An animal experiment study on the effect of periodontitis on atherosclerosis.

Wang Zhou; Zhang Mingzhu; Yu Zhuo; Shui Yanqing; Ding Yun; Lei Yayan

Objective To make an animal periodontitis and atherosclerosis compound model, and to study the effects of periodontitis on atherosclerosis. Methods 36 Japan rabbits were randomly divided into four groups: Including perio-dontitis model group, periodontitis and atherosclerosis compound model group, atherosclerosis model group and control group. Periodontitis model was initiated by ligating floss around teeth cervical and oral inoculating with Porphyromonas gingivalis (P.gingivalis). Atherosclerosis was established by single iliac artery of balloon-injured rabbit. Histopathological change of injured iliac artery was observed under optical microscope after hematoxylin-eosion stain. Elastica van Gieson-stained sections were used for the morphometric analysis. We measured intimai and medial lesion areas in iliac artery cross-sections as well as the intimal/medial ratio (I/M). We also analyzed P.gingivalis 16S rDNA amplification with nested-polymerase chain reaction (nested-PCR), and detect systemic proinflammatory mediators with enzyme linked im-munosorbent assayOELISA), including C-reactive protein(CRP), interleukin-6(IL-6) and interleukin-1β (IL-1β). Results The serum levels of CRP, IL-6 and IL-lß increased obviously among the compound model group than other groups (P<0.01). Histopathological observation revealed the compound model group in I/M was bigger than other groups (P< 0.01). P.gingivalis 16S rDNA was detected among the periodontitis model group and the compound model group by nested-PCR. Conclusion Periodontitis may accelerate intimai hyperplasia in balloon-injured iliac arteries by upgrade of systemic inflammation factors and local bacterial infection.

West China Journal of Stomatology, 2012, Vol 30, Issue 3, p308

1000-1182

Academic Journal

10.3969/j.issn.1000-1182.2012.03.021