Development and validation of a RP-HPLC method for quantification of rottlerin in Kamala (Mallotus philppinensis).

Vishal R., Patel; Madhavi G., Patel; Rakesh K., Patel

Kamala is orange red powder consisting the glands and hair covering the fruit of Mallotus philppinensis Muell. (Euphorbiaceae), a small tree widely distributed throughout India up to an attitude of 1500 m. It is indigenous to southeastern Asia and find wild throughout tropical Asia, Australia and Philippines. In the indigenous system of medicine, Kamala is used as anthelminitic and cathartic. Kamala powder is used in external application for parasitic affection of the skin and also an aphrodisiac, lithotropic, and stypic. Kamala powder is also used as an antiseptic in blisters in the ears. The seed and fruit powder are generally used for urinary and menstrual disorders. A selective, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the quantification of Rottlerin in kamala. The use of reverse phase C18 column using gradient mobile phase of acetonitrile and water enabled the efficient separation of the chemical markers within a 22 minute analysis. Validation of method performs in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery study. The proposed RP-HPLC method was found to be simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.

INDIA; ASIA; KAMALA; EUPHORBIACEAE; LIQUID chromatography; TRADITIONAL medicine; LAXATIVES; PARASITE antigens; APHRODISIACS; ACETONITRILE

Drug Invention Today, 2009, Vol 1, Issue 2, p116

0975-7619

Academic Journal