Works by Ackerley, David F.

Results: 25

Directed evolution of the B. subtilis nitroreductase YfkO improves activation of the PET-capable probe SN33623 and CB1954 prodrug.

Published in:

Biotechnology Letters, 2021, v. 43, n. 1, p. 203, doi. 10.1007/s10529-020-02992-0

By:

Rich, Michelle H.;
Sharrock, Abigail V.;
Ashoorzadeh, Amir;
Patterson, Adam V.;
Smaill, Jeff B.;
Ackerley, David F.

Publication type:

Article

The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes.

Published in:

Biotechnology Letters, 2020, v. 42, n. 12, p. 2665, doi. 10.1007/s10529-020-02972-4

By:

Brown, Alistair S.;
Calcott, Mark J.;
Collins, Vincent M.;
Owen, Jeremy G.;
Ackerley, David F.

Publication type:

Article

A cofactor consumption screen identifies promising NfsB family nitroreductases for dinitrotoluene remediation.

Published in:

Biotechnology Letters, 2019, v. 41, n. 10, p. 1155, doi. 10.1007/s10529-019-02716-z

By:

Williams, Elsie M.;
Sharrock, Abigail V.;
Rylott, Elizabeth L.;
Bruce, Neil C.;
MacKichan, Joanna K.;
Ackerley, David F.

Publication type:

Article

Evaluation of NfsA-like nitroreductases from <italic>Neisseria meningitidis</italic> and <italic>Bartonella henselae</italic> for enzyme-prodrug therapy, targeted cellular ablation, and dinitrotoluene bioremediation.

Published in:

Biotechnology Letters, 2018, v. 40, n. 2, p. 359, doi. 10.1007/s10529-017-2472-5

By:

Rich, Michelle H.;
Sharrock, Abigail V.;
Hall, Kelsi R.;
Ackerley, David F.;
MacKichan, Joanna K.

Publication type:

Article

Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT.

Published in:

Pharmaceutics, 2021, v. 13, n. 7, p. 1066, doi. 10.3390/pharmaceutics13071066

By:

Brown, Alistair S.;
Owen, Jeremy G.;
Jung, James;
Baker, Edward N.;
Ackerley, David F.

Publication type:

Article

Interrogation of the Structure–Activity Relationship of a Lipophilic Nitroaromatic Prodrug Series Designed for Cancer Gene Therapy Applications.

Published in:

Pharmaceuticals (14248247), 2022, v. 15, n. 2, p. 185, doi. 10.3390/ph15020185

By:

Ashoorzadeh, Amir;
Mowday, Alexandra M.;
Guise, Christopher P.;
Silva, Shevan;
Bull, Matthew R.;
Abbattista, Maria R.;
Copp, Janine N.;
Williams, Elsie M.;
Ackerley, David F.;
Patterson, Adam V.;
Smaill, Jeff B.

Publication type:

Article

Restoring Tumour Selectivity of the Bioreductive Prodrug PR-104 by Developing an Analogue Resistant to Aerobic Metabolism by Human Aldo-Keto Reductase 1C3.

Published in:

Pharmaceuticals (14248247), 2021, v. 14, n. 12, p. 1231, doi. 10.3390/ph14121231

By:

Abbattista, Maria R.;
Ashoorzadeh, Amir;
Guise, Christopher P.;
Mowday, Alexandra M.;
Mittra, Rituparna;
Silva, Shevan;
Hicks, Kevin O.;
Bull, Matthew R.;
Jackson-Patel, Victoria;
Lin, Xiaojing;
Prosser, Gareth A.;
Lambie, Neil K.;
Dachs, Gabi U.;
Ackerley, David F.;
Smaill, Jeff B.;
Patterson, Adam V.

Publication type:

Article

Portability of the thiolation domain in recombinant pyoverdine non-ribosomal peptide synthetases.

Published in:

BMC Microbiology, 2015, v. 15, n. 1, p. 1, doi. 10.1186/s12866-015-0496-3

By:

Calcott, Mark J.;
Ackerley, David F.

Publication type:

Article

Interactions between an anti-sigma protein and two sigma factors that regulate the pyoverdine signaling pathway in Pseudomonas aeruginosa.

Published in:

BMC Microbiology, 2014, v. 14, n. 1, p. 287, doi. 10.1186/s12866-014-0287-2

By:

Edgar, Rebecca J;
Xin Xu;
Shirley, Matt;
Konings, Anna F;
Martin, Lois W;
Ackerley, David F;
Lamont, Iain L

Publication type:

Article

Structural Evaluation of a Nitroreductase Engineered for Improved Activation of the 5-Nitroimidazole PET Probe SN33623.

Published in:

International Journal of Molecular Sciences, 2024, v. 25, n. 12, p. 6593, doi. 10.3390/ijms25126593

By:

Sharrock, Abigail V.;
Mumm, Jeff S.;
Williams, Elsie M.;
Čėnas, Narimantas;
Smaill, Jeff B.;
Patterson, Adam V.;
Ackerley, David F.;
Bagdžiūnas, Gintautas;
Arcus, Vickery L.

Publication type:

Article

The Catalysis Mechanism of E. coli Nitroreductase A, a Candidate for Gene-Directed Prodrug Therapy: Potentiometric and Substrate Specificity Studies.

Published in:

International Journal of Molecular Sciences, 2024, v. 25, n. 8, p. 4413, doi. 10.3390/ijms25084413

By:

Valiauga, Benjaminas;
Bagdžiūnas, Gintautas;
Sharrock, Abigail V.;
Ackerley, David F.;
Čėnas, Narimantas

Publication type:

Article

The Crystal Structure of Engineered Nitroreductase NTR 2.0 and Impact of F70A and F108Y Substitutions on Substrate Specificity.

Published in:

International Journal of Molecular Sciences, 2023, v. 24, n. 7, p. 6633, doi. 10.3390/ijms24076633

By:

Sharrock, Abigail V.;
Mumm, Jeff S.;
Bagdžiūnas, Gintautas;
Čėnas, Narimantas;
Arcus, Vickery L.;
Ackerley, David F.

Publication type:

Article

Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues.

Published in:

eLife, 2020, p. 1, doi. 10.7554/eLife.59081

By:

Hall, Kelsi R.;
Robins, Katherine J.;
Williams, Elsie M.;
Rich, Michelle H.;
Calcott, Mark J.;
Copp, Janine N.;
Little, Rory F.;
Schwörer, Ralf;
Evans, Gary B.;
Patrick, Wayne M.;
Ackerley, David F.

Publication type:

Article

Pseudomonas aeruginosa NfsB and nitro-CBI-DEI - a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy.

Published in:

Molecular Cancer, 2013, v. 12, n. 1, p. 1, doi. 10.1186/1476-4598-12-58

By:

Green, Laura K.;
Syddall, Sophie P.;
Carlin, Kendall M.;
Bell, Glenn D.;
Guise, Christopher P.;
Mowday, Alexandra M.;
Hay, Michael P.;
Smaill, Jeffrey B.;
Patterson, Adam V.;
Ackerley, David F.

Publication type:

Article

Engineering the Escherichia coli Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System.

Published in:

Frontiers in Pharmacology, 2021, v. 12, p. 1, doi. 10.3389/fphar.2021.701456

By:

Sharrock, Abigail V.;
McManaway, Sarah P.;
Rich, Michelle H.;
Mumm, Jeff S.;
Hermans, Ian F.;
Tercel, Moana;
Pruijn, Frederik B.;
Ackerley, David F.

Publication type:

Article

Advancing Clostridia to Clinical Trial: Past Lessons and Recent Progress.

Published in:

Cancers, 2016, v. 8, n. 7, p. 63, doi. 10.3390/cancers8070063

By:

Mowday, Alexandra M.;
Guise, Christopher P.;
Ackerley, David F.;
Minton, Nigel P.;
Lambin, Philippe;
Dubois, Ludwig J.;
Theys, Jan;
Smaill, Jeff B.;
Patterson, Adam V.

Publication type:

Article

The Flavin Reductase MsuE Is a Novel Nitroreductase that Can Efficiently Activate Two Promising Next-Generation Prodrugs for Gene-Directed Enzyme Prodrug Therapy.

Published in:

Cancers, 2013, v. 5, n. 3, p. 985, doi. 10.3390/cancers5030985

By:

Green, Laura K.;
Storey, Mathew A.;
Williams, Elsie M.;
Patterson, Adam V.;
Smaill, Jeff B.;
Copp, Janine N.;
Ackerley, David F.

Publication type:

Article

Enzyme improvement in the absence of structural knowledge: a novel statistical approach.

Published in:

ISME Journal: Multidisciplinary Journal of Microbial Ecology, 2008, v. 2, n. 2, p. 171, doi. 10.1038/ismej.2007.100

By:

Barak, Yoram;
Nov, Yuval;
Ackerley, David F.;
Matin, A.

Publication type:

Article

Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa.

Published in:

Molecular Microbiology, 2017, v. 106, n. 6, p. 891, doi. 10.1111/mmi.13855

By:

Edgar, Rebecca J.;
Hampton, Georgina E.;
Garcia, G. Patricia Casas;
Maher, Megan J.;
Perugini, Matthew A.;
Ackerley, David F.;
Lamont, Iain L.

Publication type:

Article

Nitroreductase gene-directed enzyme prodrug therapy: insights and advances toward clinical utility.

Published in:

Biochemical Journal, 2015, v. 471, n. 2, p. 131, doi. 10.1042/BJ20150650

By:

Williams, Elsie M.;
Little, Rory F.;
Mowday, Alexandra M.;
Rich, Michelle H.;
Chan-Hyams, Jasmine V.E.;
Copp, Janine N.;
Smaill, Jeff B.;
Patterson, Adam V.;
Ackerley, David F.

Publication type:

Article

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution.

Published in:

Nature Communications, 2020, v. 11, n. 1, p. N.PAG, doi. 10.1038/s41467-020-18365-0

By:

Calcott, Mark J.;
Owen, Jeremy G.;
Ackerley, David F.

Publication type:

Article

<i>Escherichia coli</i> NemA Is an Efficient Chromate Reductase That Can Be Biologically Immobilized to Provide a Cell Free System for Remediation of Hexavalent Chromium.

Published in:

PLoS ONE, 2013, v. 8, n. 3, p. 1, doi. 10.1371/journal.pone.0059200

By:

Robins, Katherine J.;
Hooks, David O.;
Rehm, Bernd H. A.;
Ackerley, David F.

Publication type:

Article

High-Throughput Screening for Inhibitors of the SARS-CoV-2 Protease Using a FRET-Biosensor.

Published in:

Molecules, 2020, v. 25, n. 20, p. 4666, doi. 10.3390/molecules25204666

By:

Brown, Alistair S.;
Ackerley, David F.;
Calcott, Mark J.

Publication type:

Article

Mechanism of Two-/Four-Electron Reduction of Nitroaromatics by Oxygen-Insensitive Nitroreductases: The Role of a Non-Enzymatic Reduction Step.

Published in:

Molecules, 2018, v. 23, n. 7, p. 1672, doi. 10.3390/molecules23071672

By:

Valiauga, Benjaminas;
Misevičienė, Lina;
Rich, Michelle H.;
Ackerley, David F.;
Šarlauskas, Jonas;
Čėnas, Narimantas

Publication type:

Article

A sensitive single-enzyme assay system using the non-ribosomal peptide synthetase BpsA for measurement of L-glutamine in biological samples.

Published in:

Scientific Reports, 2017, p. 41745, doi. 10.1038/srep41745

By:

Brown, Alistair S.;
Robins, Katherine J.;
Ackerley, David F.

Publication type:

Article