Antioxidative Effect of Naringenin on the Activity of Superoxide Dismutase and Glutathione Peroxidase in HepG2 Cells under Hyperglycaemic Conditions.

Kneževiæ, T.; Petlevski, R.

Background: Oxidative stress is believed to play a pivotal role in the etiology and pathogenesis of diabetes mellitus and its late complications. Hyperglycaemia- induced oxidative stress in diabetes is a consequence of both increased production of free radicals and reduced capacity of antioxidative defence. The citrus-derived flavonoid, naringenin, possesses antioxidative, free radical scavenging and metal chelating properties. The aim of this work was to investigate the antioxidative effect of naringenin on the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in HepG2 cells under hyperglycaemic conditions. Materials and methods: Activities of SOD and GPx were measured in lysates of HepG2 cells (ATCC HB8065, Manassas, USA). HepG2 cells tested were divided in six groups: control group, diabetes group (30 mM glucose) and four diabetes-naringenin groups (30 mM glucose naringenin in one of the following concentrations: 1000, 100, 10 and 1 μg/mL). Activities of both enzymes were measured spectrophotometrically and expressed by protein concentrations. Results: The results were reported as specific activities of SOD and GPx. All groups tested were compared to diabetes group. Student t-test was used to compare tested groups. P-value <0.05 was adopted as statistically significant. Diabetes-naringenin group (30 mM glucose 1 μg/mL naringenin) showed significantly increased activities of SOD (P<0.001) and GPx (P=0.007). On the other hand, in diabetes-naringenin group (30mM glucose 10 μg/mL naringenin) only SOD showed increased activity (P=0.002). The other two diabetesnaringenin groups did not show significantly higher activities of SOD and GPx. Conclusion: The results obtained from this work indicate that naringenin exhibits its antioxidative effect on the activity of SOD and GPx in HepG2 cells under hyperglycaemic conditions when applied in lower concentrations, 1 μg/mL and 10 μg/ mL.

ANTIOXIDANTS; NARINGENIN; SUPEROXIDE dismutase; GLUTATHIONE peroxidase; GLUCAGON; CONTROL groups; T-test (Statistics)

Biochemia Medica, 2014, Vol 24, pS64

1330-0962

Academic Journal