IN VITRO PROTECTIVE EFFECTS OF OLIVE POMACE POLAR LIPIDS TOWARDS PLATELET ACTIVATING FACTOR METABOLISM IN HUMAN RENAL CELLS.

Tsoupras, A. B.; Fragopoulou, E.; Iatrou, C.; Demopoulos, C. A.

Platelet-Activating-Factor (PAF) is a potent inflammatory mediator implicated in several pathological situations including atherosclerosis, cardiovascular and renal diseases. Mediterranean Diet contains foods that exhibit protective effects against these pathologies. We have previously described that micro-constituents existing in polar-lipids of olive oil and olive-pomace, with anti-inflammatory effects towards PAF-activity, exhibit also in vivo anti-atherosclerotic activities. In this study we have further examined the in vitro effects of Olive-Pomace-Polar-Lipids (OPPL) towards PAF-metabolism in renal cells. We have evaluated the specific activities of PAFbasic biosynthetic enzymes, PAF-Cholinephosphotransferase (PAF-CPT) and Lyso-PAF-Acetyltransferase (Lyso-PAF-AT), and that of PAF-Acetylhydrolase (PAF-AH) of PAF-degradation in mesangial cells cultured for 24h in the presence of OPPL or not (Control). We have found for the first time that the presence of OPPL in cultured mesangial cells resulted in significant decrease of 31.01±11.32% (p=0.022) of PAF-CPT and activation of 25.83±12.04% (p=0.043) of PAF-AH in these cells. On the other hand, Lyso-PAF-AT remained relative stable after this incubation. The OPPL seem to beneficially induce a reset of PAF-metabolism in renal cells towards the direction of lessening PAF-activity and its sub-sequent pro-inflammatory manifestations, by a simultaneously PAF-biosynthesis reduction and a PAF-degradation increase. However, more tests are needed to fully evaluate the effects of micro-constituents of the Mediterranean Diet towards PAF-metabolism.

PLATELET activating factor; RENAL cell carcinoma; CHOLINEPHOSPHOTRANSFERASE; BIOSYNTHESIS; MEDITERRANEAN diet

Current Topics in Nutraceutical Research, 2011, Vol 9, Issue 3, p105

1540-7535

Academic Journal