Evaluation of the Potential Effect of Ellagic Acid on Cotinine and Antioxidant Levels in Nicotine-Induced Rats.

Mazen M. Jamil Al-Obaidi; Fouad Hussain Al-Bayaty; Rami Al-Batran; Mahmood Ameen Abdulla; Pouya Hassandarvish; Farha Ariffin; Jamal Hussain

Background: Ellagic Acid (EA) has been receiving the most attention because it has potent antioxidant activity, radical scavenging capacity, chemopreventive and antiapoptotic properties. Objective: The purpose of this study was to evaluate, the effect of EA on cotinine and antioxidants levels in nicotinic induced rats. Twenty-four adult male Sprague-Dawley (SD) rats were divided into four groups; Group (1) control normal received 0.9% NaCl. Group (2) control injected with Nicotine (Ni) subcutaneously (3 mg/kg) for 4 weeks to convert the animals into smoker. Groups (3) and (4) (Experimental groups) were injected also subcutaneously with Ni for 4 weeks, then, were administered orally with EA (50 mg/kg) (100 mg/kg) respectively for 30 days using gastric tube. Then, whole animals were sacrificed. The effects of EA on endogenous enzymes Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CTA), and Malondialdehyde (MDA), Nitric oxide (NO) and cotinine levels (ELISA Technique) were performed in rats. Results: nicotinic rats treated with EA significantly induced an increase in endogenous enzymes, and decreased in MDA, NO level compared the control group and Ni induced rats. Nicotinic rats treated with EA significantly depleted the cotinine level. Acute and sub-chronic toxicity studies revealed the non-toxic nature of EA. Conclusions: The results of the present study showed that EA significantly increased the endogenous enzymes and decrease the MDA, NO and cotinine level may possibly due to antioxidant content of EA in nicotinic rats. EA may become a promising candidate for the treatment of oxidative stress. It can be used for nicotine reduction in smokers.

ANALYSIS of variance; ANIMAL experimentation; ANTIOXIDANTS; BENZOPYRANS; ENZYME-linked immunosorbent assay; NICOTINE; NITRIC oxide; RATS; RESEARCH funding; STATISTICS; MALONDIALDEHYDE; DATA analysis; OXIDATIVE stress; COTININE; DESCRIPTIVE statistics

International Medical Journal, 2014, Vol 21, Issue 2, p150

1341-2051

Academic Journal