Title

ISOLATION, GENETIC IDENTIFICATION AND DETECTION OF LIPOLYTIC BACTERIAL STRAINS FROM VARIOUS SOURCES OF MILK PRODUCTS.

Authors

Kareem Hussein Al-Tameemi, Khulood Abdul; AlKabee, Hanaa Jaffer; Madhkoor, Huda Abdulrahim; Ali Wasfi, Rafid Mohammad

Abstract

The increase in industrial demand for lipase has resulted in the discovery of new sources of unusual properties of enzymes. Lipases are the most important enzymes in numerous areas of life, occupy a popular biocatalyst situation and have a wide variety of biotechnological applications. A total of twenty-five samples of milk products, Several kinds of expired imports of dairy products, yogurt, butter, cheese, and cream were tested for screening of microbial lipases producers in the present study. Rhodamine B-olive oil-agar and Tween 80 agar plate were used for the investigation of lipase-producing bacteria. Morphological and Molecular features for the extracellular esterase-producing bacteria were identified. In the present study, two strains “S3 and S4” were identified as Lactococcus lactis strain LLKS3 with Genbank acc. MK977608.1; Lactococcus lactis strain LLKS4 with Genbank acc. MK977609.1 respectively were shown high activity of lipase according to experimental circumstances.

Subjects

LIPASES; LIPOLYTIC enzymes; DAIRY products; RHODAMINE B; LACTOCOCCUS lactis; ENZYMES; EXTRACELLULAR enzymes

Publication

Biochemical & Cellular Archives, 2021, Vol 21, Issue 2, p4143

ISSN

0972-5075

Publication type

Academic Journal