ANTIDIABETIC EFFECT AND HAEMATOLOGICAL CHANGES IN ALLOXAN INDUCED DIABETIC RATS TREATED WITH TRASH FISH, ODONUS NIGER LIVER OIL IN COMPARISION WITH COD LIVER OIL.

Giruba, K. Gowri; Barathkumar, S.; Immanuel, G.; Sivagnanavelmurugan, M.

: Diabetic mellitus (DM) is a metabolic disorder characterized by hyperglycemia, insulin resistance and dyslipidemia. There are several natural products have been identified to treat the DM, fish oils are one of the natural products, which can control DM. In the present study the efficacy of the trash fish Odonus niger liver oil was tested to control DM in Wistar albino rats. Initially, the Wistar albino rats were induced to create type 2 diabetics with alloxan (120mg/kg). Then the rats were grouped into nine sets, with six numbers in each group. Followed by the first three groups of rats were administered orally with three different dosages (500, 750 and 1000mg/kg) of O. niger liver oil, similarly, the other three groups of rats were administered the same doses of cod liver oil. Simultaneously one group of rats was administered with the standard drug glibenclamide (500µg/kg). In the remaining two groups, one set was considered as normal rats (no inducement of diabetic & no treatment) and another set of diabetic control group (diabetic induced, but no treatment). All the groups of experimental and control rats were fed with basal diet during the study period of 8 weeks. Body weight and blood glucose level were recorded once in a week. At the end of the study, i.e. after 56 days of treatment, blood samples were collected from the individual group of rats to determine the hematological and biochemical changes and the rats were sacrificed to collect the kidney samples for enzyme analysis. Pancreatic tissue samples were also collected for histological studied. The study revealed that both O. niger and cod liver oils exhibited an excellent antidiabetic activity, when compared with non-treated diabetic control and standard drug glibenclamide treated animals. The experimental rats administrated with both O. niger and cod liver oils significantly decreased the level of blood glucose, lipid profile (total triglycerides, total cholesterol, LDL &VLDL) and increased the hematological parameters (RBC, WBC, HGB & HCT) and HDL level with respect to increase in concentrations of oils. Administration of both O. niger and cod liver oils protected the liver and kidney functions by reducing the ALP, ALT, LDH, AST, creatinine, uric acid and urea and increasing the protein level in response with increase in concentrations of oils. Further, it was evidenced that both oils protect the β-cells in the pancreas. All the above results suggested that, O. niger liver oil possessed equally good antidiabetic activity as like that of cod liver oil and standard drug glibenclamide.

Biochemical & Cellular Archives, 2023, Vol 23, Issue 1, p575

0972-5075

Academic Journal

10.51470/bca.2023.23.1.575