Selection of Korean Wheat Geirmplasms with Low Polyphenol Oxidase using Polyphenol Oxidase-Specific DNA Markers.

Kycong-Hoon Kim; Chon-Sik Kang; Young-Keun Cheong; Chul Soo Paik; Kyung-Ho Kim; Hak-Shin Kim; Young-Jin Kim; Song Joong Yun

The color of wheat flour and dough affect significantly on the quality of wheat. The major factor causing darkening of dough color is activity of polyphenol oxidase (PPO). Therefore, we examined the relationship between the PPO activities and the genotypes of the PPO-specific molecular markers in Korean wheat cultivars, and tested the usefulness of the selected markers for the selection of Korean wheat germplasm lines low in PPO activity. Five sequence-tagged site (STS) markers specific to wheat PPO genes. PPO-05, PPO-16, PPO-18, PPO-30 and PPO-43, were examined for their levels of polymorphism in Korean wheat cultivars. The genotypes of the marker PPO-18, which is linked to Ppo-AI locus, were correlated significantly highly with the levels of grain PPO activity. Furthermore. Korean wheat cultivars could be classified into the high or low PPO activity group by the allele type of Ppo-AI which was differentiated by the PPO-18. Grain PPO activity was lower about twice in the cultivars with Ppo-AI h allele than in those with Ppo-AI a allele. The cultivars with Ppo-Alb allele had lower grain weight and wide grain width, also they had less ash, protein, Fe and total polyphenol than those with Ppo-Ala allele. Thus, the lightness of flour and dough sheet was brighter in the cultivars with Ppo-Alb allele. The four wheal cultivars including Suwon252, which had lowest PPO activity and Ppo-Alb allele, were selected from 200 genetic lines as valuable genetic resources for the breeding of wheat variety with bright white-colored flour.

POLYPHENOL oxidase; GENETIC markers in plants; WHEAT genetics; PLANT germplasm; GENETIC polymorphisms

Korean Journal of Breeding Science, 2012, Vol 44, Issue 1, p11

0250-3360

Academic Journal