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Title: Quick Extraction and Analysis of Gossypol in Cotton Leaves.
Authors: Jing Wei-Wen; Ti Xiao-Nan; Chen Yan-Qin; Zhang Kun-Chi; Liu Xi
Abstract: The terpenoids in cotton contain a unique group of secondary metabolites, which include desoxyhemigossypol (DHG), hemigossypolone (HGQ), hemigossypol (HG), gossypol(G), and heliocides H1, H2, H3 and H4 ( H1-4 ). These compounds have been shown to be important in protecting the plant from cotton insects. An HPLC method has been utilized for separating individual terpenoid and quantitating gossypol in cotton leaves. Some influencing factors, such as extracting and pre-treating process, were discussed and a set of simple, rapid method was developed. At the same time, the result of the UV-visible spectrophotometry (aniline method) was described and compared. The results showed that HPLC analysis was more reasonable for the insect-resistance investigation in cotton.
Subjects: COTTON; LEAVES; GOSSYPOL; EXTRACTION (Chemistry); PLANT metabolites; TERPENES; SPECTROPHOTOMETRY; PLANT resistance to insects
Publication: Natural Product Research & Development, 2012, Vol 24, Issue 4, p498
ISSN: 1001-6880
Publication type: Academic Journal

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Quick Extraction and Analysis of Gossypol in Cotton Leaves.