ENDOTHELIAL NITRIC OXIDE SYNTHASE, ANGIOTENSINOGEN AND ALDOSTERONE SYNTHASE GENE POLYMORPHISMS IN TYPE 2 DIABETES MELLITUS AND THEIR ASSOCIATION WITH ARTERIAL STIFFNESS.

Zafar, S.; Rehman, A.; Rahman, A. R. A.

Introduction: The objective of the present study was to determine the prevalence of endothelial nitric oxide synthase (eNOS) G894T, angiotensinogen (AGT) M235T and aldosterone synthase (CYP11B2) T344C polymorphisms in type 2 diabetes mellitus (T2 DM) and their association with arterial stiffness. Method: The study involved 194 T2 DM and 190 control subjects. Arterial stiffness was measured as aortic augmentation index (AIx) by Sphygmocor PWA system. The genotyping was done with previously described PCR-RFLP methods. Allele frequencies were determined and association between AIx and polymorphisms among different populations was determined by ANCOVA. Results: There were no significant difference between genotype distribution of G894T, M235T and T344C polymorphisms between T2 DM and control subjects. The G894T polymorphism did not influence the blood pressure (BP) in both groups but it was significantly associated with increased AIx in diabetic patients (p=0.020). The T344C polymorphism was significantly associated with SBP (p=0.009) and DBP (p=0.059) in diabetic patients only while no association was found with AIx in both groups. The M235T polymorphism was not associated with BP and AIx in T2 DM and control groups. Conclusion: The G894T, M235T and T344C polymorphism were not associated with T2 DM. G894T and T344C polymorphisms had an effect on AIx and BP respectively in T2 DM patients.

NITRIC-oxide synthases; ALDOSTERONE; GENETIC polymorphisms; TYPE 2 diabetes; ARTERIAL diseases

Malaysian Journal of Medical Sciences, 2007, Vol 14, p48

1394-195X

Academic Journal