Determination of Cyanidin-3-Glucoside Content using Visible/Near Infrared Reflectance Spectroscopy (VIS/NIRS) in Black Rice.

Jeom-Sig Lee; Do-Yeon Kwak; No-Bong Park; You-Chun Song; Sea-Kwan Oh; Jeong-Heui Lee; Mi-Ra Yoon; Hye-Won Kim; Jieun Kwak; Chung-Kon Kim; Young-Sun Hwang; Myoung-Gun Choung

Brown rice grain pigments of black rice have a higher content of bioactive substances such as anti-mutagenic substance than the non-pigmented rice grain. The major anthocyanin pigment contained in black rice was cyanidin-3-glucoside. This study was conducted to establish a rapid analysis method for determining cyanidin-3-glucoside contents in flour and whole rice seeds of black rice using VIS/NIRS technique. A total of 60 black rice samples were used for VIS/NIRS equation model development and validation. The value of coefficient of determination of external validation (r²) and standard error of performance (SEP) in whole rice seed sample were 0.653 and 97.2, respectively. Therefore, the value of it seemed to be difficult to analyze cyanidin-3-glucoside content in whole rice seed samples using VIS/NIRS. However, in rice flour sample, the best accurate equation model was obtained from the partial least square regression (PLS) method. The value of r², SEP and bias were 22.5, 0.922 and -1.45 in the calibration transformed to the N-point smooth of log 1/R signal, 5 factors, respectively. Therefore, the results of our study clearly demonstrate that the VIS/NIRS method would be applicable only for rapid determination of cyanidin-3-glucose content in black rice flour samples.

CYANIDIN; GLUCOSIDES; NEAR infrared reflectance spectroscopy; BROWN rice; ANTHOCYANINS; HIGH performance liquid chromatography

Korean Journal of Breeding Science, 2012, Vol 44, Issue 4, p444

0250-3360

Academic Journal

10.9787/KJBS.2012.44.4.444