Effect of chebulinic acid on Bcl-2 and p53 protein expression in A431 human skin cancer cells in vitro.

Rekha, U. Vidhya; Anita, M.; Bhuminathan, S.; Jayamathi, G.; Sadhana, K.; Bhuvaneswarri, J.; Ramya, V.; Paddmanabhan, Preethe; Jayaraman, Selvaraj; Priscilla, D.

Aim: The present study was designed to evaluate the effect of chebulinic acid on Bcl-2 family protein and p53 expression in A431 human skin cancer cells in vitro. Cell Culture and Treatment: The skin cancer cell line, A431 was purchased from the National Centre for Cell Sciences, Pune, India, and cultured in Dulbecco’s Modified Eagle’s Medium supplemented with fetal bovine serum, penicillin G, and streptomycin. The cells were placed in 5% CO2 incubator and the further experiments were started after the confluency stage was attained. The cells were incubated at 37°C with the various doses of chebulinic acid (10, 25, 50, and 100 μg/ml) in a CO2 incubator to find out the inhibitory concentration of IC50. Results: A431 skin cancer cell line showed a significant increase in the levels of Bcl-2, p-Bcl2, and Bcl-xL whereas decreased in the levels of p53 at the doses of 50 and 100 μg. However, an effective dose of chebulinic acid (100 μg/ml) altered the levels bring back to the normalcy. Conclusion: The anticancer potential of the chebulinic acid is mediated through the controlling of apoptotic signaling molecules such as Bcl-2, Bcl-xL, and tumor suppressor proteins p53. Hence, this study concludes that chebulinic acid can be used as one of the potential therapeutic herbal drugs for the treatment of skin cancer. Further studies on the molecular mechanisms of the action of chebulinic acid are warranted to ascertain its potential.

PUNE (India); BCL-2 proteins; SKIN cancer; TUMOR suppressor proteins; CANCER cells; PROTEIN expression; P53 protein

Drug Invention Today, 2019, Vol 11, Issue 10, p2640

0975-7619

Academic Journal