Antiallergic activity of alpha-lipoic acid.

Subramanian, Vaishnavi Sivakali; Priya, V. Vishnu; Gayathri, R.

Aim: The goal of the present study is to determine the alpha-lipoic acid (ALA) prevents the release of beta-hexosaminidase enzyme and proves its antiallergic activity. Materials and Methods: RBL-2H3 cells were cultured in minimum essential medium supplemented. The cells were seeded in 24-wells plate (5 × 105 cells/mL) and incubated to adhere at 37°C in 5% CO2 for 1.5 h. RBL-2H3 cells were sensitized with anti-DNP immunoglobulin E and incubated at 37°C in 5% CO2 for 24 h. The ALA with different concentrations (20, 40, 80m and 100 μg/ml) was dissolved in media and used for the further test. The cells were washed with 400 μL of Siraganian buffer (buffer A). The test sample (20 μL) solution was added to each well and incubated for 10 min, followed by addition of 20 μL of antigen at 37°C for 20 min. The supernatants were transferred into 96-well plate in 50 μL/wells and incubated with 50 μL of substrate p-nitrophenyl- N-acetyl-b-D-glucosaminide (PNAG) at 37°C for 3 h. The reaction was stopped by adding 200 μL of stop solution (0.1 M Na2CO3/NaHCO3, pH 10.0). The absorbance was measured with a microplate reader at 405 nm. Results: Zone of inhibition for different concentrations of ALA increases with concentration with the highest zone of inhibition was seen with the positive control (Chlorpheniramine). Therefore, inhibition of beta-hexosaminidase enzyme is seen with ALA. Conclusion: Hence, ALA inhibits beta-hexosaminidase enzyme which is responsible for allergic and anaphylactic reactions. Although the inhibition is not up to the mark as in chlorpheniramine, further researches with higher concentrations of ALA can be done to evaluate the efficiency of antiallergic activity as equal as chlorpheniramine.

LIPOIC acid; ANAPHYLAXIS; ALLERGIES; CELL culture; MASS media use; IMMUNOGLOBULIN E

Drug Invention Today, 2019, Vol 11, Issue 6, p1499

0975-7619

Academic Journal