Title

Determination of Ursolic Acid and Oleanolic Acid in Sambucus adnata Wall by I-IPLC-DAD.

Authors

Luo Zhi-min; Navarro, Elisa Linares; Tao Yah-duo; Shao Yun; Mei Li-juan

Abstract

The study aimed to determine the content of ursolic acid and oleanolic acid in Sambucus adnata Wall using HPLC-DAD. By this method, fusion-RP C18 (4.6 mm x 250 mm,4 µm) was used with methanol-O.2% phosphate in water (90:10) as the mobile phase and UV detection was at 210 rim. In addition, five different extraction methods including microwave-assisted extraction, reflexing extraction, soxhlet extraction, soaking extraction, and ultrasonic extraction were used for determine the content of uraolic acid and oleanolie acid in different parts of S. adnata Wall and the results were compared. Within the injection volume range between 3.6 and 8.4 o-g for ursolic acid and between 3.2 and 16 µg for oleanolic acid, the results had good linearity. The average recovery, was 98.3% (RSD = 1.13%, n = 5) for ursolic acid and 101.4% (n = 5) (RSD = 0.72%, n = 5) for oleanolic acid. The highest content of ursolic acid and oleanolic acid was obtained by soxhlet extraction methods comparing with other methods. In addition, the flower of S. adnata Wall had the highest content of ursolic acid and oleanolic acid and the root had the lowest amount. Conclusively, the HPLCDAD is a stable and credible method for determining the content of ursolic acid and oleanolic acid in S. adnata Wall with satisfactory baseline separation for these two kinds of acids.

Subjects

URSOLIC acid; SAPOGENINS; ELDERS (Plants); HIGH performance liquid chromatography; ULTRAVIOLET detectors; METHANOL; MICROWAVES; EXTRACTION techniques

Publication

Natural Product Research & Development, 2011, Vol 23, Issue 6, p1095

ISSN

1001-6880

Publication type

Academic Journal