Title

IDENTIFICATION AND QUANTITATIVE DETERMINATION OF HYPERFORIN IN SOME HYPERICUM SPECIES.

Authors

Gîtea, Daniela; Vlase, Laurian; Tămaş, Mircea; Oniga, Ilioara

Abstract

Using HPLC/MS, hyperforin was identified and quantitatively determined in four species of Hypericum (St John's-wort, Johanniskraut) gathered from the wild flora of Bihor County: H. perforatum L., H. maculatum Crantz subsp. typicum Frohl., H. tetrapterum Fries and H. hirsutum L. The identification was carried out by MS detection with standard hyperforin (Sigma-Aldrich), and quantitative determination by calibration curve in hyperforin, in negative ionisation. Hyperforin content was determined as 7.89% in H. perforatum, 0.077% in H. maculatum, 0.103% in H. tetrapterum and 0.67% in H. hirsutum. Considering that hyperforin is one of the most important active principles of Hyperici herba, and its very low content in the other species (from 100 to 10 times lower), the sole utilization of H. perforatum in phytotherapy may be justified.

Subjects

BIHOR Mountains (Romania); ROMANIA; HYPERICUM; IONIZATION (Atomic physics); MEDICAL botany

Publication

Contributii Botanice, 2010, Vol 45, p35

ISSN

0069-9616

Publication type

Academic Journal