183: Decursin affects Wnt/beta catenin pathway on prostate cancer cell lines.

Jun, Choi Ik; Sup, Kwak Ho; Ho, Yoon Jang; Don, Lee Sang; Wan, Lee; Min, Chung Jae; Hyo, Kim Tae; Dong II, Kang

Introduction: Decursin is a pyranocurmarin found in the roots of Angelica gigas Nakai, which has been traditionally used to treat anemia. Decursin has cytotoxic effect on leukemia cells and prostatic carcinoma cells. We carried out this study to investigate the relationship of Wnt/β-catenin pathway and decursin in prostate cancer cells. Methods: PC-3, LNCaP cell lines were used in this study. Cell death assays and cell apoptosis analysis by FACScan was done. β-catenin protein was measured by western blot analysis after treatment with decursin. Real time polymerase chain reaction (RT-PCR) was performed to detect MMP-7 mRNA expression. Results: Decursin increase cell death and apoptosis (0.5uM to 100uM) in PC-3 and LNCaP cells. Decursin (0.5- 100uM) showed dose and time dependent increase of cell death and apoptosis for 24, 48, 72 hours. Expression of β-catenin protein decrease by dose depenent in PC-3 and LNCaP cell. In both PC-3 and LNCaP cells, mRNA expressions of MMP-7 decreased at 6, 24 hours by dose-dependently compared to untreated cells. Conclusions: Decursin affects viability of prostate cancer cells, and cancer cell death was associated with an increase in apoptosis. This study suggests that Decursin can regulate protein of Wnt/β-catenin pathway in prostate cancer cell.

COUMARINS; WNT proteins; CELL lines; PROSTATE cancer; CANCER cells; CELL death; APOPTOSIS

Indian Journal of Urology, 2008, Vol 24, pS114

0970-1591

Academic Journal