Anti-inflammatory effect of withaferin A on dopaminergic neuron of aged rat.

Banu, Mohammad Raziya; Ibrahim, Muhammed; Prabu, K.; Rajasankar, Srinivasagam

Objective: The objective of the present study is to evaluate the effect of Withaferin A (WA) on aging induced inflammation in the dopaminergic system of the rat brain. Materials and Methods: Wistar albino rats were divided into Group I - young (3 months), Group II - aged (24 months), Group III - aged rat supplemented with WA (50 mg/kg body weight [b. w.] once in a day for 30 days), and Group IV - young rat supplemented with WA (50 mg/kg b. w). Results: The estimation of nitrate and nitrite (NOx) levels revealed significantly increased levels in the aged animal when compared to young. The estimation of superoxide (O2-) showed significantly increased in the midbrain and striatum of aged rat when compared to young. The estimation of pro- and antiinflammatory cytokines presented significantly increased levels in aged midbrain and striatum when compared to young. The apoptotic study also revealed increased apoptotic nuclear morphology in substantia nigra and striatum of aged rat when compared to young. Interestingly, the WA administration significantly reversed the NOx, O2- levels. Conclusion: The present data clearly demonstrate that the WA potentially repress the aging mediated oxidative stress and inflammation in the dopaminergic neuron, thereby it prevents aging induced neurodegeneration of dopaminergic neurons.

DOPAMINERGIC neurons; SUBSTANTIA nigra; RATS; BODY weight; OXIDATIVE stress

Drug Invention Today, 2019, Vol 12, Issue 10, p2352

0975-7619

Academic Journal