Your institution may have access to this item. Find your institution then sign in to continue.

Title: Effects of Conjugated Linoleic Acid Isomers on Gene Expression of Proteins Involved in Lipid Metabolism.
Authors: LI Jie-mei; XU Juan-juan; YANG De-po; CHEN Jia-shu
Abstract: RT-PCR was employed to determine isomer-specific effects of two conjugated linoleic acid (CLA) isomers on enzymes involved in lipid metabolism in cultured 3T3-L1 adipocytes. Concentration of either isomer was 75.4 µmol/L and treatment period was 8 days. mRNA levels of proteins involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis were identified. The results showed that gene expression of UCP1, UCP3, Perilipin and PPAR increased whereas UCP2 (P <0.01) reduced for both CLA isomers. And compared with treatment of cis-9, trans-11 CLA, trans-10, cis-12 CLA significantly up-regulated mRNA levels of PKA (P<0.05), CPT-1 and TNF-α P<0.01). Compared with control, both isomers did not significantly influence gene expression of HSL, ATGL, ACO and Leptin.
Subjects: LINOLEIC acid; GENE expression; LIPID metabolism; FAT cells; APOPTOSIS; TUMOR necrosis factors; POLYMERASE chain reaction
Publication: Natural Product Research & Development, 2011, Vol 23, Issue 4, p704
ISSN: 1001-6880
Publication type: Academic Journal

We found a match

Effects of Conjugated Linoleic Acid Isomers on Gene Expression of Proteins Involved in Lipid Metabolism.