Title

BRMS1 performs an anti-tumor effect on renal cell carcinoma via inhibition of PI3K/AKT/mTOR signaling pathway.

Authors

Ya-nan Cong; Zhen-guo Wang; Lin Zhao

Abstract

Objective: To explore the anti-tumor of breast cancer metastasis suppressor 1 (BRMS1) on renal cell carcinoma. Methods: BRSM1 stabilized overexpressed and knockdown cell models were constructed to detect the effect of BRMS1 overexpression and knockdown on viability rate of 769-P cells. Activation of PI3K/AKT-mTOR-NFκB signaling pathway, expression of cellular apoptosis-related proteins, DNA damage repair-related proteins and angiogenesisrelated proteins were detected using Western blotting analysis. Results: The viability rate of 769-P cells were significantly decreased in BRMS1 overexpression cells while significantly increased in BRMS1 knockdown cells (P<0.05). The activity of PI3K/AKT-mTOR-NFκB signaling pathway was significantly inhibited with BRMS1 overexpression (P<0.05), and expression of BAX was significantly increased with significantly decreased expression of Bcl-2 (P<0.05). Besides, BRMS1 overexpression could significantly decrease the expression of DNA damage repair-related proteins and angiogenesis and cell-matrix degradation related proteins (P<0.05). Conclusion: BRMS1 induces apoptosis of renal cell carcinoma and performs an anti-tumor effect via inhibition of PI3K/AKT-mTOR-NFκB signaling pathway, expression of DNA damage repair-related proteins and angiogenesis-related proteins.

Subjects

BREAST cancer; METASTASIS; RENAL cell carcinoma; APOPTOSIS; DNA damage

Publication

Journal of Hainan Medical University, 2019, Vol 25, Issue 16, p1

ISSN

1007-1237

Publication type

Academic Journal