HEPATOPROTECTIVE EFFECT OF EMBILICA OFFICINALIS AND ITS SILVER NANOPARTICLES AGAINST CCl4 INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS.

BHUVANESWARI, R.; CHIDAMBARANATHAN, N.; JEGATHEESAN, K.

The present study was an attempt to investigate the hepatoprotective and antioxidant properties of aqueous fruit extract of Embilica officinalis (E.O) and silver nanoparticles synthesized using Embilica officinalis (E.O) fruit extract in wistar male albino rats. The rats were divided into eight groups with six animals each. Group I control rats received normal feed. Group II rats were treated with hepatotoxic agent CCl4 (2 ml/kg/day). Group III was given E.O (300 mg/kg) aqueous fruit extract. Group IV was treated with E.O (300 mg/kg) aqueous fruit extract and CCl4 (2 ml/kg/day). Group V rats received E.O silver nanoparticles and CCl4 (2 ml/kg/day). Group VI was treated with Silymarin (100 mg/kg). Group VII received Silymarin (100 mg/kg) along with CCl4 (2ml/kg/day).Group VIII rats were treated with Silymarin silver nanoparticles and CCl4 (2 ml/kg/day). The substantially elevated enzymatic levels of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline Phosphatase (ALP), Lactate dehydrogenase (LDH) and Bilirubin by CCl4 were restored toward normal when treated with aqueous fruit extract of Embilica officinalis. The total protein level has also been increased toward the normal value. Silymarin was used as standard reference and it has significant hepatoprotective effect against the CCl4 induced hepatotoxicity in rats. The E.O and Silymarin silver nanoparticles also significantly restored the enzymes level to normal. The biochemical observation along with histopathological examination shows that they have the antioxidant and hepatoprotective activity. The fruit extract of E.O, Silymarin and its silver nanoparticles could be effective antioxidants and has hepatoprotective activity.

CARBON tetrachloride; CARBON compounds; PRECIOUS metals; SILYMARIN; NANOSTRUCTURED materials synthesis; SILVER nanoparticles; SILVER nanoparticles spectra

Digest Journal of Nanomaterials & Biostructures (DJNB), 2014, Vol 9, Issue 1, p223

1842-3582

Academic Journal