Title

Determination of Chlorogenic Acid of Different Varieties of Helianthus tuberosus Leaves from Different Areas by HPLC.

Authors

Zhang Hai-juan; Huang Zeng-rong; Long Xiao-hua; Liu Ling; Liu Zhao-pu

Abstract

The aim of this study is to develop a method for determining the content of chlorogenic acid in 3 varieties of Helianthus tuberosus in Dafeng area and 2 varieties of Helianthus tuberosus in Daqing area. Accordingly, HPLC technology was used to determine the chlorogenic acid content in the leaves of the Helianthus tuberosus in different varieties from different areas. The results showed : 1) The calibration curve showed a good linear relationship in the range of 0.01-0.1 mg/mL for chlorogenic acid under the conditions of 1% water-phosphoric-ACE (87:13) as the mobile phase, a flow rate of 1.0 mL/min and detection wavelength of 327 nm. The precision was fine, and the average recovery rate of chorogenic acid was 98.21%, RSD was 0.68%. 2) In Dafeng area, the chloregenic acid contents in the leaves of the dry weight of Nanyu No. 1, Qingyu No. 2, Nanyu No. 10 were 0. 431%, 0.040%, 0.933%. 3) In Daqing area, the chlorogenic acid contents in the leaves of the dry weight of Nanyu No. 1, Nanyu No. 9 were 1.245%, 2.139%. These results suggested that chlorogenic acid content in Helianthus tuberosus leaves was dependent on the variety differences and ecological region differences. This method of HPLC is simple, rapid and accurate and thus is suitable for measuring the chlorogenic acid content in Helianthus tuberosus leaves.

Subjects

CHLOROGENIC acid; JERUSALEM artichoke; CULTIVARS; HIGH performance liquid chromatography; WAVELENGTHS; CHROMATOGRAPHIC analysis

Publication

Natural Product Research & Development, 2011, Vol 23, Issue 6, p1107

ISSN

1001-6880

Publication type

Academic Journal