Effects of Tutin and Andrographolide Derivatives on Antioxidant Enzymes in the Oriental Armyworm, Pseudalatia separata (Lep.: Noctuidae).

Cui, Y.; Li, M.; Nan, X.; Li, F.

Effects of tutin and four andrographolide derivatives; (15-chlorobenzylidene-14-Deoxy-11, 12-didehydroandrographolide) (A2); (14-deoxy-11, 12-didehydroandrographolide) (A4b); (15-fluorobenzylidene-14-Deoxy-11, 12-didehydroandrographolide) (A4d) and 15-Bromoacylbenzylidene-14-deoxy-11, 12-didehydroandrographolide (A4e) on the physiological metabolism of the oriental armyworm, Pseudalatia separata (Lep.: Noctuidae) were investigated. Enzyme activities of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) on the 3rd instar larvae of P. separata, fed on leaf discs treated with tutin and the four andrographolide derivatives were determined by colorimetric method. Results indicated that the compounds tutin, A2, A4b, A4d and A4e inhibited the activities of POD and CAT but promoted the SOD activity in the tested larvae. Whereas the inhibition effect of A4b on CAT was slight and the stimulation effect of A4e on SOD was indistinctive. Thus, tutin, A2, A4b, A4d and A4e inhibited the effects of detoxifcation metabolism on oxygen free radicals and H2O2 by interfering with POD, CAT and SOD activities of the tested larvae. A2 and A4d are the optimal compounds to inhibit POD, CAT activities and to promote the SOD activity, compared with the other two andrographolide derivatives and tutin.

ANTIOXIDANTS; ENZYMES; PROTEINS; SUPEROXIDE dismutase; ARMYWORMS; NOCTUIDAE

Egyptian Journal of Biological Pest Control, 2011, Vol 21, Issue 1, p75

1110-1768

Academic Journal