Title

ID 56. Activity of Flavonoid Compounds from Eriocaulon cinereum R.Br on Breast Cancer Cells (T47D).

Authors

Nugraha, Arde Toga

Abstract

Introduction: Worldwide, breast cancer covers 25.5% of all cancers in women. In 2013, the prevalence of breast cancer was 1.4% or an estimated 347,792 people in the population of all ages in Indonesia. Therefore, effective alternative therapies for cancer are needed with minimal side effects. Plant metabolite compounds have shown characteristic inhibition of cancer cell activity, such as inhibiting the proliferation of cancer cells and inducing apoptosis. Objectives: Aim of this study to explore the cytotoxic activity of flavonoid compounds contained in Eriocaulon cinereum R.Br against breast cancer cells (T47D). Material and Methods: Eriocaulon cinereum was collected from bangka belitung island. It was extracted with ethyl acetate and fractionated with dichloromethane using Vacuum Liquid Chromatography (VLC). Flavonoid compounds were separated from the dichloromethane fraction of Eriocaulon cinerum using the Preparative Thin Layer Chromatography. Identification of compounds was carried out with the thin layer chromatography to ensure the presence of flavonoid. The evaluation of cytotoxic activity was carried out with MTT-assay method. Results: This study revealed that the flavonoid compounds of the dichloromethane fraction Eriocaulon cinereum R.Br were successfully separated using Preparative Thin Layer Chromatography and gave a bright green color that appeared under UV366. The result of cytotoxic activity in vitro with MTT-assay showed that the flavonoid compounds contained in Eriocaulon cinereum R.Br were cytotoxic against T47D breast cancer cells with IC50 values of 136.54 µg/ml. Conclusion: Flavonoid compounds contained in Eriocaulon cinereum R.Br have a cytotoxic activity against T47D cells and has the potential to be further developed as an alternative breast cancer therapy.

Subjects

INDONESIA; CANCER cells; BREAST cancer; THIN layer chromatography; ALTERNATIVE treatment for cancer; FLAVONOIDS; LIQUID chromatography

Publication

Journal of Pharmacy & Bioallied Sciences, 2020, Vol 12, p869

ISSN

0976-4879

Publication type

Academic Journal