Title

Solid phase extraction of ß-sitosterol and a-tocopherol from sunflower oil deodorizer distillate using desilicated zeolite.

Authors

Karaoglu, O.; Alpdogan, G.; Ozdemir, I. S.; Ertas, E.

Abstract

In this study, the efficiency of using zeolite-based adsorbents in a solid phase extraction (SPE) procedure of a-tocopherol and ß-sitosterol isolation from Sunflower Oil Deodorizer Distillate (SuDOD) without pre-treatment was investigated. The results showed that 99.2% a-tocopherol and 97.3% ß-sitosterol were successfully isolated as pure fractions from SuDOD, when desilicated ZSM-5-type zeolite (DSiZSM-5) was used as adsorbent on a SPE. A simple and rapid HPLC method for simultaneous a-tocopherol and ß-sitosterol analysis was developed and validated according to AOAC guidelines. It was found that the inclusion of a DSiZSM-5 SPE step increased the precision of the a-tocopherol and ß-sitosterol analysis. In conclusion, DSiZSM-5 zeolite was proven to be an efficient adsorbent which can be used not only for the recovery of a-tocopherol and ß-sitosterol from SuDOD in industrial scale, but also in a laboratory scale clean-up method prior to the analysis of a-tocopherol and ß-sitosterol.

Subjects

BETA-sitosterol; SUNFLOWER seed oil; SOLID phase extraction; ZEOLITES; VITAMIN E; OIL & fat extraction; STEROLS; SORBENTS

Publication

Grasas y Aceites, 2020, Vol 71, Issue 3, p1

ISSN

0017-3495

Publication type

Academic Journal

DOI

10.3989/gya.0570191