Lupeol exerts its antidiabetic activity through insulin receptor and glucose transporter-4 in gracilis muscle in high fat and sucrose-induced diabetic rats.

Kottireddy, Satyanarayana; Koora, Sravanthi; J., Selvaraj

Background: Lupeol is one of the natural herbal drugs, which is abundant in edible vegetables, fruits, and also in medicinal plants. It has been shown to reduce hyperglycemia and hyperinsulinemia in experimental mice model. However, the molecular mechanism by which it exhibits antidiabetic property is not known. Aim: The study was aimed at assessing the antidiabetic activity of lupeol on insulin receptor (IR) and glucose transporter-4 (GLUT-4) expression in gracilis muscle of high-fat diet and sucrose-induced type-2 diabetic animals. Materials and Methods: The oral effective dose of lupeol (25 mg/kg body weight) was given once a day until the end of the study (60 days post-induction of diabetes) to high-fat diet-induced diabetic rats. At the end of the experimental period, fasting blood glucose, oral glucose tolerance, serum lipid profile, oxidative stress markers, and the levels of insulin signaling molecules (IR and GLUT-4) and tissue glycogen in the gracilis muscle were assessed. Results: Diabetic rats showed impaired glucose tolerance and impairment in insulin signaling molecules (IR and GLUT-4) and glycogen concentration. Serum insulin, lipid profile, and oxidative stress markers were found to be increased in diabetic control rats. The treatment with lupeol restored effectively the altered levels of insulin signaling molecules and serum insulin along with the biochemical profiles. Conclusion: Our present finding indicates that the antidiabetic activity of lupeol is mediated through the attenuation of type-2 diabetes-induced changes in oxidative stress, lipid profile, and glycogen concentration as well as altered levels of IR and GLUT-4 expression in the gracilis muscle. Lupeol has the beneficial role for the management of type-2 diabetes. Further studies on the effect of lupeol on insulin downstream signaling molecules need to be studied to ascertain its potential antidiabetic activity.

STREPTOZOTOCIN; INSULIN receptors; GLUCOSE; GLYCEMIC control; BLOOD sugar; BLOOD lipids

Drug Invention Today, 2019, Vol 11, Issue 9, p2258

0975-7619

Academic Journal