Title

Reciprocal induction of dipeptide and glucose co-transporters after small bowel transplantation.

Authors

Nakai, Koji; Hamada, Yoshinori; Kitagawa, Katsuhiko; Baden, Tetsuya; Yonekura, Yasuhiro; Tanano, Akihide; Okumura, Tadayoshi

Abstract

[Aims] Small bowel transplantation is one of the most curative treatments for patients of short bowel syndrome, where the rapid recovery and maintenance of graft function are crucial. Dipeptide transporter (PEPT1) as well as amino acid transporter plays an important role for transport of nitrogen source from apical side to intracellular matrix on entcrocytes. However, the mechanisms of PEPT1 induction are obscure. We investigated how small bowel transplantation affects the induction of PEPTI on rats. [Materials & Methods] The jejunum graft (30-cm) with aorta and portal vein was removed from the donor (BN rats) and small bowel transplantation was performed in the recipient (Low rats) with Thiry-Vella loop. On postoperative day (POD) 7, the stumps of graft were removed and interposed into a same part of 30-cm recipient jejunum. All animals were received FK-506, 0.5 mg/kg everyday. Samples for Western blot analysis and morphologic studies were isolated at POD 7 (n=3) and 14 (n=3). [Results] Histological analysis revealed flint there was no significant difference between POD 7 and 14 oft villus height and crypt depth. Western blot analysis revealed that induction of PEPT1 increased at POD 7 as compared with levels of normal rats and then decreased at POD 14. In contrast, induction of SGLT1 was decreased at POD 7, and then increased at POD 14. [Conclusions] These results suggest that there is a different regulation in the induction of PEPT1 and SGLT1 after small bowel transplantation on rats. It seems that intestinal transplantation may be affected according to the dietary passage and neccessary elements in recipients.

Subjects

SMALL intestine; PEPTIDES; AMINO acids; LABORATORY rats; POSTOPERATIVE period; TRANSPLANTATION of organs, tissues, etc.

Publication

Asia Pacific Journal of Clinical Nutrition, 2002, Vol 11, pS59

ISSN

0964-7058

Publication type

Academic Journal