Effects of the endocrine disrupting chemicals on the trace element and mineral levels in the brain.

Aydemir, Duygu; Karabulut, Gözde; Şimşek, Gülsu; Gök, Müslüm; Barlas, Nurhayat; Ulusu, Nuriye Nuray

Objective: Humans are exposed to the various dangerous chemicals via industrial products including cosmetics, food wrappings, personal care products, medical tubes, plastic bottles, toys etc daily basis. Di(2-Ethylhexyl) (DEHP) is the most commonly used plasticizer in the industry and belongs to the endocrine disrupting chemicals family. Daily human exposure to the DEHP is 3-30 pg/kg and occurs via inhalation, diet and/or dermal absorption. There is an increasing concern against DEHP and its metabolites since their adverse health effects on human and wildlife. Methods: 24 prepubertal male Wistar albino rats were randomly divided into four groups based on the DEHP administration as 0, 100, 200, 400 mg/kg/day. DEHP administration was performed by oral gavage daily. Glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR) and glutathione S-transferase (GST) enzyme activities were measured by spectrophotometer. Trace element (Ag, Al, As, Ba, Be, Cd, Co, Cr, Cs, Cu, Fe, Ga, Li, Mn, Ni, Pb, Rb, Se, Sr, Ti, U, V, Zn) and mineral levels (Ca, K, Mg, Na) were evaluated in the brain samples of DEHP-administered rats via ICP-MS. Results: Sodium (Na), magnesium (Mg), potassium (K), manganese (Mn), rubidium (Rb) and iron (Fe) levels significantly increased in 400 mg/kg/day DEHP treated groups compared to the control in rat brain tissue samples (p<0.0001). Zinc (Zn) and strontium (Sr) levels significantly elevated 1.5 times more in 200 mg/kg/day DEHP treated groups compared to the control (p<0.0001). On the other hand, Cu levels significantly decreased in 200 mg/kg/day group compared to the 100 and 400 mg/kg/day treated groups in brain samples (p<0.0001). This increase was 1.5 times less in 200 mg/kg/day group compared to others. Conclusion: Our data have revealed that, DEHP can disrupt trace element and mineral concentrations, therefore long-term exposure to the DEHP may cause biochemical or physiological impairment in the brain.

TRACE elements; ENDOCRINE disruptors; CHEMICAL elements; ENDOCRINE glands; HYGIENE products; GLUCOSE-6-phosphate dehydrogenase

Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS38

1307-8798

Academic Journal