A Study on Resveratrol on the Antioxidative and Whitening Cosmeceutical Ingredients.

Mi-Yun Yoon; Ji-Sun Moon; Seon-Hee You

Objectives: Resveratrol is a variety of physiological active substances and is an excellent ingredient in antioxidant, anti-inflammatory and anti-aging action. Therefore, I would like to find out the possibility of adding cosmetics. Method: The cytotoxicity test using MTT solution was carried out to check the safety of reverse control. Also DPPH assay was performed to measure antioxidant activity in resveratrol itself, and ROS was measured to measure antioxidant activity in cells. NO production was measured to observe anti-inflammatory action, and tyrosinase activity measurement and melanin production were measured to measure skin whitening activity. Findings: we were able to confirm the safety of resveratrol's cytotoxicity. At concentration of 1, 10, 100 µg/mL of Resveratrol, the DPPH radical scavenging activity was observed to show high concentration -dependent activity of free radical scavenging. And In order to check the antioxidant properties within the cell, the ROS (reactive oxygen specifications) using DCF-DA were observed to reduce the effects. The addition of resveratrol 1, 10, 100 µg/mL on RAW 264.7 macrophages stimulated with lipopolysaccharide resulted in NO produce inhibition in concentration dependent and a strong inhibition rate of 53% at 100 µg/ mL concentration. Tyrosinase active action was found to inhibited dose dependant. Melanin produce was also prevented by dose dependant. Applications: These results suggest that the active oxygen - induced skin inflammation process is delayed as much as possible, so that it can be utilized as a functional cosmetic material having an effect on skin diseases and skin aging.

ANTI-inflammatory agents; ANTIOXIDANTS; CELL surface antigens; COSMETICS; IMMUNODIAGNOSIS; MELANINS; NITRIC oxide; SKIN aging; RESVERATROL; LIPOPOLYSACCHARIDES; FREE radical scavengers; DESCRIPTIVE statistics

Medico-Legal Update, 2019, Vol 19, Issue 2, p609

0971-720X

Academic Journal

10.5958/0974-1283.2019.00243.3