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Title: Determination of Gallic Acid in Rotala rotundifolia and Shuijia Zhixue Capsule by HPLC.
Authors: Lali ADU; Xingguo ZHANG; Luncai LUO; Jiaxing CHEN; Feng OUYANG
Abstract: [Objectives] To establish a method for the determination of gallic acid in Shuijia Zhixue capsule and Rotala rotundifolia. [Methods] High performance liquid chromatography (HPLC) and Phenomenex C18 column were used, the mobile phase was 0.1% phosphoric acid: methanol = 85:15, the flow rate was 0.5 mL/min, and the detection wavelength was 270 nm. [Results] In the range of (2-25) µg/mL, the concentration of gallic acid had a good linear relationship with the peak area, r = 0. 990 8; the average recovery was 96.88%. [Conclusions] The method was simple, sensitive and reproducible. It provided a basis for the analysis of gallic acid in Rotala rotundifolia and Shuijia Zhixue capsule and the establishment of quality detection methods in the future.
Subjects: GALLIC acid; HIGH performance liquid chromatography; PHENOLIC acids
Publication: Medicinal Plant, 2019, Vol 10, Issue 4, p60
ISSN: 2152-3924
Publication type: Academic Journal
DOI: 10.19600/j.cnki.issn2152-3924.2019.04.013

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Determination of Gallic Acid in Rotala rotundifolia and Shuijia Zhixue Capsule by HPLC.