Title

NOVEL POLYDIOXANONE - PLURONIC 127 OXYTOCIN IMPREGNATED MESH, PRELIMINARY STUDY OF OXYTOCIN RELEASE IN VITRO.

Authors

MIRON (LUNGU), ANDREEA ELENA; MOLDOVAN, MARIOARA; PRODAN, DOINA; VLASSA, MIHAELA; MOLDOVAN, MĂDĂLINA; ROMAN, RAREŞ CĂLIN; BADEA, MÎNDRA EUGENIA

Abstract

Polymers represent a novel alternative in bone regeneration. Polydioxanone combined with Pluronic was used as a carrier for Oxytocin in form of an interfacial deposition obtained scaffold. The low rate of resorption of the scaffold follow the bone regeneration sequence assuring a constant and continuous release of Oxytocin. The Oxytocin release was monitored by RP-HPLC method. The separation was performed on a Phenomenex Luna 5µm C18 100A, 250x4.6mm, using isocratic elution with acetonitrile: phosphate buffer pH 7 (30:70 v/v) mobile phase. Flow rate 1 ml / min, column temperature 30°C, DAD, λ = 220nm. The surface morphology of polydioxanone - pluronic 127 - Oxytocin impregnated mesh was characterized by SEM. The preliminary results show that the Polydioxanone combined with Pluronic is a promising polymer for bone regeneration engineering.

Subjects

OXYTOCIN; BONE regeneration; ACETONITRILE; SURFACE morphology; HYDROCHLOROTHIAZIDE; REVERSE phase liquid chromatography; POLYMERS

Publication

Studia Universitatis Babes-Bolyai, Chemia, 2020, Vol 65, Issue 4, p63

ISSN

1224-7154

Publication type

Academic Journal

DOI

10.24193/subbchem.2020.4.05