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Title: Effects of Conjugated Linoleic Acid on the Proliferation and Differentiation of Preadipocyte 3T3-L1.
Authors: LIU Hua; CAO Yu-sheng
Abstract: 3T3-L1 preadipocytes proliferation was detected by MTT method. Accumulation of lipid in the cytoplasm of differentiated adipocytes was observed by oil red O staining. The expression of peroxisome proliferators activated receptor γ2 (PPARγ2) mRNA was detected by RT-PCR. The t10, c12-CLA and two CLA mixture can significantly inhibit 3T3-L1 cell proliferation(P <0.01). Oil red staining showed t10, c12-CLA has strong inhibiting effect on 3T3-L1 cell line differentiation( P < 0.05). RT-PCR showed that 100 µmol/L t10,c12-CLA and 50 µmol./L t10,c12-CLA significantly reduced the expression of PPARγ2 mRNA as 52.1% and 83.0% in comparison with control group respectively.
Subjects: LINOLEIC acid; CELL proliferation; CELL differentiation; FAT cells; PEROXISOMES; MESSENGER RNA; REVERSE transcriptase; POLYMERASE chain reaction
Publication: Natural Product Research & Development, 2008, Vol 20, Issue 1, p8
ISSN: 1001-6880
Publication type: Academic Journal

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Effects of Conjugated Linoleic Acid on the Proliferation and Differentiation of Preadipocyte 3T3-L1.