Title

Effect of Moringa Oleifera Leaf Extract on Oxidative Stress and Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance.

Authors

Purbawaning Wulandari, Lisa; Santoso, Budi; Purwanto, Bambang; Miftahussurur, Muhammad; Yanuar Annas, Jimmy; Budiono

Abstract

Background: The use of Moringa oleifera as an antioxidant should be investigated as an alternative treatment of oxidative stress and follicular refinement in PCOS with insulin resistance. Purpose: We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the malondialdehyde levels and theca cell thickness of PCOS female rat with insulin resistance. Method: Three month old Rattus norvegicus stran wistar rat weighing 100-130 grams were divided into 5 groups (n = 8). PCOS model obtained by giving injection of testosterone propionate for 28 days, followed by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/KgBW for 14 days. Then, we analyzed levels of malondialdehyde in the blood and the thickness of theca cell. Results: Malondialdehyde levels in the PCOS control group (5.694±1.464) increased significantly (p <0.05) compared to the normal controls (1.939 ±0.341). Leaf extract Moringa oleifera 500 mg/KgBW (1.982±0.383) showed a significant decrease (p <0.05) to malondialdehyde levels compared to the PCOS control group. Examination of ovarium histology showed that leaf extract Moringa oleifera 500 mg/KgBW (0.931±0.457) significantly decreased the thickness of theca cells (p <0.05) compared to the PCOS control group. Conclusion: Moringa oleifera leaf extract as an antioxidant proven to decrease the malondialdehyde levels and the thickness of theca cell of the female rat model of PCOS.

Subjects

THERAPEUTIC use of antioxidants; POLYCYSTIC ovary syndrome; CONFIDENCE intervals; TESTOSTERONE; OVUM; ANIMAL experimentation; OXIDATIVE stress; RATS; MALONDIALDEHYDE; LEAVES; OVARIAN follicle; DESCRIPTIVE statistics; PLANT extracts; DATA analysis software; ODDS ratio; INSULIN resistance

Publication

Medico-Legal Update, 2021, Vol 21, Issue 2, p744

ISSN

0971-720X

Publication type

Academic Journal

DOI

10.37506/mlu.v21i2.2771