior, José;
Dimas, João V. B.;
Barbosa, Milson S.;
Gomes, Raphael A. B.;
Carvalho, Ana K. F.;
Soares, Cleide M. F.;
Fernandez-Lafuente, Roberto;
Mendes, Adriano A.

Publication type:

Article

Tuning Almond Lipase Features by Using Different Immobilization Supports.

Published in:

Catalysts (2073-4344), 2024, v. 14, n. 2, p. 115, doi. 10.3390/catal14020115

By:

Cherni, Oumaima;
Carballares, Diego;
Siar, El Hocine;
Abellanas-Perez, Pedro;
de Andrades, Diandra;
Rocha-Martin, Javier;
Bahri, Sellema;
Fernandez-Lafuente, Roberto

Publication type:

Article

The Effects of Buffer Nature on Immobilized Lipase Stability Depend on Enzyme Support Loading.

Published in:

Catalysts (2073-4344), 2024, v. 14, n. 2, p. 105, doi. 10.3390/catal14020105

By:

Abellanas-Perez, Pedro;
Carballares, Diego;
Rocha-Martin, Javier;
Fernandez-Lafuente, Roberto

Publication type:

Article

Multienzymatic Catalysis and Enzyme Co-Immobilization.

Published in:

Catalysts (2073-4344), 2023, v. 13, n. 12, p. 1488, doi. 10.3390/catal13121488

By:

Fernandez-Lafuente, Roberto

Publication type:

Article

The Immobilization and Stabilization of Trypsin from the Porcine Pancreas on Chitosan and Its Catalytic Performance in Protein Hydrolysis.

Published in:

Catalysts (2073-4344), 2023, v. 13, n. 10, p. 1344, doi. 10.3390/catal13101344

By:

Miguez, João Pedro;
Fernandez-Lafuente, Roberto;
Tavano, Olga Luisa;
Mendes, Adriano Aguiar

Publication type:

Article

Technical–Economic Assessment—The Missing Piece for Increasing the Attractiveness of Applied Biocatalysis in Ester Syntheses?

Published in:

Catalysts (2073-4344), 2023, v. 13, n. 2, p. 223, doi. 10.3390/catal13020223

By:

de Sousa, Ronaldo Rodrigues;
de Castro, Rui de Paula Vieira;
Assis, Nadinne Medeiros;
da Silva, Ayla Sant'Ana;
Freire, Denise Maria Guimarães;
Fernandez-Lafuente, Roberto;
Ferreira-Leitão, Viridiana Santana

Publication type:

Article

Synergy of Ion Exchange and Covalent Reaction: Immobilization of Penicillin G Acylase on Heterofunctional Amino-Vinyl Sulfone Agarose.

Published in:

Catalysts (2073-4344), 2023, v. 13, n. 1, p. 151, doi. 10.3390/catal13010151

By:

da Rocha, Thays N.;
Morellon-Sterling, Roberto;
Gonçalves, Luciana R. B.;
Bolivar, Juan M.;
Alcántara, Andrés R.;
Rocha-Martin, Javier;
Fernández-Lafuente, Roberto

Publication type:

Article

Heterofunctional Methacrylate Beads Bearing Octadecyl and Vinyl Sulfone Groups: Tricks to Obtain an Interfacially Activated Lipase from Thermomyces lanuginosus and Covalently Attached to the Support.

Published in:

Catalysts (2073-4344), 2023, v. 13, n. 1, p. 108, doi. 10.3390/catal13010108

By:

Guimarães, José R.;
Carballares, Diego;
Rocha-Martin, Javier;
Alcántara, Andrés R.;
Tardioli, Paulo W.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Co-Enzymes with Dissimilar Stabilities: A Discussion of the Likely Biocatalyst Performance Problems and Some Potential Solutions.

Published in:

Catalysts (2073-4344), 2022, v. 12, n. 12, p. 1570, doi. 10.3390/catal12121570

By:

Høst, Amalie Vang;
Morellon-Sterling, Roberto;
Carballares, Diego;
Woodley, John M.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Mineralization of Lipase from Thermomyces lanuginosus Immobilized on Methacrylate Beads Bearing Octadecyl Groups to Improve Enzyme Features.

Published in:

Catalysts (2073-4344), 2022, v. 12, n. 12, p. 1552, doi. 10.3390/catal12121552

By:

Guimarães, José R.;
Carballares, Diego;
Rocha-Martin, Javier;
Tardioli, Paulo W.;
Fernandez-Lafuente, Roberto

Publication type:

Article

The Stability of Dimeric D -amino Acid Oxidase from Porcine Kidney Strongly Depends on the Buffer Nature and Concentration.

Published in:

Catalysts (2073-4344), 2022, v. 12, n. 9, p. N.PAG, doi. 10.3390/catal12091009

By:

Carballares, Diego;
Rocha-Martin, Javier;
Fernandez-Lafuente, Roberto

Publication type:

Article

Production of Jet Biofuels by Catalytic Hydroprocessing of Esters and Fatty Acids: A Review.

Published in:

Catalysts (2073-4344), 2022, v. 12, n. 2, p. 237, doi. 10.3390/catal12020237

By:

Monteiro, Rodolpho R. C.;
dos Santos, Isabela A.;
Arcanjo, Maria R. A.;
Cavalcante Jr., Célio L.;
de Luna, Francisco M. T.;
Fernandez-Lafuente, Roberto;
Vieira, Rodrigo S.

Publication type:

Article

Performance of Liquid Eversa on Fatty Acid Ethyl Esters Production by Simultaneous Esterification/Transesterification of Low-to-High Acidity Feedstocks.

Published in:

Catalysts (2073-4344), 2021, v. 11, n. 12, p. 1486, doi. 10.3390/catal11121486

By:

Vieira, Ana Carolina;
Cansian, Ana Bárbara Moulin;
Guimarães, José Renato;
Vieira, Angelica Marquettotti Salcedo;
Fernandez-Lafuente, Roberto;
Tardioli, Paulo Waldir

Publication type:

Article

Simplified Method to Optimize Enzymatic Esters Syntheses in Solvent-Free Systems: Validation Using Literature and Experimental Data.

Published in:

Catalysts (2073-4344), 2021, v. 11, n. 11, p. 1357, doi. 10.3390/catal11111357

By:

Sousa, Ronaldo Rodrigues de;
Silva, Ayla Sant'Ana da;
Fernandez-Lafuente, Roberto;
Ferreira-Leitão, Viridiana Santana

Publication type:

Article

Aqueous Extraction of Seed Oil from Mamey Sapote (Pouteria sapota) after Viscozyme L Treatment.

Published in:

Catalysts (2073-4344), 2021, v. 11, n. 6, p. 748, doi. 10.3390/catal11060748

By:

Tacias-Pascacio, Veymar G.;
Rosales-Quintero, Arnulfo;
Rodrigues, Rafael C.;
Castañeda-Valbuena, Daniel;
Díaz-Suarez, Pablo F.;
Torrestiana-Sánchez, Beatriz;
Jiménez-Gómez, Erik F.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Lipase Immobilization in Mesoporous Silica Nanoparticles for Biofuel Production.

Published in:

Catalysts (2073-4344), 2021, v. 11, n. 5, p. 629, doi. 10.3390/catal11050629

By:

Costantini, Aniello;
Califano, Valeria;
Fernandez-Lafuente, Roberto

Publication type:

Article

Immobilization of the Peroxygenase from Agrocybe aegerita. The Effect of the Immobilization pH on the Features of an Ionically Exchanged Dimeric Peroxygenase.

Published in:

Catalysts (2073-4344), 2021, v. 11, n. 5, p. 560, doi. 10.3390/catal11050560

By:

Carballares, Diego;
Morellon-Sterling, Roberto;
Xu, Xiaomin;
Hollmann, Frank;
Fernandez-Lafuente, Roberto;
Topakas, Evangelos

Publication type:

Article

Multi-Combilipases: Co-Immobilizing Lipases with Very Different Stabilities Combining Immobilization via Interfacial Activation and Ion Exchange. The Reuse of the Most Stable Co-Immobilized Enzymes after Inactivation of the Least Stable Ones.

Published in:

Catalysts (2073-4344), 2020, v. 10, n. 10, p. 1207, doi. 10.3390/catal10101207

By:

Arana-Peña, Sara;
Carballares, Diego;
Cortés Corberan, Vicente;
Fernandez-Lafuente, Roberto

Publication type:

Article

Composites of Crosslinked Aggregates of Eversa® Transform and Magnetic Nanoparticles. Performance in the Ethanolysis of Soybean Oil.

Published in:

Catalysts (2073-4344), 2020, v. 10, n. 8, p. 817, doi. 10.3390/catal10080817

By:

Miranda, Letícia Passos;
Guimarães, José Renato;
Giordano, Roberto Campos;
Fernandez-Lafuente, Roberto;
Tardioli, Paulo Waldir

Publication type:

Article

Immobilized Biocatalysts of Eversa® Transform 2.0 and Lipase from Thermomyces Lanuginosus: Comparison of Some Properties and Performance in Biodiesel Production.

Published in:

Catalysts (2073-4344), 2020, v. 10, n. 7, p. 738, doi. 10.3390/catal10070738

By:

Martínez-Sanchez, Javier A.;
Arana-Peña, Sara;
Carballares, Diego;
Yates, Malcom;
Otero, Cristina;
Fernandez-Lafuente, Roberto

Publication type:

Article

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates.

Published in:

Catalysts (2073-4344), 2020, v. 10, n. 6, p. 605, doi. 10.3390/catal10060605

By:

Arana-Peña, Sara;
Carballares, Diego;
Berenguer-Murcia, Ángel;
Alcántara, Andrés R.;
Rodrigues, Rafael C.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Glyoxyl-Activated Agarose as Support for Covalently Link Novo-Pro D: Biocatalysts Performance in the Hydrolysis of Casein.

Published in:

Catalysts (2073-4344), 2020, v. 10, n. 5, p. 466, doi. 10.3390/catal10050466

By:

Lopes, Laiane Antunes;
Novelli, Paula Kern;
Fernandez-Lafuente, Roberto;
Tardioli, Paulo Waldir;
Giordano, Raquel Lima Camargo

Publication type:

Article

Sustainable Enzymatic Synthesis of a Solketal Ester—Process Optimization and Evaluation of Its Antimicrobial Activity.

Published in:

Catalysts (2073-4344), 2020, v. 10, n. 2, p. 218, doi. 10.3390/catal10020218

By:

Mendoza-Ortiz, Patricia A.;
Gama, Rafaela S.;
Gómez, Omar C.;
Luiz, Jaine H. H.;
Fernandez-Lafuente, Roberto;
Cren, Erika C.;
Mendes, Adriano A.

Publication type:

Article

Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications.

Published in:

Catalysts (2073-4344), 2019, v. 9, n. 12, p. 1035, doi. 10.3390/catal9121035

By:

Tacias-Pascacio, Veymar G.;
García-Parra, Esmeralda;
Vela-Gutiérrez, Gilber;
Virgen-Ortiz, Jose J.;
Berenguer-Murcia, Ángel;
Alcántara, Andrés R.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Combi-CLEAs of Glucose Oxidase and Catalase for Conversion of Glucose to Gluconic Acid Eliminating the Hydrogen Peroxide to Maintain Enzyme Activity in a Bubble Column Reactor.

Published in:

Catalysts (2073-4344), 2019, v. 9, n. 8, p. 657, doi. 10.3390/catal9080657

By:

Mafra, Agnes Cristina Oliveira;
Ulrich, Letícia Gazzotto;
Kornecki, Jakub F.;
Fernandez-Lafuente, Roberto;
Tardioli, Paulo Waldir;
Ribeiro, Marcelo Perencin de Arruda

Publication type:

Article

Dextran Aldehyde in Biocatalysis: More Than a Mere Immobilization System.

Published in:

Catalysts (2073-4344), 2019, v. 9, n. 7, p. 622, doi. 10.3390/catal9070622

By:

Tacias-Pascacio, Veymar G.;
Ortiz, Claudia;
Rueda, Nazzoly;
Berenguer-Murcia, Ángel;
Acosta, Niuris;
Aranaz, Inmaculada;
Civera, Concepción;
Fernandez-Lafuente, Roberto;
Alcántara, Andrés R.

Publication type:

Article

Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue.

Published in:

Catalysts (2073-4344), 2019, v. 9, n. 7, p. 576, doi. 10.3390/catal9070576

By:

Rios, Nathalia S.;
Arana-Peña, Sara;
Mendez-Sanchez, Carmen;
Lokha, Yuliya;
Cortes-Corberan, Vicente;
Gonçalves, Luciana R. B.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Reuse of Lipase from Pseudomonas fluorescens via Its Step-by-Step Coimmobilization on Glyoxyl-Octyl Agarose Beads with Least Stable Lipases.

Published in:

Catalysts (2073-4344), 2019, v. 9, n. 5, p. 487, doi. 10.3390/catal9050487

By:

Rios, Nathalia S.;
Arana-Peña, Sara;
Mendez-Sanchez, Carmen;
Ortiz, Claudia;
Gonçalves, Luciana R. B.;
Fernandez-Lafuente, Roberto

Publication type:

Article

Preparation of Crosslinked Enzyme Aggregates of a Thermostable Cyclodextrin Glucosyltransferase from Thermoanaerobacter sp. Critical Effect of the Crosslinking Agent.

Published in:

Catalysts (2073-4344), 2019, v. 9, n. 2, p. 120, doi. 10.3390/catal9020120

By:

Rojas, Mayerlenis Jimenez;
Amaral-Fonseca, Murilo;
Zanin, Gisella Maria;
Fernandez-Lafuente, Roberto;
Giordano, Raquel de Lima Camargo;
Tardioli, Paulo Waldir

Publication type:

Article

Immobilization of Eversa Lipase on Octyl Agarose Beads and Preliminary Characterization of Stability and Activity Features.

Published in:

Catalysts (2073-4344), 2018, v. 8, n. 11, p. 511, doi. 10.3390/catal8110511

By:

Arana-Peña, Sara;
Lokha, Yuliya;
Fernández-Lafuente, Roberto

Publication type:

Article

Preparation of Magnetic Cross-Linked Amyloglucosidase Aggregates: Solving Some Activity Problems.

Published in:

Catalysts (2073-4344), 2018, v. 8, n. 11, p. 496, doi. 10.3390/catal8110496

By:

Amaral-Fonseca, Murilo;
Kopp, Willian;
Giordano, Raquel de Lima Camargo;
Fernández-Lafuente, Roberto;
Tardioli, Paulo Waldir

Publication type:

Article

Maltose Production Using Starch from Cassava Bagasse Catalyzed by Cross-Linked β-Amylase Aggregates.

Published in:

Catalysts (2073-4344), 2018, v. 8, n. 4, p. 170, doi. 10.3390/catal8040170

By:

Araujo-Silva, Rafael;
Mafra, Agnes Cristina Oliveira;
Rojas, Mayerlenis Jimenez;
Kopp, Willian;
Giordano, Roberto De Campos;
Fernandez-Lafuente, Roberto;
Tardioli, Paulo Waldir

Publication type:

Article

Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses.

Published in:

Catalysts (2073-4344), 2018, v. 8, n. 4, p. 149, doi. 10.3390/catal8040149

By:

Siar, El-Hocine;
Arana-Peña, Sara;
Barbosa, Oveimar;
Zidoune, Mohammed Nasreddine;
Fernandez-Lafuente, Roberto

Publication type:

Article

Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β-galactosidase from Aspergillus oryzae.

Published in:

Catalysts (2073-4344), 2017, v. 7, n. 9, p. 250, doi. 10.3390/catal7090250

By:

Zaak, Hadjer;
Peirce, Sara;
de Albuquerque, Tiago L.;
Sassi, Mohamed;
Fernandez-Lafuente, Roberto

Publication type:

Article