Title

Phylogenetic Profile of Staphylococcus aureus mec A and ica A genes Associated with UTI Patients.

Authors

Abbas, Ahmed A. R.; Hamim, Saad S.

Abstract

The latest years indicated an upsurge in the urinary tract infections (UTIs) occurrence instigated by Staphylococcus aureus at the ward of urology. A total of 50 S. aureus isolate from UTI patients during the period from August to December 2018 at the AL-Hussein Teaching Hospital in Nassyriah City, Southern Iraq were assayed to find the presence of mec A & ica A genes by Polymerase Chain Reaction technique. Selection of six PCR products of mec A and ica A genes named primarily (No1, No2, and No3 for each gene) was done and further exposed to the partial sequencing of DNA to aid the targeted genes for following up their probable relation amongst the global records of Genbank and local isolates. The phylogenetic tree that was created by MEGA 6.0 version software showed closed different molecular relationships among the local S. aureus isolates with similar ones around the world of mec A (No.1-No.3) which were closely related to NCBI-BLAST S. aureus strain MRSA 365325 (MH798858.1) at total genetic changes (0.0005-0.0025%). while, ica A gene in local S. aureus human urine isolate (No.1-No.3) showed close interrelation to the NCBIBLAST S. aureus strain Pn2301 (FJ004990.1) at the total genetic changes of about 0.2-1.0%.

Subjects

IRAQ; STAPHYLOCOCCUS aureus; MICROCOCCACEAE; URINARY tract infections; UROLOGY; POLYMERASE chain reaction; GENES; TEACHING hospitals

Publication

Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 10, p3132

ISSN

0976-0245

Publication type

Academic Journal

DOI

10.5958/0976-5506.2019.03410.7